open access

Ahead of Print
Original article
Submitted: 2021-07-14
Accepted: 2021-10-11
Published online: 2021-10-21
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The effects of irisin on the rat thoracic aorta: histological study

R. Altaweel1, A. Shatarat1, D. Badran1, N. M. Abu Tarboush2
DOI: 10.5603/FM.a2021.0107
·
Pubmed: 34699054
Affiliations
  1. Anatomy and Histology Department, School of Medicine, The University of Jordan, Amman, Jordan
  2. Biochemistry and Physiology Department, School of Medicine, The University of Jordan, Amman, Jordan

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2021-07-14
Accepted: 2021-10-11
Published online: 2021-10-21

Abstract

Irisin, a polypeptide hormone that is released from skeletal muscle in response to exercise found to improve endothelial functions, protect against endothelial injuries and change blood pressure which also affected blood vessels. The aim of this study is to study the histological changes of the rat thoracic aorta in response to irisin injection. Thirty rats were used. Then divided into two groups; the control group without irisin injection, and the irisin injected group was subdivided into four subgroups with different irisin concentrations (20, 40 and 160 nM, respectively) twice a week for four weeks, the control group and each subgroup consisted of 6 rats each. After 4 weeks all rats were sacrificed, and the descending thoracic aorta was treated for histological evaluation. Sections were stained with Hematoxylin and Eosin (H and E) and orcein stains. Morphometric measurement included: intima-media thickness (IMT), number of elastic lamellae and number of smooth muscle cells nuclei. Histological study has shown that intraperitoneal injection of different concentrations of irisin (20, 40 and 160 nM) in rats has increased intima-media thickness, number of smooth muscle cell’s nuclei, and increase the number of elastic lamellae in media layer of the thoracic aorta in a dose dependent manner. Irisin has significantly affected the morphology of the wall of the rat thoracic aorta indicating a role for irisin in influencing the growth factors of the thoracic aorta walls and activate smooth muscle cells in the thoracic aorta layers.

Abstract

Irisin, a polypeptide hormone that is released from skeletal muscle in response to exercise found to improve endothelial functions, protect against endothelial injuries and change blood pressure which also affected blood vessels. The aim of this study is to study the histological changes of the rat thoracic aorta in response to irisin injection. Thirty rats were used. Then divided into two groups; the control group without irisin injection, and the irisin injected group was subdivided into four subgroups with different irisin concentrations (20, 40 and 160 nM, respectively) twice a week for four weeks, the control group and each subgroup consisted of 6 rats each. After 4 weeks all rats were sacrificed, and the descending thoracic aorta was treated for histological evaluation. Sections were stained with Hematoxylin and Eosin (H and E) and orcein stains. Morphometric measurement included: intima-media thickness (IMT), number of elastic lamellae and number of smooth muscle cells nuclei. Histological study has shown that intraperitoneal injection of different concentrations of irisin (20, 40 and 160 nM) in rats has increased intima-media thickness, number of smooth muscle cell’s nuclei, and increase the number of elastic lamellae in media layer of the thoracic aorta in a dose dependent manner. Irisin has significantly affected the morphology of the wall of the rat thoracic aorta indicating a role for irisin in influencing the growth factors of the thoracic aorta walls and activate smooth muscle cells in the thoracic aorta layers.

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Keywords

Irisin, hypertension, blood vessels, thoracic aorta, hematoxylin and eosin, orcein stain, morphometric measurement

About this article
Title

The effects of irisin on the rat thoracic aorta: histological study

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-10-21

DOI

10.5603/FM.a2021.0107

Pubmed

34699054

Keywords

Irisin
hypertension
blood vessels
thoracic aorta
hematoxylin and eosin
orcein stain
morphometric measurement

Authors

R. Altaweel
A. Shatarat
D. Badran
N. M. Abu Tarboush

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