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REVIEW ARTICLES
Published online: 2018-12-05
Submitted: 2018-08-14
Accepted: 2018-09-14
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Klingler’s method of brain dissection: review of the technique including its usefulness in practical neuroanatomy teaching, neurosurgery and neuroimaging

Grzegorz Wysiadecki, Edward Clarke, Michał Polguj, Robert Haładaj, Andrzej Żytkowski, Mirosław Topol
DOI: 10.5603/FM.a2018.0113
·
Pubmed: 30536356

open access

Ahead of Print
REVIEW ARTICLES
Published online: 2018-12-05
Submitted: 2018-08-14
Accepted: 2018-09-14

Abstract

Klingler’s technique was discovered in the 1930s. It is a modified method of brain fixation and dissection, based on freezing and thawing of the brain tissue, subsequent peeling away of white matter fibers and the gradual exposure of white matter tracts. The added value of this technique is that it is carried out in a stratigraphic manner; This fact makes it an invaluable tool for an in-depth understanding of the complex anatomical organization of the cerebral hemispheres.

The purpose of this paper is to provide a review of Klingler’s method while taking into account the original description of the technique and its value for medical training. The historical background, the concise outline of white matter organization, as well as our own experience in using this procedure for research and teaching activities were also included.

The fiber dissection technique may still be considered an excellent complementary research tool for neuroanatomical studies. Numerous detailed observations about the white matter topography and spatial organization have been recently made by applying this method. Using this technique may also improve understanding of the three-dimensional intrinsic structure of the brain, which is particularly important both in under- and postgraduate training in the field of neuroanatomy.

Abstract

Klingler’s technique was discovered in the 1930s. It is a modified method of brain fixation and dissection, based on freezing and thawing of the brain tissue, subsequent peeling away of white matter fibers and the gradual exposure of white matter tracts. The added value of this technique is that it is carried out in a stratigraphic manner; This fact makes it an invaluable tool for an in-depth understanding of the complex anatomical organization of the cerebral hemispheres.

The purpose of this paper is to provide a review of Klingler’s method while taking into account the original description of the technique and its value for medical training. The historical background, the concise outline of white matter organization, as well as our own experience in using this procedure for research and teaching activities were also included.

The fiber dissection technique may still be considered an excellent complementary research tool for neuroanatomical studies. Numerous detailed observations about the white matter topography and spatial organization have been recently made by applying this method. Using this technique may also improve understanding of the three-dimensional intrinsic structure of the brain, which is particularly important both in under- and postgraduate training in the field of neuroanatomy.

Get Citation

Keywords

fiber dissection technique; Klingler' s technique; neuroanatomy; white matter; tractography

About this article
Title

Klingler’s method of brain dissection: review of the technique including its usefulness in practical neuroanatomy teaching, neurosurgery and neuroimaging

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2018-12-05

DOI

10.5603/FM.a2018.0113

Pubmed

30536356

Keywords

fiber dissection technique
Klingler's technique
neuroanatomy
white matter
tractography

Authors

Grzegorz Wysiadecki
Edward Clarke
Michał Polguj
Robert Haładaj
Andrzej Żytkowski
Mirosław Topol

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