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Histological study of intestinal goblet cells, IgA, and CD3+ lymphocyte distribution in Huang-huai white goat
Affiliations- College of Animal Science, Anhui Science and Technology University, No. 9, Donghua Road, 233100 Fengyang City, Anhui, China
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Abstract
Background: Ten healthy adult Huang-huai white goats were selected and sacrificed by jugular vein bleeding after anaesthesia to observe the distribution characteristics of the histological structure of the intestinal mucosa, goblet cells, IgA, and CD3+ lymphocytes. Materials and methods: Three sections of the duodenum, the jejunum, and the ileum were immediately collected and fixed with 4% paraformaldehyde for 72 h to prepare tissue sections. After haematoxylin and eosin, periodic acid Schiff, and immunohistochemical staining was performed, the distribution characteristics of goblet cells, IgA-positive cells, and CD3+ lymphocytes were observed. Results showed high columnar epithelial cells in the duodenum and jejunum of Huang-huai white goat and low columnar epithelial cells in the ileum mucosa. Results: Mucopolysaccharides secreted by intestinal goblet cells were mainly neutral, and the number of ileum goblet cells was significantly higher than that of the duodenum and the jejunum (p < 0.05). IgA-positive cells were distributed in the lamina propria of the duodenum, and the number of cells was significantly higher than that in the jejunum and the ileum (p < 0.01). The significant difference was found between the jejunum and the ileum (p < 0.01). The CD3+ cells in the intestinal mucosa were distributed in the lamina propria mucosae, and some of the positive cells in the jejunum were distributed between epithelial cells. CD3+ cells had the largest number in the jejunal lamina propria but had the lowest number in the ileum. Conclusions: The jejunum was significantly higher than the duodenum (p < 0.05), and the ileum was much less than the jejunum (p < 0.01).
Abstract
Background: Ten healthy adult Huang-huai white goats were selected and sacrificed by jugular vein bleeding after anaesthesia to observe the distribution characteristics of the histological structure of the intestinal mucosa, goblet cells, IgA, and CD3+ lymphocytes. Materials and methods: Three sections of the duodenum, the jejunum, and the ileum were immediately collected and fixed with 4% paraformaldehyde for 72 h to prepare tissue sections. After haematoxylin and eosin, periodic acid Schiff, and immunohistochemical staining was performed, the distribution characteristics of goblet cells, IgA-positive cells, and CD3+ lymphocytes were observed. Results showed high columnar epithelial cells in the duodenum and jejunum of Huang-huai white goat and low columnar epithelial cells in the ileum mucosa. Results: Mucopolysaccharides secreted by intestinal goblet cells were mainly neutral, and the number of ileum goblet cells was significantly higher than that of the duodenum and the jejunum (p < 0.05). IgA-positive cells were distributed in the lamina propria of the duodenum, and the number of cells was significantly higher than that in the jejunum and the ileum (p < 0.01). The significant difference was found between the jejunum and the ileum (p < 0.01). The CD3+ cells in the intestinal mucosa were distributed in the lamina propria mucosae, and some of the positive cells in the jejunum were distributed between epithelial cells. CD3+ cells had the largest number in the jejunal lamina propria but had the lowest number in the ileum. Conclusions: The jejunum was significantly higher than the duodenum (p < 0.05), and the ileum was much less than the jejunum (p < 0.01).
Keywords
goblet cells, IgA, CD3+, small intestine, Huang-huai white goat
About this articleTitle
Histological study of intestinal goblet cells, IgA, and CD3+ lymphocyte distribution in Huang-huai white goat
Journal
Issue
Article type
Original article
Pages
303-310
Published online
2019-07-17
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1940
Article views/downloads
1008
DOI
Pubmed
Bibliographic record
Folia Morphol 2020;79(2):303-310.
Keywords
goblet cells
IgA
CD3+
small intestine
Huang-huai white goat
Authors
J. Zhou
W. Zhang
W. Liu
J. Sheng
M. Li
X. Chen
R. Dong
References (30)- Brameshuber M, Kellner F, Rossboth B, et al. Monomeric TCR-CD3 Complexes Drive T-Cell Antigen Recognition. Biophys J. 2018; 114(3).
- Bukholm IK, Nesland JM, Borresen-Dale AL. Re-expression of E-cadherin, alpha-catenin and beta-catenin, but not of gamma-catenin, in metastatic tissue from breast cancer patients. J Pathol. 2000; 190(1): 15–19, doi: 10.1002/(sici)1096-9896(200001)190:1<15::aid-path489>3.0.co;2-l.
- Che CY, Yang Q. The distribution of IgA and IgG secreting cells in the gastrointestinal gract of the weaning piglets. Acta Vet Et Zootech Sin. 2010; 38: 96–100.
- Deplancke B, Gaskins HR. Microbial modulation of innate defense: goblet cells and the intestinal mucus layer. Am J Clin Nutr. 2001; 73(6): 1131S–1141S.
- Duritis I, Mugurevics A. Distribution and characterisation of goblet cells in the large intestine of ostriches during the pre- and post-hatch period. Anat Histol Embryol. 2016; 45(6): 457–462.
