open access

Ahead of Print
Original article
Submitted: 2021-07-22
Accepted: 2021-08-23
Published online: 2021-11-05
Get Citation

Axonal quantification of the white matter association fasciculi in cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus)

M. Guerrero12, I. Romero13, C. Sandoval45, A. Gaibor-Pazmiño2, A. Noroña2, V. Zurita2, M. del Sol16, N. E. Ottone716
DOI: 10.5603/FM.a2021.0116
·
Pubmed: 34750803
Affiliations
  1. Doctoral Program in Morphological Sciences, Faculty of Medicine, Universidad de La Frontera, Temuco, Chile, Chile
  2. Laboratory of Anatomy, Faculty of Medical Sciences, Universidad Central del Ecuador, Quito, Ecuador, Ecuador
  3. Faculty of Health Sciences, Department of Diagnostic Processes and Evaluation, School of Nutrition, Universidad Católica de Temuco, Temuco, Chile
  4. Departamento de Ciencias Preclínicas, Facultad de Medicina, Universidad de La Frontera, Temuco, Chile, Chile
  5. Escuela de Tecnología Médica, Facultad de Salud, Universidad Santo Tomás, Los Carreras 753, Osorno, Chile, Chile
  6. Center of Excellence in Morphological and Surgical Studies (CEMyQ), Universidad de La Frontera, Temuco, Chile, Chile
  7. Laboratory of Plastination and Anatomical Techniques, Research Centre for Dental Sciences (CICO), Department of Adults Integral Odontology, Dental School, Universidad de La Frontera, Temuco, Chile

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2021-07-22
Accepted: 2021-08-23
Published online: 2021-11-05

Abstract

Cerebral white matter consists mainly of axons surrounded by myelin sheaths, which are grouped to form association, commissural, and projection fasciculi. The aim of our work was to quantify and compare under the microscope the axons of the white matter association fasciculi in the cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus) indirectly by identification of their myelin sheaths. The samples were taken from 30 cerebral hemispheres: 10 cow, 10 pig and 10 rabbit (15 right and 15 left). They were obtained following a protocol based on the Talairach-Tournoux coordinate system for human and primate brains. The slides were stained with Luxol Fast Blue, observed by optical microscopy, and photographed at 600x. Samples were also prepared for observation in scanning transmission electron microscopy (STEM) with osmium tetroxide. The myelin sheaths/axons were counted with the ImageJ software. Statistically significant differences in the number of myelin sheaths per 410 µm2 were found in the inferior and superior longitudinal fasciculi between the left and right hemispheres of cows, with predominance of the right hemisphere; and in the inferior occipitofrontal fasciculus of the rabbit with predominance of the left hemisphere. The use of animal models for experiments in the cerebral fasciculi, especially pig, could give us a greater understanding of the behavior of demyelinating and neurodegenerative diseases in humans.

Abstract

Cerebral white matter consists mainly of axons surrounded by myelin sheaths, which are grouped to form association, commissural, and projection fasciculi. The aim of our work was to quantify and compare under the microscope the axons of the white matter association fasciculi in the cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus) indirectly by identification of their myelin sheaths. The samples were taken from 30 cerebral hemispheres: 10 cow, 10 pig and 10 rabbit (15 right and 15 left). They were obtained following a protocol based on the Talairach-Tournoux coordinate system for human and primate brains. The slides were stained with Luxol Fast Blue, observed by optical microscopy, and photographed at 600x. Samples were also prepared for observation in scanning transmission electron microscopy (STEM) with osmium tetroxide. The myelin sheaths/axons were counted with the ImageJ software. Statistically significant differences in the number of myelin sheaths per 410 µm2 were found in the inferior and superior longitudinal fasciculi between the left and right hemispheres of cows, with predominance of the right hemisphere; and in the inferior occipitofrontal fasciculus of the rabbit with predominance of the left hemisphere. The use of animal models for experiments in the cerebral fasciculi, especially pig, could give us a greater understanding of the behavior of demyelinating and neurodegenerative diseases in humans.

Get Citation

Keywords

axons, cerebral fasciculi, histological analysis, myelin sheaths, white matter

About this article
Title

Axonal quantification of the white matter association fasciculi in cerebral hemispheres of cow (Bos taurus), pig (Sus scrofa domesticus) and rabbit (Oryctolagus cuniculus)

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-11-05

DOI

10.5603/FM.a2021.0116

Pubmed

34750803

Keywords

axons
cerebral fasciculi
histological analysis
myelin sheaths
white matter

Authors

M. Guerrero
I. Romero
C. Sandoval
A. Gaibor-Pazmiño
A. Noroña
V. Zurita
M. del Sol
N. E. Ottone

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl