open access

Vol 89, No 3 (2018)
Research paper
Published online: 2018-03-30
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PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women

Magdalena Frej-Mądrzak1, Anna Gryboś2, Marian Gryboś2, Dorota Teryks-Wołyniec1, Agnieszka Jama-Kmiecik1, Jolanta Sarowska1, Irena Choroszy-Król1
DOI: 10.5603/GP.a2018.0020
·
Pubmed: 29664545
·
Ginekol Pol 2018;89(3):115-119.
Affiliations
  1. Department of Basic Sciences Faculty of Health Sciences Wroclaw Medical University, ul. Chałubińskiego 4,, 50-368 Wrocław, Poland
  2. 1st Department and Clinic of Gynaecology and Obstetrics Faculty of Medicine Wroclaw Medical University, ul. T. Chałubińskiego 3, 50-368 Wrocław, Poland

open access

Vol 89, No 3 (2018)
ORIGINAL PAPERS Gynecology
Published online: 2018-03-30

Abstract

Objectives:  Chlamydial  infection  is  often  asymptomatic.  The  lack  of  symptoms  may  result  in  the  infection  developing  into  a  chronic.  Chlamydial  infections  of  the  genitourinary  system  in  women  can  lead  to  serious  complications  like  PID,  fallopian  tubes  infertility,  ectopic  pregnancy,  and  chronic  pelvic  pain.  An  infection  of  the  genitourinary  system  does  not  cause  any  lasting  immune  resistance  and  does  not  protect  against  re-infection.  The  aim  of  this  research  was  to  conduct  tests  for  Chlamydia  trachomatis  on  healthy  women  without  any  genital  system  symptoms  and  to  estimate  the  frequency  of  asymptomatic  infections.  Material  and  methods:  During  preventive  examinations  a  cervical  smear  was  obtained  from  the  patients  n  =  100.  The  aver­age  age  of  the  patients  was  24.86  ±  3.15.  The  swabs  were  sampled  by  gynecologists.  During  the  examinations  Geneproof  PathogenFree  DNA  isolation  Kit  and  GeneProof  Chlamydia  trachomatis  PCR  kit  which  detects  16S  rRNA  conservative  coding  sequence,  conservative  region  of  cryptic  plasmid  DNA,  including  deletion  mutation  in  cryptic  plasmid  (Swedish  variant).  Results:  Swabs  were  sampled  from  100  women  aged  18–32  who  had  no  symptoms  of  chlamydial  genitourinary  system  infection.  Within  the  study  group  4%  of  women  received  a  positive  result,  i.e.  4/100.  Conclusions:  The  study  confirmed  asymptotic  infection  in  4%  of  women.  In  own  research  it  was  not  possible  to  confirm  cor­relation  between  the  presence  of  Chlamydia  trachomatis  and  the  number  of  partners  or  the  number  of  sexual  intercourses.

Abstract

Objectives:  Chlamydial  infection  is  often  asymptomatic.  The  lack  of  symptoms  may  result  in  the  infection  developing  into  a  chronic.  Chlamydial  infections  of  the  genitourinary  system  in  women  can  lead  to  serious  complications  like  PID,  fallopian  tubes  infertility,  ectopic  pregnancy,  and  chronic  pelvic  pain.  An  infection  of  the  genitourinary  system  does  not  cause  any  lasting  immune  resistance  and  does  not  protect  against  re-infection.  The  aim  of  this  research  was  to  conduct  tests  for  Chlamydia  trachomatis  on  healthy  women  without  any  genital  system  symptoms  and  to  estimate  the  frequency  of  asymptomatic  infections.  Material  and  methods:  During  preventive  examinations  a  cervical  smear  was  obtained  from  the  patients  n  =  100.  The  aver­age  age  of  the  patients  was  24.86  ±  3.15.  The  swabs  were  sampled  by  gynecologists.  During  the  examinations  Geneproof  PathogenFree  DNA  isolation  Kit  and  GeneProof  Chlamydia  trachomatis  PCR  kit  which  detects  16S  rRNA  conservative  coding  sequence,  conservative  region  of  cryptic  plasmid  DNA,  including  deletion  mutation  in  cryptic  plasmid  (Swedish  variant).  Results:  Swabs  were  sampled  from  100  women  aged  18–32  who  had  no  symptoms  of  chlamydial  genitourinary  system  infection.  Within  the  study  group  4%  of  women  received  a  positive  result,  i.e.  4/100.  Conclusions:  The  study  confirmed  asymptotic  infection  in  4%  of  women.  In  own  research  it  was  not  possible  to  confirm  cor­relation  between  the  presence  of  Chlamydia  trachomatis  and  the  number  of  partners  or  the  number  of  sexual  intercourses.

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Keywords

Chlamydia trachomatis, asymptomatic infection in woman, PCR diagnostic

About this article
Title

PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women

Journal

Ginekologia Polska

Issue

Vol 89, No 3 (2018)

Article type

Research paper

Pages

115-119

Published online

2018-03-30

DOI

10.5603/GP.a2018.0020

Pubmed

29664545

Bibliographic record

Ginekol Pol 2018;89(3):115-119.

