open access

Vol 79, No 3 (2020)
ORIGINAL ARTICLES
Published online: 2019-11-12
Submitted: 2019-09-02
Accepted: 2019-10-22
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The structural characteristics of photoageing in mice caused by the effects of ultraviolet A radiation

S. Savic, S. Smiljic, S. Lestarevic, A. Ilic, M. Mijovic, P. Mandic, B. Djerkovic
DOI: 10.5603/FM.a2019.0119
·
Pubmed: 31724149
·
Folia Morphol 2020;79(3):548-556.

open access

Vol 79, No 3 (2020)
ORIGINAL ARTICLES
Published online: 2019-11-12
Submitted: 2019-09-02
Accepted: 2019-10-22

Abstract

Background: Due to its deep penetration into the dermis, ultraviolet A (UVA) radiation is considered a primary factor in skin photoageing. The aim of this study is to use a qualitative and quantitative analysis to determine the structural parameters of skin photoageing in mice exposed to UVA radiation, with or without the application of a photoprotective cream. Materials and methods: The experiment consisted of the radiation of female BALBc mice in a solarium by UVA rays, up to total dosages of 7800 J/cm2 and 12500 J/cm2. A total of 78 animals were divided into four experimental and two control groups. All animals were shaved and the animals in two experimental groups were treated with a photoprotective cream half an hour before exposure. The samples of the treated skin were stained with haematoxylin eosin and Van-Gieson staining methods. All measurements, except for the presence of dyskeratosis, were taken using ImageJ 150i software. Results: In the study, the signs of skin photoageing were more evident in untreated groups of animals. Dyskeratosis was more frequent in both of the untreated groups of animals (p = 0.004 and p = 0.003). The lowest values of epidermal thickness (13.8 ± 2.6 μm and 12.7 ± 2.3 μm) were present in both of the untreated groups of animals (p < 0.001 and p < 0.001). The highest values of stratum corneum thickness (34.3 ± 8.5 μm) were observed in the untreated, shorter radiated group of animals (p < 0.001) which was irradiated for the shortest period of time. Beside the control groups, the highest length of dermo-epidermal junction was recorded in the group of treated, longer radiated animals (1467.6 ± 94.6 μm; p = 0.373). The lowest values of dermal thickness (115.9 ± 10.5 μm and 134.8 ± 21.8 μm) and volumetric density of the collagen fibres (31.92 ± 3.19% and 29.40 ± 4.54%) were present in both untreated groups of animals (p < 0.001, p < 0.001, p = 0.035). Conclusions: Skin photoageing was most pronounced in the groups of animals irradiated without the application of photoprotective cream.

Abstract

Background: Due to its deep penetration into the dermis, ultraviolet A (UVA) radiation is considered a primary factor in skin photoageing. The aim of this study is to use a qualitative and quantitative analysis to determine the structural parameters of skin photoageing in mice exposed to UVA radiation, with or without the application of a photoprotective cream. Materials and methods: The experiment consisted of the radiation of female BALBc mice in a solarium by UVA rays, up to total dosages of 7800 J/cm2 and 12500 J/cm2. A total of 78 animals were divided into four experimental and two control groups. All animals were shaved and the animals in two experimental groups were treated with a photoprotective cream half an hour before exposure. The samples of the treated skin were stained with haematoxylin eosin and Van-Gieson staining methods. All measurements, except for the presence of dyskeratosis, were taken using ImageJ 150i software. Results: In the study, the signs of skin photoageing were more evident in untreated groups of animals. Dyskeratosis was more frequent in both of the untreated groups of animals (p = 0.004 and p = 0.003). The lowest values of epidermal thickness (13.8 ± 2.6 μm and 12.7 ± 2.3 μm) were present in both of the untreated groups of animals (p < 0.001 and p < 0.001). The highest values of stratum corneum thickness (34.3 ± 8.5 μm) were observed in the untreated, shorter radiated group of animals (p < 0.001) which was irradiated for the shortest period of time. Beside the control groups, the highest length of dermo-epidermal junction was recorded in the group of treated, longer radiated animals (1467.6 ± 94.6 μm; p = 0.373). The lowest values of dermal thickness (115.9 ± 10.5 μm and 134.8 ± 21.8 μm) and volumetric density of the collagen fibres (31.92 ± 3.19% and 29.40 ± 4.54%) were present in both untreated groups of animals (p < 0.001, p < 0.001, p = 0.035). Conclusions: Skin photoageing was most pronounced in the groups of animals irradiated without the application of photoprotective cream.

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Keywords

photoageing; photoprotection; irradiation; ImageJ; mice

About this article
Title

The structural characteristics of photoageing in mice caused by the effects of ultraviolet A radiation

Journal

Folia Morphologica

Issue

Vol 79, No 3 (2020)

Pages

548-556

Published online

2019-11-12

DOI

10.5603/FM.a2019.0119

Pubmed

31724149

Bibliographic record

Folia Morphol 2020;79(3):548-556.

Keywords

photoageing
photoprotection
irradiation
ImageJ
mice

Authors

S. Savic
S. Smiljic
S. Lestarevic
A. Ilic
M. Mijovic
P. Mandic
B. Djerkovic

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