Vol 89, No 3 (2018)
Research paper
Published online: 2018-03-30

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PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women

Magdalena Frej-Mądrzak1, Anna Gryboś2, Marian Gryboś2, Dorota Teryks-Wołyniec1, Agnieszka Jama-Kmiecik1, Jolanta Sarowska1, Irena Choroszy-Król1
Pubmed: 29664545
Ginekol Pol 2018;89(3):115-119.

Abstract

Objectives:  Chlamydial  infection  is  often  asymptomatic.  The  lack  of  symptoms  may  result  in  the  infection  developing  into  a  chronic.  Chlamydial  infections  of  the  genitourinary  system  in  women  can  lead  to  serious  complications  like  PID,  fallopian  tubes  infertility,  ectopic  pregnancy,  and  chronic  pelvic  pain.  An  infection  of  the  genitourinary  system  does  not  cause  any  lasting  immune  resistance  and  does  not  protect  against  re-infection.  The  aim  of  this  research  was  to  conduct  tests  for  Chlamydia  trachomatis  on  healthy  women  without  any  genital  system  symptoms  and  to  estimate  the  frequency  of  asymptomatic  infections.  Material  and  methods:  During  preventive  examinations  a  cervical  smear  was  obtained  from  the  patients  n  =  100.  The  aver­age  age  of  the  patients  was  24.86  ±  3.15.  The  swabs  were  sampled  by  gynecologists.  During  the  examinations  Geneproof  PathogenFree  DNA  isolation  Kit  and  GeneProof  Chlamydia  trachomatis  PCR  kit  which  detects  16S  rRNA  conservative  coding  sequence,  conservative  region  of  cryptic  plasmid  DNA,  including  deletion  mutation  in  cryptic  plasmid  (Swedish  variant).  Results:  Swabs  were  sampled  from  100  women  aged  18–32  who  had  no  symptoms  of  chlamydial  genitourinary  system  infection.  Within  the  study  group  4%  of  women  received  a  positive  result,  i.e.  4/100.  Conclusions:  The  study  confirmed  asymptotic  infection  in  4%  of  women.  In  own  research  it  was  not  possible  to  confirm  cor­relation  between  the  presence  of  Chlamydia  trachomatis  and  the  number  of  partners  or  the  number  of  sexual  intercourses.

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