open access

Vol 79, No 2 (2020)
ORIGINAL ARTICLES
Published online: 2019-08-14
Submitted: 2019-07-10
Accepted: 2019-07-29
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Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: a biochemical and histopathological study

A. Oğuz, A. Böyük, A. Ekinci, U. Alabalik, A. Türkoğlu, M. C. Tuncer, A. Ekingen, E. Deveci, B. Gültürk, U. Aday
DOI: 10.5603/FM.a2019.0087
·
Pubmed: 31436305
·
Folia Morphol 2020;79(2):288-295.

open access

Vol 79, No 2 (2020)
ORIGINAL ARTICLES
Published online: 2019-08-14
Submitted: 2019-07-10
Accepted: 2019-07-29

Abstract

Background: The aim of the study was to investigate the protective effects of rosmarinic acid in rats exposed to hepatic ischaemia/reperfusion (I/R) injury.

Materials and methods: Thirty-two rats were randomly classified into four groups of 8 rats each: laparotomy without medication, rosmarinic acid (dose of 50 mg/kg via oral gavage) followed by laparotomy, laparotomy followed by hepatic I/R, and hepatic I/R with rosmarinic acid. Serum aspartate aminotransferase, alaninę aminotransferase, and malondialdehyde levels and total oxidant activity and total antioxidant capacity levels of the liver, lung, and kidney were assessed. The histopathologic assessment was also performed.

Results: Rosmarinic acid significantly reduced liver function test parameters and decreased oxidative stress and abnormal histopathologic findings in the liver. The oxidative stress in the lung significantly increased in the I/R group but significantly decreased in the I/R + rosmarinic acid group due to the addition of rosmarinic acid. Rosmarinic acid led to no reduction in oxidative stress in kidney following hepatic I/R injury. There were no statistically significant differences among the groups regarding histopathologic changes in kidney and lung sections.

Conclusions: Rosmarinic acid has antioxidant properties and is an effective hepatoprotective agent. However, although rosmarinic acid provides useful effects in the lung by increasing antioxidant capacity and reducing oxidative stress after I/R injury, it does not ameliorate histopathologic changes. These findings suggest that rosmarinic acid is likely to provide favourable outcomes in the treatment of hepatic I/R injury.

Abstract

Background: The aim of the study was to investigate the protective effects of rosmarinic acid in rats exposed to hepatic ischaemia/reperfusion (I/R) injury.

Materials and methods: Thirty-two rats were randomly classified into four groups of 8 rats each: laparotomy without medication, rosmarinic acid (dose of 50 mg/kg via oral gavage) followed by laparotomy, laparotomy followed by hepatic I/R, and hepatic I/R with rosmarinic acid. Serum aspartate aminotransferase, alaninę aminotransferase, and malondialdehyde levels and total oxidant activity and total antioxidant capacity levels of the liver, lung, and kidney were assessed. The histopathologic assessment was also performed.

Results: Rosmarinic acid significantly reduced liver function test parameters and decreased oxidative stress and abnormal histopathologic findings in the liver. The oxidative stress in the lung significantly increased in the I/R group but significantly decreased in the I/R + rosmarinic acid group due to the addition of rosmarinic acid. Rosmarinic acid led to no reduction in oxidative stress in kidney following hepatic I/R injury. There were no statistically significant differences among the groups regarding histopathologic changes in kidney and lung sections.

Conclusions: Rosmarinic acid has antioxidant properties and is an effective hepatoprotective agent. However, although rosmarinic acid provides useful effects in the lung by increasing antioxidant capacity and reducing oxidative stress after I/R injury, it does not ameliorate histopathologic changes. These findings suggest that rosmarinic acid is likely to provide favourable outcomes in the treatment of hepatic I/R injury.

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Keywords

hepatic ischaemia-reperfusion, rosmarinic acid, oxidant and antioxidant capacity, liver, kidney, lung

About this article
Title

Investigation of antioxidant effects of rosmarinic acid on liver, lung and kidney in rats: a biochemical and histopathological study

Journal

Folia Morphologica

Issue

Vol 79, No 2 (2020)

Pages

288-295

Published online

2019-08-14

DOI

10.5603/FM.a2019.0087

Pubmed

31436305

Bibliographic record

Folia Morphol 2020;79(2):288-295.

Keywords

hepatic ischaemia-reperfusion
rosmarinic acid
oxidant and antioxidant capacity
liver
kidney
lung

Authors

A. Oğuz
A. Böyük
A. Ekinci
U. Alabalik
A. Türkoğlu
M. C. Tuncer
A. Ekingen
E. Deveci
B. Gültürk
U. Aday

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