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Cytohistological discrepancies of cervico-vaginal smears and HPV status
Affiliations- Gulhane Military Medical Faculty, Department of Obstetrics and Gynecology, Etlik-Ankara, Turkey, Türkiye
- Etimesgut Military Hospital, Department of Obstetrics and Gynecology, Etlik-Ankara, Turkey
- Hakkari Military Hospital, Department of Obstetrics and Gynecology, Hakkari, Turkey
open access
Abstract
Objectives: Discrepancies between abnormal cervical cytology or high-risk human papillomavirus (HR-HPV) status (cytolo-gy negative/HPV positive) and subsequent histological findings are a common occurrence. After using co-testing, the discrepancies between the HR-HPV status and cervical cytology have become an issue. In this study, we aimed to determine the characteristics of women with a discrepancy between histology and cytology/HR-HPV status, in terms of diagnosis, review and identification.
Material and methods: A total of 52 women, patients of the University Hospital between 2013–2015, with cytohistological or HR-HPV status discrepancy were recruited for the study and retrospectively analyzed. The cytological samples were liquid-based Pap smears, classified according to the 2001 Bethesda system. The HR-HPV status was identified using the Hybrid Capture 2 HR-HPV DNA assay. The histological samples were obtained by cervical biopsy as well as large loop excision of the transformation zone (LLETZ).
Results: A cytohistological discrepancy was demonstrated in patients with (-)cytology/HR-HPV(+), ASCUS, LSIL, ASC-H, HSIL, AGC-NOS: 17.3%, 23.07%, 26.9%, 9.5%, 17.3% and 5.7%, respectively. When the degree of atypia in cytology increases, the concurrency of cervical cytology with biopsy also increases. A positive HR-HPV co-test result (19/24, 79.1%) was observed in nearly all CIN2 ≥ (+) cases. Our study emphasizes the significance of HR-HPV testing to determine CIN2 ≥ (+) cases, even in the presence of a normal cytological result.
Conclusions: In case of cytohistological or HR-HPV discrepancies, a careful review of the HR-HPV status and the degree of cytological atypia should be performed before further intervention.
Abstract
Objectives: Discrepancies between abnormal cervical cytology or high-risk human papillomavirus (HR-HPV) status (cytolo-gy negative/HPV positive) and subsequent histological findings are a common occurrence. After using co-testing, the discrepancies between the HR-HPV status and cervical cytology have become an issue. In this study, we aimed to determine the characteristics of women with a discrepancy between histology and cytology/HR-HPV status, in terms of diagnosis, review and identification.
Material and methods: A total of 52 women, patients of the University Hospital between 2013–2015, with cytohistological or HR-HPV status discrepancy were recruited for the study and retrospectively analyzed. The cytological samples were liquid-based Pap smears, classified according to the 2001 Bethesda system. The HR-HPV status was identified using the Hybrid Capture 2 HR-HPV DNA assay. The histological samples were obtained by cervical biopsy as well as large loop excision of the transformation zone (LLETZ).
Results: A cytohistological discrepancy was demonstrated in patients with (-)cytology/HR-HPV(+), ASCUS, LSIL, ASC-H, HSIL, AGC-NOS: 17.3%, 23.07%, 26.9%, 9.5%, 17.3% and 5.7%, respectively. When the degree of atypia in cytology increases, the concurrency of cervical cytology with biopsy also increases. A positive HR-HPV co-test result (19/24, 79.1%) was observed in nearly all CIN2 ≥ (+) cases. Our study emphasizes the significance of HR-HPV testing to determine CIN2 ≥ (+) cases, even in the presence of a normal cytological result.
Conclusions: In case of cytohistological or HR-HPV discrepancies, a careful review of the HR-HPV status and the degree of cytological atypia should be performed before further intervention.
Keywords
cervical cytology, cytology/HR-HPV discrepancy, cytological atypia
About this articleTitle
Cytohistological discrepancies of cervico-vaginal smears and HPV status
Journal
Issue
Article type
Research paper
Pages
235-238
Published online
2017-05-31
Page views
1784
Article views/downloads
2681
DOI
Pubmed
Bibliographic record
Ginekol Pol 2017;88(5):235-238.
Keywords
cervical cytology
cytology/HR-HPV discrepancy
cytological atypia
Authors
İbrahim Alanbay
Mustafa Öztürk
Fahri Burçin Fıratlıgil
Kazım Emre Karaşahin
Müfit Cemal Yenen
Serkan Bodur
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