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Ahead of Print
Original article
Submitted: 2021-06-02
Accepted: 2021-08-23
Published online: 2021-09-10
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The potential therapeutic efficacy of intravenous versus subconjunctival mesenchymal stem cells on experimentally ultraviolet-induced corneal injury in adult male albino rats

W. A. Nasr El-Din12, N. Nooreldin3, A. S. Essawy34
DOI: 10.5603/FM.a2021.0085
·
Pubmed: 34545564
Affiliations
  1. Department of Anatomy, College of Medicine and Medical Sciences, Arabian Gulf University, 26671 Manama, Bahrain
  2. Department of Human Anatomy and Embryology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
  3. Department of Human Anatomy and Embryology, Faculty of Medicine, Menoufia University, Shebin El-Kom, Egypt
  4. Anatomy Department, Ibn Sina National College for Medical Studies, Jeddah, Saudi Arabia

open access

Ahead of Print
ORIGINAL ARTICLES
Submitted: 2021-06-02
Accepted: 2021-08-23
Published online: 2021-09-10

Abstract

Background: The X-rays and the visible light are the main source of ultraviolet radiation (UVR). About 90% of ultraviolet B (UVB) are absorbed by the cornea which may promote corneal inflammation, oedema and damage of its epithelial layer. Bone marrow mesenchymal stem cells (BM-MSCs) have been demonstrated to ameliorate the injured corneal tissue and accelerate its wound healing. This study aimed to compare the healing effect of intravenous (IV) versus subconjunctival (SC) BM-MSCs on the rats, corneas subjected to UVB-irradiation. Materials and methods: Ten rats were used as donors for BM-MSCs and the other 40 were allocated into 4 equal groups. Group I (control group), Group II (ultraviolet irradiated group), Group III (ultraviolet irradiated + IV BM-MSCs treated group) and Group IV (ultraviolet irradiated +SC BM-MSCs treated group). Rats of all groups were euthanized after 3 weeks and the corneal specimens were processed for histopathological, immunohistochemical and electron microscopy assessment. Results: ultraviolet irradiated group showed remarkable thinning of epithelial thickness, wide partial epithelial separation, and desquamation. Neovascularization of the disorganized stroma and disrupted Descemet’s membrane. The superficial and basal epithelial cells appeared irregular and separated by wide intercellular spaces and inflammatory cells. Immunohistochemical examination showed a significant decrease in PCNA immunoreaction. In contrast, minimal changes were observed in rats treated with BM-MSCs with more improvement associated with the subconjunctival administration compared to intravenous route. Conclusions: Local SC injection of BM-MSCs has an amazing regenerative efficacy on the corneal injury compared to the systemic IV route.

Abstract

Background: The X-rays and the visible light are the main source of ultraviolet radiation (UVR). About 90% of ultraviolet B (UVB) are absorbed by the cornea which may promote corneal inflammation, oedema and damage of its epithelial layer. Bone marrow mesenchymal stem cells (BM-MSCs) have been demonstrated to ameliorate the injured corneal tissue and accelerate its wound healing. This study aimed to compare the healing effect of intravenous (IV) versus subconjunctival (SC) BM-MSCs on the rats, corneas subjected to UVB-irradiation. Materials and methods: Ten rats were used as donors for BM-MSCs and the other 40 were allocated into 4 equal groups. Group I (control group), Group II (ultraviolet irradiated group), Group III (ultraviolet irradiated + IV BM-MSCs treated group) and Group IV (ultraviolet irradiated +SC BM-MSCs treated group). Rats of all groups were euthanized after 3 weeks and the corneal specimens were processed for histopathological, immunohistochemical and electron microscopy assessment. Results: ultraviolet irradiated group showed remarkable thinning of epithelial thickness, wide partial epithelial separation, and desquamation. Neovascularization of the disorganized stroma and disrupted Descemet’s membrane. The superficial and basal epithelial cells appeared irregular and separated by wide intercellular spaces and inflammatory cells. Immunohistochemical examination showed a significant decrease in PCNA immunoreaction. In contrast, minimal changes were observed in rats treated with BM-MSCs with more improvement associated with the subconjunctival administration compared to intravenous route. Conclusions: Local SC injection of BM-MSCs has an amazing regenerative efficacy on the corneal injury compared to the systemic IV route.

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Keywords

ultraviolet radiation, corneal injury, mesenchymal stem cells, rats

About this article
Title

The potential therapeutic efficacy of intravenous versus subconjunctival mesenchymal stem cells on experimentally ultraviolet-induced corneal injury in adult male albino rats

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-09-10

DOI

10.5603/FM.a2021.0085

Pubmed

34545564

Keywords

ultraviolet radiation
corneal injury
mesenchymal stem cells
rats

Authors

W. A. Nasr El-Din
N. Nooreldin
A. S. Essawy

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