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Comparison of whole genome expression profile between preterm and full-term newborns
Affiliations- Department of Pediatrics, Chair of Pediatrics, Jagiellonian Univeristy Medical College, Krakow, Poland
- Klinika Neonatologii i Intensywnej Terapii Noworodka, Warszawski Uniwersytet Medyczny, Karowa 2, 00315 Warszawa, Poland
- Katedra Pediatrii, Zakład Genetyki Medycznej, Uniwersytet Jagielloński, Collegium Medicum, Wielicka 265, 30663 Kraków, Poland
- Oslo Computing Center, Gaustadalleen 23a, 0373 Oslo, Norway
- Department of Pediatric Research, Oslo University Hospital and University of Oslo, Sognsvannsveien 20, 0372 Oslo, Norway
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Abstract
Objectives: Evaluate the time dependent expression of genes in preterm neonates and verify the influence of ontogenic maturation and the environmental factors on the gene expression after birth. Material and methods: The study was carried out on 20 full-term newborns and 62 preterm newborns (mean birth weight = 1002 [g] (SD: 247), mean gestational age = 27.2 weeks (SD: 1.9)). Blood samples were drawn from all the study participants at birth and at the 36th week postmenstrual age from the preterm group to assess whole genome expression in umbilical cord blood and in peripheral blood leukocytes, respectively. (SurePrint G3 Human Gene Expression v3, 8x60K Microarrays (Agilent)).
Results: A substantial number of genes was found to be expressed differentially at the time of birth and at 36 PMA in comparison to the term babies with more genes being down-regulated than up-regulated. However, the fold change in the majority of cases was < 2.0. Extremely preterm and very preterm infants were characterized by significantly down-regulated cytokine and chemokine related pathways. The number of down-regulated genes decreased and number of up-regulated genes increased at 36 PMA vs. cord blood. There were no specific gene expression pathway profiles found within the groups of different gestational ages.
Conclusions: Preterm delivery is associated with a different gene expression profile in comparison to term delivery. The gene expression profile changes with the maturity of a newborn measured by the gestational age.
Abstract
Objectives: Evaluate the time dependent expression of genes in preterm neonates and verify the influence of ontogenic maturation and the environmental factors on the gene expression after birth. Material and methods: The study was carried out on 20 full-term newborns and 62 preterm newborns (mean birth weight = 1002 [g] (SD: 247), mean gestational age = 27.2 weeks (SD: 1.9)). Blood samples were drawn from all the study participants at birth and at the 36th week postmenstrual age from the preterm group to assess whole genome expression in umbilical cord blood and in peripheral blood leukocytes, respectively. (SurePrint G3 Human Gene Expression v3, 8x60K Microarrays (Agilent)).
Results: A substantial number of genes was found to be expressed differentially at the time of birth and at 36 PMA in comparison to the term babies with more genes being down-regulated than up-regulated. However, the fold change in the majority of cases was < 2.0. Extremely preterm and very preterm infants were characterized by significantly down-regulated cytokine and chemokine related pathways. The number of down-regulated genes decreased and number of up-regulated genes increased at 36 PMA vs. cord blood. There were no specific gene expression pathway profiles found within the groups of different gestational ages.
Conclusions: Preterm delivery is associated with a different gene expression profile in comparison to term delivery. The gene expression profile changes with the maturity of a newborn measured by the gestational age.
Keywords
transcriptome, very low birth weight, cord blood
About this articleTitle
Comparison of whole genome expression profile between preterm and full-term newborns
Journal
Issue
Article type
Research paper
Pages
434-441
Published online
2017-08-31
Page views
1946
Article views/downloads
1460
DOI
Pubmed
Bibliographic record
Ginekol Pol 2017;88(8):434-441.
Keywords
transcriptome
very low birth weight
cord blood
Authors
Przemko Kwinta
Renata Bokiniec
Mirosław Bik-Multanowski
Clara-Cecilie Gunther
Agnieszka Grabowska
Teofila Książek
Anna Madetko-Talowska
Katarzyna Szewczyk
Monika Szwarc-Duma
Maria K. Borszewska-Kornacka
Lars O. Baumbusch
Cecilie Revhaug
Ola D. Saugstad
Jacek J. Pietrzyk
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