open access

Vol 79, No 3 (2020)
ORIGINAL ARTICLES
Published online: 2019-11-12
Submitted: 2019-09-30
Accepted: 2019-10-16
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Aqueous anise extract alleviated the pancreatic changes in streptozotocin-induced diabetic rat model via modulation of hyperglycaemia, oxidative stress, apoptosis and autophagy: a biochemical, histological and immunohistochemical study

M. A. Faried, A. E.S. El-Mehi
DOI: 10.5603/FM.a2019.0117
·
Pubmed: 31724151
·
Folia Morphol 2020;79(3):489-502.

open access

Vol 79, No 3 (2020)
ORIGINAL ARTICLES
Published online: 2019-11-12
Submitted: 2019-09-30
Accepted: 2019-10-16

Abstract

Background: The present study aimed to investigate, for the first time to the best of our knowledge, the effect of aqueous anise extract on the pancreatic damage in the streptozotocin (STZ)-induced diabetic rat model with referral to some of its underlying mechanisms.

Materials and methods: Forty adult male albino rats were divided equally into four groups; control, anise extract treated (500 mg/kg orally once daily), diabetic control group (STZ 50 mg/kg once intraperitoneally) and diabetic group treated with anise extract. At the end of experiment (7 weeks), body weight, blood glucose and serum amylase levels were assessed. Pancreatic tissues were subjected to biochemical, histological (light and electron microscopic), and immunohistochemical studies.

Results: The diabetic group exhibited significant decrease in body weight and increase in blood glucose and serum amylase levels. Marked degenerative changes affecting both b-cells and acinar cells of the pancreas in the form of a significant decrease in islet’s perimeter, vacuolated cytoplasm, pyknotic nuclei, depletion of zymogen granules, dilated congested blood vessels and degenerated organelles were reported. Hyperglycaemia-induced oxidative stress with subsequent upregulation of caspase 3 and beclin 1 immunoreaction were suggested to be implicated in diabetes mellitus pathogenesis. Anise extract ameliorated the all examined parameters via its hypoglycaemic and antioxidant properties with subsequent downregulation of apoptosis and autophagy.

Conclusions: Anise extract can be a promising agent in the control of diabetes mellitus for further clinical trials.

Abstract

Background: The present study aimed to investigate, for the first time to the best of our knowledge, the effect of aqueous anise extract on the pancreatic damage in the streptozotocin (STZ)-induced diabetic rat model with referral to some of its underlying mechanisms.

Materials and methods: Forty adult male albino rats were divided equally into four groups; control, anise extract treated (500 mg/kg orally once daily), diabetic control group (STZ 50 mg/kg once intraperitoneally) and diabetic group treated with anise extract. At the end of experiment (7 weeks), body weight, blood glucose and serum amylase levels were assessed. Pancreatic tissues were subjected to biochemical, histological (light and electron microscopic), and immunohistochemical studies.

Results: The diabetic group exhibited significant decrease in body weight and increase in blood glucose and serum amylase levels. Marked degenerative changes affecting both b-cells and acinar cells of the pancreas in the form of a significant decrease in islet’s perimeter, vacuolated cytoplasm, pyknotic nuclei, depletion of zymogen granules, dilated congested blood vessels and degenerated organelles were reported. Hyperglycaemia-induced oxidative stress with subsequent upregulation of caspase 3 and beclin 1 immunoreaction were suggested to be implicated in diabetes mellitus pathogenesis. Anise extract ameliorated the all examined parameters via its hypoglycaemic and antioxidant properties with subsequent downregulation of apoptosis and autophagy.

Conclusions: Anise extract can be a promising agent in the control of diabetes mellitus for further clinical trials.

Get Citation

Keywords

anise; diabetes; pancreas; autophagy; apoptosis; oxidative stress; histopathology

About this article
Title

Aqueous anise extract alleviated the pancreatic changes in streptozotocin-induced diabetic rat model via modulation of hyperglycaemia, oxidative stress, apoptosis and autophagy: a biochemical, histological and immunohistochemical study

Journal

Folia Morphologica

Issue

Vol 79, No 3 (2020)

Pages

489-502

Published online

2019-11-12

DOI

10.5603/FM.a2019.0117

Pubmed

31724151

Bibliographic record

Folia Morphol 2020;79(3):489-502.

Keywords

anise
diabetes
pancreas
autophagy
apoptosis
oxidative stress
histopathology

Authors

M. A. Faried
A. E.S. El-Mehi

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