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Original article
Published online: 2021-03-22
Submitted: 2020-12-10
Accepted: 2021-03-03
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Glutamate receptor antagonist suppresses the activation of nesfatin-1 neurons following refeeding or glucose administration

S. Serter Kocoglu1, C. Oy2, Z. Halk2, C. Cakir2, Z. Minbay2, O. Eyigor2
DOI: 10.5603/FM.a2021.0034
·
Pubmed: 33778937
Affiliations
  1. Department of Histology and Embryology, Balikesir University School of Medicine, Balikesir, Turkey
  2. Department of Histology and Embryology, Bursa Uludag University School of Medicine, Bursa, Turkey

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2021-03-22
Submitted: 2020-12-10
Accepted: 2021-03-03

Abstract

Background: Nesfatin-1 is a newly identified satiety peptide that has regulatory effects on food intake and glucose metabolism, and is located in the hypothalamic nuclei, including the supraoptic nucleus (SON). In this study, we have investigated the hypothesis that nesfatin-1 neurons are activated by refeeding and intraperitoneal (ip) glucose injection and that the glutamatergic system has regulatory influences on nesfatin-1 neurons in the SON. Materials and methods: The first set of experiments analyzed activation of nesfatin-1 neurons after refeeding as a physiological stimulus and the effectiveness of the glutamatergic system on this physiological stimulation. The subjects were randomly divided into three groups: fasting group, refeeding group and antagonist (CNQX+refeeding) group.  The second set of experiments analyzed activation of nesfatin-1 neurons by glucose injection as a metabolic stimulus and the effectiveness of the glutamatergic system on this metabolic stimulation. The subjects were randomly divided into three groups: saline group, glucose group and antagonist (CNQX+glucose) group. Results: Refeeding significantly increased the number of activated nesfatin-1 neurons by approximately 66%, and intraperitoneal glucose injection activated these neurons by about 55%, compared to the fasting and saline controls. The injections of glutamate antagonist (CNQX) greatly decreased the number of activated nesfatin-1 neurons. Conclusions: This study suggested that nesfatin-1 neurons were activated by peripheral and/or metabolic signals and that this effect was mediated through the glutamatergic system.

Abstract

Background: Nesfatin-1 is a newly identified satiety peptide that has regulatory effects on food intake and glucose metabolism, and is located in the hypothalamic nuclei, including the supraoptic nucleus (SON). In this study, we have investigated the hypothesis that nesfatin-1 neurons are activated by refeeding and intraperitoneal (ip) glucose injection and that the glutamatergic system has regulatory influences on nesfatin-1 neurons in the SON. Materials and methods: The first set of experiments analyzed activation of nesfatin-1 neurons after refeeding as a physiological stimulus and the effectiveness of the glutamatergic system on this physiological stimulation. The subjects were randomly divided into three groups: fasting group, refeeding group and antagonist (CNQX+refeeding) group.  The second set of experiments analyzed activation of nesfatin-1 neurons by glucose injection as a metabolic stimulus and the effectiveness of the glutamatergic system on this metabolic stimulation. The subjects were randomly divided into three groups: saline group, glucose group and antagonist (CNQX+glucose) group. Results: Refeeding significantly increased the number of activated nesfatin-1 neurons by approximately 66%, and intraperitoneal glucose injection activated these neurons by about 55%, compared to the fasting and saline controls. The injections of glutamate antagonist (CNQX) greatly decreased the number of activated nesfatin-1 neurons. Conclusions: This study suggested that nesfatin-1 neurons were activated by peripheral and/or metabolic signals and that this effect was mediated through the glutamatergic system.

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Keywords

CNQX, glucose, glutamate, nesfatin-1, refeeding

About this article
Title

Glutamate receptor antagonist suppresses the activation of nesfatin-1 neurons following refeeding or glucose administration

Journal

Folia Morphologica

Issue

Ahead of Print

Article type

Original article

Published online

2021-03-22

DOI

10.5603/FM.a2021.0034

Pubmed

33778937

Keywords

CNQX
glucose
glutamate
nesfatin-1
refeeding

Authors

S. Serter Kocoglu
C. Oy
Z. Halk
C. Cakir
Z. Minbay
O. Eyigor

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