- Duritis I, Mugurevics A, Mancevica L. Distribution and characterization of the goblet cells in the ostrich small intestine during the pre-and posthatch period. Veterinarija ir Zootechnika. 2013; 63(85): 23–28.
- Dzharullaeva AS, Tukhvatulin AI, Erokhova AS, et al. Stimulation of dectin-1 and dectin-2 during parenteral immunization, but not mincle, induces secretory iga in intestinal mucosa. J Immunol Res. 2018; 2018: 3835720.
- Fagarasan S, Honjo T. Regulation of IgA synthesis at mucosal surfaces. Curr Opin Immunol. 2004; 16(3): 277–283.
- Gillett CE, Miles DW, Ryder K, et al. Retention of the expression of E-cadherin and catenins is associated with shorter survival in grade III ductal carcinoma of the breast. J Pathol. 2001; 193(4): 433–441.
- Gipson IK. Goblet cells of the conjunctiva: A review of recent findings. Prog Retin Eye Res. 2016; 54: 49–63.
- Gorrieri G, Scudieri P, Caci E, et al. Goblet cell hyperplasia requires high bicarbonate transport to support mucin release. Sci Rep. 2016; 6: 36016.
- Grootjans J, Hundscheid IHR, Lenaerts K, et al. Ischaemia-induced mucus barrier loss and bacterial penetration are rapidly counteracted by increased goblet cell secretory activity in human and rat colon. Gut. 2013; 62(2): 250–258.
- Ishinaga H, Kitano M, Toda M, et al. Interleukin-33 induces mucin gene expression and goblet cell hyperplasia in human nasal epithelial cells. Cytokine. 2017; 90: 60–65.
- Jung K, Saif LJ. Goblet cell depletion in small intestinal villous and crypt epithelium of conventional nursing and weaned pigs infected with porcine epidemic diarrhea virus. Res Vet Sci. 2017; 110: 12–15.
- Knoop KA, McDonald KG, McCrate S, et al. Microbial sensing by goblet cells controls immune surveillance of luminal antigens in the colon. Mucosal Immunol. 2015; 8(1): 198–210.
- Lan A, Andriamihaja M, Blouin JM, et al. High-protein diet differently modifies intestinal goblet cell characteristics and mucosal cytokine expression in ileum and colon. J Nutr Biochem. 2015; 26(1): 91–98.
- Liu H, Zhang Y. Preliminary study on distribution and type of goblet cells in digestive tract of Silurus meridionalis. Sichuan J Zoology. 2002; 21: 6–8.
- Liu Z, Chen X, Wu Q, et al. miR-125b inhibits goblet cell differentiation in allergic airway inflammation by targeting SPDEF. Eur J Pharmacol. 2016; 782: 14–20.
- Machado-Neto R, Pontin M, Nordi WM, et al. Goblet cell mucin distribution in the small intestine of newborn goat kids fed lyophilized bovine colostrum. Livestock Science. 2013; 157(1): 125–131.
- Magalhaes JG, Tattoli I, Girardin SE. The intestinal epithelial barrier: how to distinguish between the microbial flora and pathogens. Semin Immunol. 2007; 19(2): 106–115.
- McCauley HA, Guasch G. Three cheers for the goblet cell: maintaining homeostasis in mucosal epithelia. Trends Mol Med. 2015; 21(8): 492–503.
- McCauley HA, Liu CY, Attia AC, et al. TGFβ signaling inhibits goblet cell differentiation via SPDEF in conjunctival epithelium. Development. 2014; 141(23): 4628–4639.
- McDole JR, Wheeler LW, McDonald KG, et al. Goblet cells deliver luminal antigen to CD103+ dendritic cells in the small intestine. Nature. 2012; 483(7389): 345–349.
- Ngoenkam J, Schamel WW, Pongcharoen S. Selected signalling proteins recruited to the T-cell receptor-CD3 complex. Immunology. 2018; 153(1): 42–50.
- Oeser K, Schwartz C, Voehringer D. Conditional IL-4/IL-13-deficient mice reveal a critical role of innate immune cells for protective immunity against gastrointestinal helminths. Mucosal Immunol. 2015; 8(3): 672–682.
- Shirkey TW, Siggers RH, Goldade BG, et al. Effects of commensal bacteria on intestinal morphology and expression of proinflammatory cytokines in the gnotobiotic pig. Exp Biol Med (Maywood). 2006; 231(8): 1333–1345.
- Singh I. The distribution of goblet cells in the human small intestine. Cells Tissues Organs. 1971; 80(1): 68–72.
- Smith-Garvin JE, Koretzky GA, Jordan MS. T cell activation. Annu Rev Immunol. 2009; 27: 591–619.
- Stokes CR, Bailey M. Antigen handling in the gastrointestinal lamina propria. J Biotechnol. 1996; 44(1-3): 5–11.
- van der Flier LG, Clevers H. Stem cells, self-renewal, and differentiation in the intestinal epithelium. Annu Rev Physiol. 2009; 71: 241–260.
