The -323P0/P10 factor VII gene polymorphism and the risk of recurrent miscarriage

Abstract

Objectives: Genetically determined disturbances in the activity of coagulation factor VII may lead to obstetric complications. The aim of the study was to evaluate the correlation between -323P0/P10 factor VII gene polymorphism and the risk of recurrent miscarriage. Material and methods: The study group consisted of 152 women with a history of ≥2 miscarriages. The control group comprised 180 women with no history of miscarriage and ≥1 pregnancy who gave birth to a healthy newborn at term. The study group was further subdivided twice into two subgroups: 114 patients with a history of 2 miscarriages and 38 subjects with a history of ≥3 miscarriages, and 123 patients with miscarriages <13 gw. and 29 with miscarriages <21 gw. Genetic analysis was performed with the use of PCR/RFLP. Results: Overrepresentation of P0/P0 genotype and lower frequency of P0/P10 genotype was noted in the study group as compared to controls (P0/P0: 80,26 vs. 76,67%, p=0,25; P0/P10: 18,42 vs. 22,78%, p=0,20). A higher presentation of P0/P0 genotype and P0 allele, lower frequency of P0/P10 genotype and P10 allele was observed in the subgroup of women with ≥3 miscarriages as compared to controls (P0/P0: 86,84 vs. 76,67%, p=0,12; P0: 93,42 vs. 88,06%, p=0,12; P0/P10:13,16 vs. 22,78%, p=0,13; P10: 11,94 vs. 6,58%, p=0,12). Conclusions: The obtained results suggest a probable protective role of -323P10 allele against the risk of miscarriage in women with ≥3 recurrent pregnancy losses.