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Vol 77, No 2 (2018)
Original article
Submitted: 2017-06-25
Accepted: 2017-08-30
Published online: 2017-10-17
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A comparative morphometric study of the myocardium during the postnatal development in normotensive and spontaneously hypertensive rats

A.A. Iliev1, G.N. Kotov1, B.V. Landzhov1, L.S. Jelev1, V.K. Kirkov1, D.V. Hinova-Palova1
DOI: 10.5603/FM.a2017.0094
·
Pubmed: 29064549
·
Folia Morphol 2018;77(2):253-265.
Affiliations
  1. Department of Anatomy, Histology and Embryology, Medical University of Sofia, Bulgaria

open access

Vol 77, No 2 (2018)
ORIGINAL ARTICLES
Submitted: 2017-06-25
Accepted: 2017-08-30
Published online: 2017-10-17

Abstract

Background: Differences in the size of cardiac muscle cells observed in normal and hypertrophic hearts have been assessed through different methodologies. Spontaneously hypertensive rats are often used as an experimental model of essential hypertension in humans, which allows researchers to study the relation between hypertension and cardiac hypertrophy. It has been shown that ventricular hypertrophy in mammals progresses and ventricular failure develops in the end stage of hypertrophy. The aim of the present study was to analyse a number of morphometric markers and compare them between male normotensive Wistar rats (WR) and male spontaneously hypertensive rats (SHR).

Materials and methods: The total number of male WR was 15, distributed in five age groups, each containing three animals: 2-week-old; 1-month-old; 3-month­-old; 6-month-old; 12-month-old. The male SHR were distributed in two age groups, each containing three animals: 1-month-old (young) and 6-month-old (adult).

Results: As aging progressed, both in male normotensive WR and in male SHR we noted a statistically significant increase in the morphometric parameters thickness of the free wall and the cross-sectional area of the cardiomyocytes and their nuclei and a decrease in the cardiomyocytic density in both ventricles. These changes were more pronounced and occurred at an earlier age in SHR.

Conclusions: The present study analyses in detail the alterations in the myocardium of the left and right ventricle, initiated by age-related hypertrophy, as well as hy­pertrophy induced by arterial hypertension. (Folia Morphol 2018; 77, 2: 253–265)

Abstract

Background: Differences in the size of cardiac muscle cells observed in normal and hypertrophic hearts have been assessed through different methodologies. Spontaneously hypertensive rats are often used as an experimental model of essential hypertension in humans, which allows researchers to study the relation between hypertension and cardiac hypertrophy. It has been shown that ventricular hypertrophy in mammals progresses and ventricular failure develops in the end stage of hypertrophy. The aim of the present study was to analyse a number of morphometric markers and compare them between male normotensive Wistar rats (WR) and male spontaneously hypertensive rats (SHR).

Materials and methods: The total number of male WR was 15, distributed in five age groups, each containing three animals: 2-week-old; 1-month-old; 3-month­-old; 6-month-old; 12-month-old. The male SHR were distributed in two age groups, each containing three animals: 1-month-old (young) and 6-month-old (adult).

Results: As aging progressed, both in male normotensive WR and in male SHR we noted a statistically significant increase in the morphometric parameters thickness of the free wall and the cross-sectional area of the cardiomyocytes and their nuclei and a decrease in the cardiomyocytic density in both ventricles. These changes were more pronounced and occurred at an earlier age in SHR.

Conclusions: The present study analyses in detail the alterations in the myocardium of the left and right ventricle, initiated by age-related hypertrophy, as well as hy­pertrophy induced by arterial hypertension. (Folia Morphol 2018; 77, 2: 253–265)

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Keywords

comparative quantitative study, myocardium, postnatal, normotensive rats, spontaneously hypertensive rats

About this article
Title

A comparative morphometric study of the myocardium during the postnatal development in normotensive and spontaneously hypertensive rats

Journal

Folia Morphologica

Issue

Vol 77, No 2 (2018)

Article type

Original article

Pages

253-265

Published online

2017-10-17

Page views

2178

Article views/downloads

1181

DOI

10.5603/FM.a2017.0094

Pubmed

29064549

Bibliographic record

Folia Morphol 2018;77(2):253-265.

Keywords

comparative quantitative study
myocardium
postnatal
normotensive rats
spontaneously hypertensive rats

Authors

A.A. Iliev
G.N. Kotov
B.V. Landzhov
L.S. Jelev
V.K. Kirkov
D.V. Hinova-Palova

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