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Comparison of type I collagens and MMP-2 proteins in temporomandibular joint of young and old mice
Affiliations- Kahramanmaras Sutcu Imam University, 46000 Kahramanmaras, Türkiye
- Department of Craniofacial Surgery, Faculty of Dentistiry , University of Gaziosmanpasa, Tokat, Turkey, Türkiye
- Department of Anatomy, Faculty of Medicine, University of Gaziosmanpasa, Tokat, Turkey, Türkiye
- Department of Biostatistics, Faculty of Medicine, University of Kahramanmaras Sutcu Imam, Kahramanmaraş, Turkey., Türkiye
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Abstract
Background: The effects of ageing on the histopathological changes of temporomandibular joint (TMJ) and the existence and age related alterations of immunochemical expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) proteins was aimed to be displayed.
Materials and methods: In this study, 14 Balb/C type white mice (50– –80 g) were included. Groups were organised as group 1 — 2-month-old young animals (n = 7) and group 2 — 18-month-old old animals (n = 7). Of the paraffin embedded tissues 4–5 μm thick sections were taken and immunohistochemical stainings of haematoxylin-eosin, type-1 collagen and MMP-2 were performed.
Results: Collagen bundles showed sagittal and oblique localisations in the young mice, which were comprised of compact collagen bundle layers positioned alternately. While collagen bundle fragmentation was observed in the disks of old mice, some disk regions showed ruptures. In the old mice a decrease in blood vessels, structural impairments and dilatation in arterioles and venules were detected. In the TMJ tissues of the young mice type I collagen and MMP-2 expressions were increased, while they were decreased in old mice. In the MMP-2 H-score evaluation young mice showed significant increase compared to the old mice.
Conclusions: Occurrence of degenerations in the collagen structure of TMJ and decimation in the matrix metalloproteases were observed with age. (Folia Morphol 2018; 77, 2: 329–334)
Abstract
Background: The effects of ageing on the histopathological changes of temporomandibular joint (TMJ) and the existence and age related alterations of immunochemical expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) proteins was aimed to be displayed.
Materials and methods: In this study, 14 Balb/C type white mice (50– –80 g) were included. Groups were organised as group 1 — 2-month-old young animals (n = 7) and group 2 — 18-month-old old animals (n = 7). Of the paraffin embedded tissues 4–5 μm thick sections were taken and immunohistochemical stainings of haematoxylin-eosin, type-1 collagen and MMP-2 were performed.
Results: Collagen bundles showed sagittal and oblique localisations in the young mice, which were comprised of compact collagen bundle layers positioned alternately. While collagen bundle fragmentation was observed in the disks of old mice, some disk regions showed ruptures. In the old mice a decrease in blood vessels, structural impairments and dilatation in arterioles and venules were detected. In the TMJ tissues of the young mice type I collagen and MMP-2 expressions were increased, while they were decreased in old mice. In the MMP-2 H-score evaluation young mice showed significant increase compared to the old mice.
Conclusions: Occurrence of degenerations in the collagen structure of TMJ and decimation in the matrix metalloproteases were observed with age. (Folia Morphol 2018; 77, 2: 329–334)
Keywords
temporomandibular joint, type I collagen, MMP-2, matrix metalloproteinase-2, immunreactivity, ageing
About this articleTitle
Comparison of type I collagens and MMP-2 proteins in temporomandibular joint of young and old mice
Journal
Issue
Article type
Original article
Pages
329-334
Published online
2017-10-17
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3058
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918
DOI
Pubmed
Bibliographic record
Folia Morphol 2018;77(2):329-334.
Keywords
temporomandibular joint
type I collagen
MMP-2
matrix metalloproteinase-2
immunreactivity
ageing
Authors
M. Demir
M.K. Tumer
M. Çiçek
M. Uysal
A. Yoldas
A. Doganer
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