Keywords

Chlamydia trachomatis
asymptomatic infection in woman
PCR diagnostic

Authors

Magdalena Frej-Mądrzak
Anna Gryboś
Marian Gryboś
Dorota Teryks-Wołyniec
Agnieszka Jama-Kmiecik
Jolanta Sarowska
Irena Choroszy-Król

References (20)
  1. Menon S, Timms P, Allan JA, et al. Human and Pathogen Factors Associated with Chlamydia trachomatis-Related Infertility in Women. Clin Microbiol Rev. 2015; 28(4): 969–985.
  2. Gouvras G. The European Centre for Disease Prevention and Control. Eurosurveillance. 2004; 9(10): 1–2.
  3. 2006 UK National Guideline for the Management of Genital Tract Infection with Chlamydia trachomatis. http://www.nordhavenclinic.co.uk/chlamydia.PDF (2006).
  4. Lanjouw E, Ouburg S, de Vries HJ, et al. 2015 European guideline on the management of Chlamydia trachomatis infections. Int J STD AIDS. 2016; 27(5): 333–348.
  5. Sexually Transmitted Diseases Treatment Guidelines, 2010; 59; RR-12. https://www.cdc.gov/mmwr/pdf/rr/rr5912.pdf (2010).
  6. Tiplica GS, Radcliffe K, Evans C, et al. 2015 European guidelines for the management of partners of persons with sexually transmitted infections. J Eur Acad Dermatol Venereol. 2015; 29(7): 1251–1257.
  7. Evans R. European Centre for Disease Prevention and Control. Nursing Standard. 2014; 29(9): 30–30.
  8. Nwokolo NC, Dragovic B, Patel S, et al. 2015 UK national guideline for the management of infection with Chlamydia trachomatis. Int J STD AIDS. 2016; 27(4): 251–267.
  9. Chojnacka K, Szczapa J, Kędzia W. Częstość perinatalnej transmisji Chlamydia trachomatis i powikłania z nią związane u noworodków przedwcześnie urodzonych. Ginekol Pol. 2012; 83: 116–121.
  10. Brown MA, Potroz MG, Teh SW, et al. Natural Products for the Treatment of Chlamydiaceae Infections. Microorganisms. 2016; 4(4).
  11. Rekomendacje Polskiego Towarzystwa Ginekologicznego dotyczące zakażeń Chlamydia trachomatis w położnictwie i ginekologii. Ginekol Pol. 2007; 78: 574–575.
  12. Bianchi S, Frati ER, Canuti M, et al. Molecular epidemiology and genotyping ofinfection in a cohort of young asymptomatic sexually active women (18-25 years) in Milan, Italy. J Prev Med Hyg. 2016; 57(3): E128–E134.
  13. Dudareva-Vizule S, Haar K, Sailer A, et al. Chlamydia trachomatis laboratory sentinel team. Establishment of a voluntary electronic Chlamydia trachomatis laboratory surveillance system in Germany, 2008 to 2014. Euro Surveill. 2017; 22(6).
  14. Arsić D, Milovanović DR, Ferati AB, et al. Monitoring of Chlamydia Trachomatis Genitourinary Infection in Women - Analytical Comparative Study Using Public Health Records from Two Balkan Countries. Cent Eur J Public Health. 2016; 24(1): 16–21.
  15. Frej-Mądrzak M, Teryks-Wołyniec D, Jama-Kmiecik A, et al. Zakażenia układu moczowo-płciowego oraz spojówek Chlamydia trachomatis u dorosłych i dzieci w latach 2012–2013. Fam Med Prim Care Rev. 2014; 16(3): 222–224.
  16. Berntsson M, Tunbäck P. Clinical and microscopic signs of cervicitis and urethritis: correlation with Chlamydia trachomatis infection in female STI patients. Acta Derm Venereol. 2013; 93(2): 230–233.
  17. Wojtyniak B, Goryński P, Moskalewicz B. Sytuacja zdrowotna ludności Polski i jej uwarunkowania. Nowotwory. Journal of Oncology. 2013; 63(3): 269–269.
  18. Młynarczyk- Bonikowska B, Skulska E, Walter de Walthoffen S, et al. Współistnienie zakażeń Neisseria gonorhoeae i Chlamydia trachomatis u pacjentów zgłaszających się do Kliniki Dermatologii i wenerologii Warszawskiego Uniwersytetu Medycznego. Med Dośw Mikrobiol. 2015; 67: 89–95.
  19. Torrone E, Johnson R, Tian L, et al. Prevalence of Neisseria gonorrhoeae Among Persons 14 to 39 Years of Age, United States, 1999 to 2008. Sexually Transmitted Diseases. 2013; 40(3): 202–205.
  20. Machado MS, Costa e Silva BF, Gomes IL, et al. Prevalence of cervical Chlamydia trachomatis infection in sexually active adolescents from Salvador, Brazil. Braz J Infect Dis. 2012; 16(2): 188–191.

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