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PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women
Affiliations- Department of Basic Sciences Faculty of Health Sciences Wroclaw Medical University, ul. Chałubińskiego 4,, 50-368 Wrocław, Poland
- 1st Department and Clinic of Gynaecology and Obstetrics Faculty of Medicine Wroclaw Medical University, ul. T. Chałubińskiego 3, 50-368 Wrocław, Poland
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Abstract
Objectives: Chlamydial infection is often asymptomatic. The lack of symptoms may result in the infection developing into a chronic. Chlamydial infections of the genitourinary system in women can lead to serious complications like PID, fallopian tubes infertility, ectopic pregnancy, and chronic pelvic pain. An infection of the genitourinary system does not cause any lasting immune resistance and does not protect against re-infection. The aim of this research was to conduct tests for Chlamydia trachomatis on healthy women without any genital system symptoms and to estimate the frequency of asymptomatic infections. Material and methods: During preventive examinations a cervical smear was obtained from the patients n = 100. The average age of the patients was 24.86 ± 3.15. The swabs were sampled by gynecologists. During the examinations Geneproof PathogenFree DNA isolation Kit and GeneProof Chlamydia trachomatis PCR kit which detects 16S rRNA conservative coding sequence, conservative region of cryptic plasmid DNA, including deletion mutation in cryptic plasmid (Swedish variant). Results: Swabs were sampled from 100 women aged 18–32 who had no symptoms of chlamydial genitourinary system infection. Within the study group 4% of women received a positive result, i.e. 4/100. Conclusions: The study confirmed asymptotic infection in 4% of women. In own research it was not possible to confirm correlation between the presence of Chlamydia trachomatis and the number of partners or the number of sexual intercourses.
Abstract
Objectives: Chlamydial infection is often asymptomatic. The lack of symptoms may result in the infection developing into a chronic. Chlamydial infections of the genitourinary system in women can lead to serious complications like PID, fallopian tubes infertility, ectopic pregnancy, and chronic pelvic pain. An infection of the genitourinary system does not cause any lasting immune resistance and does not protect against re-infection. The aim of this research was to conduct tests for Chlamydia trachomatis on healthy women without any genital system symptoms and to estimate the frequency of asymptomatic infections. Material and methods: During preventive examinations a cervical smear was obtained from the patients n = 100. The average age of the patients was 24.86 ± 3.15. The swabs were sampled by gynecologists. During the examinations Geneproof PathogenFree DNA isolation Kit and GeneProof Chlamydia trachomatis PCR kit which detects 16S rRNA conservative coding sequence, conservative region of cryptic plasmid DNA, including deletion mutation in cryptic plasmid (Swedish variant). Results: Swabs were sampled from 100 women aged 18–32 who had no symptoms of chlamydial genitourinary system infection. Within the study group 4% of women received a positive result, i.e. 4/100. Conclusions: The study confirmed asymptotic infection in 4% of women. In own research it was not possible to confirm correlation between the presence of Chlamydia trachomatis and the number of partners or the number of sexual intercourses.
Keywords
Chlamydia trachomatis, asymptomatic infection in woman, PCR diagnostic
About this articleTitle
PCR diagnostics of Chlamydia trachomatis in asymptomatic infection by women
Journal
Issue
Article type
Research paper
Pages
115-119
Published online
2018-03-30
Page views
2389
Article views/downloads
1794
DOI
10.5603/GP.a2018.0020
Pubmed
Bibliographic record
Ginekol Pol 2018;89(3):115-119.
Keywords
Chlamydia trachomatis
asymptomatic infection in woman
PCR diagnostic
Authors
Magdalena Frej-Mądrzak
Anna Gryboś
Marian Gryboś
Dorota Teryks-Wołyniec
Agnieszka Jama-Kmiecik
Jolanta Sarowska
Irena Choroszy-Król
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