open access

Vol 79, No 1 (2020)
Original article
Submitted: 2019-03-13
Accepted: 2019-04-07
Published online: 2019-04-11
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Reconstruction of lymphatic vessels in the mouse tail after cupping therapy

F.-W. Meng1, Z.-L. Gao1, L. Li1, L.-L. Jie1, P.-F. Yang1, Z. Liang2, Y.-W. Gao3, W.-H. Liu1
·
Pubmed: 30993665
·
Folia Morphol 2020;79(1):98-104.
Affiliations
  1. Department of Anatomy and Physiology, Shandong College of Traditional Chinese Medicine, Yantai, China
  2. Biological Science and Technology Institute, Weifang Medical University, Weifang, China
  3. Department of Breast and Thyroid Surgery, Yantai Affiliated Hospital, Binzhou Medical University, Yantai, China

open access

Vol 79, No 1 (2020)
ORIGINAL ARTICLES
Submitted: 2019-03-13
Accepted: 2019-04-07
Published online: 2019-04-11

Abstract

Background: The aim of the study was to investigate the regulatory mechanism of local lymphatic reconstruction after cupping therapy in a mouse model.

Materials and methods: The lymphatic reconstruction process in the mouse tail after cupping therapy as well as the expression levels of the vascular endothelial identification molecule CD34, prospero homeobox protein 1 (PROX1), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) were investigated for a duration of 4 days through immunohistochemistry experiments.

Results: On day 1 after cupping therapy, the CD34+ and LYVE-1+ cell densities were significantly increased, and the formed CD34+LYVE-1+ tubular structure started to express PROX1. This was followed by a decrease in both the CD34+ and LYVE-1+ stem cell densities to basal levels on the second day after cupping therapy. Both the CD34+ and LYVE-1+ cell densities subsequently increased again on the third day after cupping therapy. The increase in the LYVE-1+ density was accompanied by tubular structure formation, which is characteristic of lymphangiogenesis. In addition, the colocalisation of CD34+ and LYVE-1+ cells by immunohistochemistry suggests that the CD34+ stem cells differentiated into new lymphatic endothelial cells.

Conclusions: Our findings indicate that the mechanism underlying the therapeutic effect of cupping therapy involves upregulation of vascular and lymphatic endothelial markers (CD34+, LYVE-1+, and CD34+LYVE-1+) in local tissues, which in turn promotes local new lymphatic vessel formation through the expression of PROX1.

Abstract

Background: The aim of the study was to investigate the regulatory mechanism of local lymphatic reconstruction after cupping therapy in a mouse model.

Materials and methods: The lymphatic reconstruction process in the mouse tail after cupping therapy as well as the expression levels of the vascular endothelial identification molecule CD34, prospero homeobox protein 1 (PROX1), and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE-1) were investigated for a duration of 4 days through immunohistochemistry experiments.

Results: On day 1 after cupping therapy, the CD34+ and LYVE-1+ cell densities were significantly increased, and the formed CD34+LYVE-1+ tubular structure started to express PROX1. This was followed by a decrease in both the CD34+ and LYVE-1+ stem cell densities to basal levels on the second day after cupping therapy. Both the CD34+ and LYVE-1+ cell densities subsequently increased again on the third day after cupping therapy. The increase in the LYVE-1+ density was accompanied by tubular structure formation, which is characteristic of lymphangiogenesis. In addition, the colocalisation of CD34+ and LYVE-1+ cells by immunohistochemistry suggests that the CD34+ stem cells differentiated into new lymphatic endothelial cells.

Conclusions: Our findings indicate that the mechanism underlying the therapeutic effect of cupping therapy involves upregulation of vascular and lymphatic endothelial markers (CD34+, LYVE-1+, and CD34+LYVE-1+) in local tissues, which in turn promotes local new lymphatic vessel formation through the expression of PROX1.

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Keywords

cupping, lymphatic regeneration, mice, LYVE-1, PROX1, CD34

About this article
Title

Reconstruction of lymphatic vessels in the mouse tail after cupping therapy

Journal

Folia Morphologica

Issue

Vol 79, No 1 (2020)

Article type

Original article

Pages

98-104

Published online

2019-04-11

Page views

2952

Article views/downloads

917

DOI

10.5603/FM.a2019.0044

Pubmed

30993665

Bibliographic record

Folia Morphol 2020;79(1):98-104.

Keywords

cupping
lymphatic regeneration
mice
LYVE-1
PROX1
CD34

Authors

F.-W. Meng
Z.-L. Gao
L. Li
L.-L. Jie
P.-F. Yang
Z. Liang
Y.-W. Gao
W.-H. Liu

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