open access

Vol 78, No 4 (2019)
Original article
Submitted: 2019-01-15
Accepted: 2019-03-01
Published online: 2019-04-03
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Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

R. A. Imam1, A. A.-E. Rizk21
·
Pubmed: 30949996
·
Folia Morphol 2019;78(4):798-808.
Affiliations
  1. Department of Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, Egypt
  2. Faculty of Medicine (Rabigh), King AbdulAziz University (KAU), +96 Rabigh, Saudi Arabia

open access

Vol 78, No 4 (2019)
ORIGINAL ARTICLES
Submitted: 2019-01-15
Accepted: 2019-03-01
Published online: 2019-04-03

Abstract

Background: Stem cells have shown promising potential to treat burn wounds. Erythropoietin was capable of promoting in vitro transdifferentiation of mesenchymal stem cells (MSCs). The aim of the study was to investigate possible role of erythropoietin-pretreated mesenchymal stem cells (EPOa/MSCs) in burn wounds healing and to evaluate its in vivo differentiation into keratinocytes. Materials and methods: Forty rats were utilised in this study divided into four groups (n = 10 for each). Control group (I), burn group (II), burn + MSCs, group (III), burn + EPOa/MSCs. 1 × 106 cells were injected locally for each 1 cm2 of burn areas. Burn areas were followed-up morphologically. After 21 days of the experiment, the rats were euthanised, skin specimens were assessed biochemically, histologically and immunohistochemically. Results: EPOa/MSCs enhanced significantly (p < 0.05) burn wound vimentin gene expression and level of interleukin (IL)-10 while decreased IL-1 and COX2 as compared to the burn group. Histologically, EPOa/MSCs improved epithelialisation despite stem cells’ differentiation into keratinocytes was rarely detected by PKH26 red fluorescence. EPOa/MSCs promoted angiogenesis as detected by significant increase in VEGF and PDGF immunoexpression as compared to burn group. Conclusions: EPOa/MSCs may improve burn wound healing, probably through anti-inflammatory, immunomodulatory and angiogenic action. However, in vivo transdifferentiation into keratinocytes was rarely detected.

Abstract

Background: Stem cells have shown promising potential to treat burn wounds. Erythropoietin was capable of promoting in vitro transdifferentiation of mesenchymal stem cells (MSCs). The aim of the study was to investigate possible role of erythropoietin-pretreated mesenchymal stem cells (EPOa/MSCs) in burn wounds healing and to evaluate its in vivo differentiation into keratinocytes. Materials and methods: Forty rats were utilised in this study divided into four groups (n = 10 for each). Control group (I), burn group (II), burn + MSCs, group (III), burn + EPOa/MSCs. 1 × 106 cells were injected locally for each 1 cm2 of burn areas. Burn areas were followed-up morphologically. After 21 days of the experiment, the rats were euthanised, skin specimens were assessed biochemically, histologically and immunohistochemically. Results: EPOa/MSCs enhanced significantly (p < 0.05) burn wound vimentin gene expression and level of interleukin (IL)-10 while decreased IL-1 and COX2 as compared to the burn group. Histologically, EPOa/MSCs improved epithelialisation despite stem cells’ differentiation into keratinocytes was rarely detected by PKH26 red fluorescence. EPOa/MSCs promoted angiogenesis as detected by significant increase in VEGF and PDGF immunoexpression as compared to burn group. Conclusions: EPOa/MSCs may improve burn wound healing, probably through anti-inflammatory, immunomodulatory and angiogenic action. However, in vivo transdifferentiation into keratinocytes was rarely detected.

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Keywords

erythropoietin, stem cells, keratinocytes, burn-rats

About this article
Title

Efficacy of erythropoietin-pretreated mesenchymal stem cells in murine burn wound healing: possible in vivo transdifferentiation into keratinocytes

Journal

Folia Morphologica

Issue

Vol 78, No 4 (2019)

Article type

Original article

Pages

798-808

Published online

2019-04-03

Page views

1685

Article views/downloads

1073

DOI

10.5603/FM.a2019.0038

Pubmed

30949996

Bibliographic record

Folia Morphol 2019;78(4):798-808.

Keywords

erythropoietin
stem cells
keratinocytes
burn-rats

Authors

R. A. Imam
A. A.-E. Rizk

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