open access

Vol 89, No 10 (2018)
Research paper
Published online: 2018-10-31
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Common Methylenetetrahydrofolate Reductase Polymorphisms (A1298C & C677T) in Ectopic Trophoblasts and Methotrexate Treatment Failure in Tubal Pregnancies

Emre Zafer1, Nesibe Kahraman Cetin2, Ozgur Deniz Turan1, Evrim Kardelen1, Imran Kurt Omurlu3, Hasan Yuksel1
DOI: 10.5603/GP.a2018.0091
·
Pubmed: 30393840
·
Ginekol Pol 2018;89(10):536-540.
Affiliations
  1. Department of Obstetrics and Gynecology, Adnan Menderes University Hospital, Aytepe, 09100 Aydin, Turkey
  2. Department of Pathology, Adnan Menderes University Hospital, Aytepe, 09100 Aydin, Turkey
  3. Department of Biostatistics, Adnan Menderes University Faculty of Medicine, Aytepe, 09100 Aydin, Turkey

open access

Vol 89, No 10 (2018)
ORIGINAL PAPERS Gynecology
Published online: 2018-10-31

Abstract

Objectives: The success rate of methotrexate (MTX) therapy varies among tubal ectopic pregnancies. Common
methylenetetrahydrofolate reductase (MTHFR) polymorphisms (C677T&A1298C) have been suggested to alter MTX effect. This study aimed to assess and compare MTX treatment failure rates with respect to MTHFR polymorphisms in trophoblasts of ectopic tubal pregnancies.
Material and methods: A retrospective chart review of tubal ectopic pregnancies was conducted and 34 eligible cases were found. Paraffinized blocks of ectopic trophoblastic tissues were retrieved from the archives of pathology department.
Common MTHFR polymorphisms were studied on microdissected trophoblastic tissues. Sixteen cases with history of failed MTX therapy (study group) and 18 control cases were compared for their pertinent clinical characteristics and common MTHFR polymorphisms (C677T&A1298) data.
Results: In the study group, there were 8 (50%) C677T single nucleotide polymorphisms (SNP) and 9 (56.7%) A1298C SNP. Polymorphism rates were not found to be different between two groups for neither polymorphism (p > 0.05 for both). Number of compound heterozygotes was 3 (18.7%) in study group and 5 (27.7%) in controls (p = 0.693). In addition, MTHFR polymorphism presence seemed to have no effect on interval serum β-hCG concentration change in MTX-fail group (p=0.693).
Conclusions: Our data implied that common MTHFR polymorphisms of ectopic trophoblastic tissue are not associated with MTX failure in patients with tubal pregnancies. Additionally, serum β-hCG concentration changes caused by MTX treatment and studied MTHFR polymorphisms are likely independent.

Abstract

Objectives: The success rate of methotrexate (MTX) therapy varies among tubal ectopic pregnancies. Common
methylenetetrahydrofolate reductase (MTHFR) polymorphisms (C677T&A1298C) have been suggested to alter MTX effect. This study aimed to assess and compare MTX treatment failure rates with respect to MTHFR polymorphisms in trophoblasts of ectopic tubal pregnancies.
Material and methods: A retrospective chart review of tubal ectopic pregnancies was conducted and 34 eligible cases were found. Paraffinized blocks of ectopic trophoblastic tissues were retrieved from the archives of pathology department.
Common MTHFR polymorphisms were studied on microdissected trophoblastic tissues. Sixteen cases with history of failed MTX therapy (study group) and 18 control cases were compared for their pertinent clinical characteristics and common MTHFR polymorphisms (C677T&A1298) data.
Results: In the study group, there were 8 (50%) C677T single nucleotide polymorphisms (SNP) and 9 (56.7%) A1298C SNP. Polymorphism rates were not found to be different between two groups for neither polymorphism (p > 0.05 for both). Number of compound heterozygotes was 3 (18.7%) in study group and 5 (27.7%) in controls (p = 0.693). In addition, MTHFR polymorphism presence seemed to have no effect on interval serum β-hCG concentration change in MTX-fail group (p=0.693).
Conclusions: Our data implied that common MTHFR polymorphisms of ectopic trophoblastic tissue are not associated with MTX failure in patients with tubal pregnancies. Additionally, serum β-hCG concentration changes caused by MTX treatment and studied MTHFR polymorphisms are likely independent.

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Keywords

tubal pregnancy; methotrexate; methylenetetrahydrofolate reductase; polymorphism; trophoblast

About this article
Title

Common Methylenetetrahydrofolate Reductase Polymorphisms (A1298C & C677T) in Ectopic Trophoblasts and Methotrexate Treatment Failure in Tubal Pregnancies

Journal

Ginekologia Polska

Issue

Vol 89, No 10 (2018)

Article type

Research paper

Pages

536-540

Published online

2018-10-31

DOI

10.5603/GP.a2018.0091

Pubmed

30393840

Bibliographic record

Ginekol Pol 2018;89(10):536-540.

Keywords

tubal pregnancy
methotrexate
methylenetetrahydrofolate reductase
polymorphism
trophoblast

Authors

Emre Zafer
Nesibe Kahraman Cetin
Ozgur Deniz Turan
Evrim Kardelen
Imran Kurt Omurlu
Hasan Yuksel

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