Vol 78, No 3 (2019)
Original article
Published online: 2019-01-03

open access

Page views 1352
Article views/downloads 1170
Get Citation

Connect on Social Media

Connect on Social Media

Quality and quantity comparison study of corrosion casts of bovine testis made using two synthetic kits: Plastogen G and Batson no. 17

M. Polguj1, P. Kiciński1, Z. Nowicka2, K. Barszcz3, O. Szaluś-Jordanow3, M. Topol4
Pubmed: 30644083
Folia Morphol 2019;78(3):487-493.

Abstract

Background: Although corrosion casting has been implemented for almost five centuries, the choice of resin has a strong influence on the effectiveness of casting vessel formations. Our aim was to compare quality and quantity features of two groups of corrosion casts made using two kits: Plastogen G resin and Batson no. 17 resin.

Materials and methods: Thirty corrosion casts were made of testicular arterial vessels (15 made using Plastogen G and 15 made using Batson no. 17) and their shape, colour, fragility and flexibility were evaluated. The following parameters were measured: maximal width of corrosion cast of testis, maximal length of centripetal and centrifugal arteries, diameter of testicular artery and its terminal branches. Based on these measurements, five indexes were calculated (Iq1–Iq5).

Results: Generally both groups of corrosion casts demonstrated similar attributes. Only the rami tunicales minores displayed higher fragility and lower flexibility in the Plastogen G group than the Batson no. 17 group. The only observed quantitative difference was that Iq3 was significantly lower in the Plastogen G group (0.71 ± 0.01) than in Batson no. 17 group (0.79 ± 0.01; p = 0.0092).

Conclusions: The two corrosion cast groups displayed similar qualitative and quantitative attributes. Batson no. 17 appears to be a good resin for three-dimensional visualisation of extra- and intratesticular arterial vessels.

Article available in PDF format

View PDF Download PDF file

References

  1. Barszcz K, Kupczyńska M, Janczyk P, et al. Venous drainage of the heart in the domestic cat. Med Weter. 2016; 72: 186–190.
  2. Barszcz K, Kupczyńska M, Klećkowska-Nawrot J, et al. Arterial coronary circulation in cats. Med Weter. 2014; 70: 373–377.
  3. Barszcz K, Kupczyńska M, Polguj M, et al. Morphometry of the coronary ostia and the structure of coronary arteries in the shorthair domestic cat. PLoS One. 2017; 12(10): e0186177.
  4. Doomernik DE, Kruse RR, Reijnen MM, et al. A comparative study of vascular injection fluids in fresh-frozen and embalmed human cadaver forearms. J Anat. 2016; 229(4): 582–590.
  5. Giuvărăşteanu I. Scanning electron microscopy of vascular corrosion casts--standard method for studying microvessels. Rom J Morphol Embryol. 2007; 48(3): 257–261.
  6. Goetzen B. [The use of latex geon 265 in anatomic studies of blood vessels and ducts]. Folia Morphol. 1966; 25(1): 147–151.
  7. Gutschiebauch A. Der Hoden der Haussäugetiere und seine Hüllen in biologischer und artdiagnostischer Hinsicht. Z Anat Ew Gesch. 1936; 105(4): 433–458.
  8. Hofmann R. Die Gefäßarchitektur des Bullenhodens, zugleich ein Versuch ihrer funktionellen Deutung. Zentralbl Veterinarmed. 1960; 7(1): 59–93.
  9. Hossler FE, Douglas JE, Verghese A, et al. Microvascular architecture of the elastase emphysemic hamster lung. J Electron Microsc Tech. 1991; 19(4): 406–418.
  10. Hossler FE, Douglas JE. Vascular corrosion casting: review of advantages and limitations in the application of some simple quantitative methods. Microsc Microanal. 2001; 7(3): 253–264.
  11. Jedrzejewski KS, Cendrowska I, Okraszewska E, et al. Comparative study of the intratesticular vascular rete in men and certain animals. Folia Morphol. 1996; 55(4): 303–305.
  12. Konerding MA. Scanning electron microscopy of corrosion casting in medicine. Scanning Microsc. 1991; 5(3): 851–865.
  13. König HE, Liebich HG. Veterinary anatomy of domestic mammals. Schattauer Verlag. 2007: Stuttgart.
  14. Kupczyńska M, Barszcz K, Olbrych K, et al. Coronary arteries of the European bison (Bison bonasus). Acta Vet Scand. 2015; 57: 82.
  15. Murakami T, Hitomi S, Ohtsuka A, et al. Pancreatic insulo-acinar portal systems in humans, rats, and some other mammals: Scanning electron microscopy of vascular casts. Microsc Res Tech. 1997; 37(5-6): 478–488, doi: 10.1002/(sici)1097-0029(19970601)37:5/6<478::aid-jemt10>3.0.co;2-n.
  16. Ohtsuka A. Microvascular architecture of the pampiniform plexus-testicular artery system in the rat: a scanning electron microscope study of corrosion casts. Am J Anat. 1984; 169(3): 285–293.
  17. Okraszewska E, Cendrowska I, Jedrzejewski KS. The intratesticular lymphatic network in men, bulls and rams. Folia Morphol. 1996; 55(4): 401–402.
  18. Polguj M, Jędrzejewski KS, Bolanowski W. Comparison of blood vessels complex of human and bovine masculine gonad. Folia Morphol. 2008; 67(3): 179–185.
  19. Polguj M, Jedrzejewski KS, Dyl L, et al. Topographic and morphometric comparison study of the terminal part of human and bovine testicular arteries. Folia Morphol. 2009; 68(4): 271–276.
  20. Polguj M, Sopiński M, Jędrzejewski K, et al. Angioarchitecture of the bovine tunica albuginea vascular complex--a corrosive and histological study. Res Vet Sci. 2011; 91(2): 181–187.
  21. Polguj M, Wysiadecki G, Podgórski M, et al. Morphological variations of intra-testicular arterial vasculature in bovine testis--a corrosion casting study. BMC Vet Res. 2015; 11: 263.
  22. Polguj M, Jȩdrzejewski KS, Topol M. Angioarchitecture of the bovine spermatic cord. J Morphol. 2011; 272(4): 497–502.
  23. Skowroński A, Jedrzejewski K. The human testicular artery and the pampiniform plexus--where is the connection? Folia Morphol. 2003; 62(3): 201–204.
  24. Tompsett DH. Anatomical techniques. E & S Livingstone, Edinburgh. 1970: 93–95.