Folia Morphologica

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Original article
Published online: 2021-04-09
Submitted: 2021-02-24
Accepted: 2021-03-12
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Comparing effects of L-carnitine and sildenafil citrate on histopathologic recovery from sciatic nerve crush injury in female albino rats

O. I. Zedan1, M. A. Bashandy1
DOI: 10.5603/FM.a2021.0037
·
Pubmed: 33899209
Affiliations
  1. Anatomy and Embryology Department, Faculty of Medicine, Menoufia University, Egypt

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2021-04-09
Submitted: 2021-02-24
Accepted: 2021-03-12

Abstract

Background: The sciatic nerve is a peripheral nerve and is more vulnerable to compression with subsequent short- or long-term neuronal dysfunction. The current study was designed to elucidate the possible ameliorative effect of L-carnitine and sildenafil (SIL) on sciatic nerve crush injury. We sought to determine the effects of L-carnitine, a neuroprotective and a neuro-modulatory agent, and sildenafil citrate, a selective peripheral phosphodiesterases inhibitor on modulating neuro-degenerative changes due to sciatic nerve compression.

Materials and methods: The comparative effect of L-carnitine (at an oral dose of 20 mg/kg/day) or SIL citrate (20 mg/kg/day orally) administration for 21 days was studied in a rat model of sciatic nerve compression. Sciatic nerve sections were subjected to biochemical, histological, ultrastructure, and immunohistochemical studies to observe the effects of these treatments on neurofilament protein.

Results: The sciatic nerve crush injury group (group II) showed a significant decrease in tissue catalase (CAT), superoxide dismutase (SOD) and increase in malondhyde (MDA) as compared to control group (P < 0.01). Histological changes in the form of degenerated and vacuolated axoplasm with areas of nerve fiber loss and pyknotic nuclei were reported. The blood vessels were dilated, congested with areas of hemorrhage and mononuclear cell infiltration. Histo-morphometrically, a statistically significant reduction in the nerve fibers number, mean axon cross-sectional area, myelin sheath thickness and a significant increase in collagen fibers percentage (P < 0.05) as compared to control group. Immunohistochemically, neurofilament protein was significantly downregulated as proved by a significant reduction in mean area % of neurofilament expression. L-carnitine ameliorated the studied parameters through it`s neuroprotective effect while Sildenafil (SIL) is a selective peripheral phosphodiesterases (PDE-5) inhibitor had improved crush injury parameters but with less extent than L-carnitine.

Conclusions: These findings indicate the valuable effects of L-carnitine administration compared to that of SIL citrate in alleviating the serious debilitating effects of sciatic nerve crush injury. Our results provide a new insight into the scope of neuroprotective and, neuro-regenerative effects of L-carnitine in a sciatic nerve compression model.

Abstract

Background: The sciatic nerve is a peripheral nerve and is more vulnerable to compression with subsequent short- or long-term neuronal dysfunction. The current study was designed to elucidate the possible ameliorative effect of L-carnitine and sildenafil (SIL) on sciatic nerve crush injury. We sought to determine the effects of L-carnitine, a neuroprotective and a neuro-modulatory agent, and sildenafil citrate, a selective peripheral phosphodiesterases inhibitor on modulating neuro-degenerative changes due to sciatic nerve compression.

Materials and methods: The comparative effect of L-carnitine (at an oral dose of 20 mg/kg/day) or SIL citrate (20 mg/kg/day orally) administration for 21 days was studied in a rat model of sciatic nerve compression. Sciatic nerve sections were subjected to biochemical, histological, ultrastructure, and immunohistochemical studies to observe the effects of these treatments on neurofilament protein.

Results: The sciatic nerve crush injury group (group II) showed a significant decrease in tissue catalase (CAT), superoxide dismutase (SOD) and increase in malondhyde (MDA) as compared to control group (P < 0.01). Histological changes in the form of degenerated and vacuolated axoplasm with areas of nerve fiber loss and pyknotic nuclei were reported. The blood vessels were dilated, congested with areas of hemorrhage and mononuclear cell infiltration. Histo-morphometrically, a statistically significant reduction in the nerve fibers number, mean axon cross-sectional area, myelin sheath thickness and a significant increase in collagen fibers percentage (P < 0.05) as compared to control group. Immunohistochemically, neurofilament protein was significantly downregulated as proved by a significant reduction in mean area % of neurofilament expression. L-carnitine ameliorated the studied parameters through it`s neuroprotective effect while Sildenafil (SIL) is a selective peripheral phosphodiesterases (PDE-5) inhibitor had improved crush injury parameters but with less extent than L-carnitine.

Conclusions: These findings indicate the valuable effects of L-carnitine administration compared to that of SIL citrate in alleviating the serious debilitating effects of sciatic nerve crush injury. Our results provide a new insight into the scope of neuroprotective and, neuro-regenerative effects of L-carnitine in a sciatic nerve compression model.

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Keywords

sciatic nerve, compression, L-carnitine, sildenafil citrate, histopathology, oxidative stress, immunohistochemistry

About this article
Title

Comparing effects of L-carnitine and sildenafil citrate on histopathologic recovery from sciatic nerve crush injury in female albino rats

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-04-09

DOI

10.5603/FM.a2021.0037

Pubmed

33899209

Keywords

sciatic nerve
compression
L-carnitine
sildenafil citrate
histopathology
oxidative stress
immunohistochemistry

Authors

O. I. Zedan
M. A. Bashandy

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