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ORIGINAL ARTICLES
Published online: 2018-08-24
Submitted: 2018-06-09
Accepted: 2018-06-26
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Aging changes in the retina of male albino rat: histological, ultrastructural and immunohistochemical study

Mohamed Mohamed, Ehab Abdel Aziz El-Shaarawy, Magdy Youakim, Doaa Shuaib, Mai Ahmed
DOI: 10.5603/FM.a2018.0075
·
Pubmed: 30155876

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2018-08-24
Submitted: 2018-06-09
Accepted: 2018-06-26

Abstract

Background: Degenerative changes caused by aging may affect the eye specially the retina. Such changes occurs as a part of normal physiological process and may be irreversible.

Aim: The aim of the study was to demonstrate the influence of aging on the morphology of the retina to provide a basis to explain the pathogenesis of age-associated decline in visual acuity, scotopic and photopic sensitivity.

Materials and methods: Forty male albino rats were used and divided into four age groups; group I: age of cortical maturity, group II: middle-aged, group III: aged group and group IV: senile group. The rats were sacrificed, the eye balls were enucleated. Intra-vitreal injections of formalin for Hx&E and immunohistochemical sections, glutaraldehyde for toluidine blue semithin and E/M ultra-thin sections was performed. Measurements and quantitative histomorphometric estimation of the layers of the retina were done.

Results: Light microscopic examination revealed age-dependent attenuation of photoreceptor striations. Aged and senile groups presented pyknotic, widely-spaced nuclei of the outer nuclear layer. The inner nuclear layer was thinned out to two or three cellular rows. Retinal capillaries showed progressive dilatation and congestion. Statistical analysis proved significant thinning of the retina with variable degrees of thinning of the constituting layers. Decreased arborization with age was confirmed with quantification of synaptophysin-immunostained sections. Glial fibrillary acidic protein immunostaining revealed the picture of reactive gliosis. On the ultrastructural level, the retinal pigment epithelium exhibited major alterations with aging. Numerous phagosomes, lipofuscin and melanolipofusin granules appeared within the cells, together with exaggerated basal infoldings. The photoreceptor nuclei became degenerated and the perinuclear space was widened. 

Conclusion: Rat retinae clearly undergo age-related morphological changes such changes provide a cellular base for explanation of decreased vision in humans with aging other than reflection errors. Effect of aging was not only qualitative, but also quantitative.

Abstract

Background: Degenerative changes caused by aging may affect the eye specially the retina. Such changes occurs as a part of normal physiological process and may be irreversible.

Aim: The aim of the study was to demonstrate the influence of aging on the morphology of the retina to provide a basis to explain the pathogenesis of age-associated decline in visual acuity, scotopic and photopic sensitivity.

Materials and methods: Forty male albino rats were used and divided into four age groups; group I: age of cortical maturity, group II: middle-aged, group III: aged group and group IV: senile group. The rats were sacrificed, the eye balls were enucleated. Intra-vitreal injections of formalin for Hx&E and immunohistochemical sections, glutaraldehyde for toluidine blue semithin and E/M ultra-thin sections was performed. Measurements and quantitative histomorphometric estimation of the layers of the retina were done.

Results: Light microscopic examination revealed age-dependent attenuation of photoreceptor striations. Aged and senile groups presented pyknotic, widely-spaced nuclei of the outer nuclear layer. The inner nuclear layer was thinned out to two or three cellular rows. Retinal capillaries showed progressive dilatation and congestion. Statistical analysis proved significant thinning of the retina with variable degrees of thinning of the constituting layers. Decreased arborization with age was confirmed with quantification of synaptophysin-immunostained sections. Glial fibrillary acidic protein immunostaining revealed the picture of reactive gliosis. On the ultrastructural level, the retinal pigment epithelium exhibited major alterations with aging. Numerous phagosomes, lipofuscin and melanolipofusin granules appeared within the cells, together with exaggerated basal infoldings. The photoreceptor nuclei became degenerated and the perinuclear space was widened. 

Conclusion: Rat retinae clearly undergo age-related morphological changes such changes provide a cellular base for explanation of decreased vision in humans with aging other than reflection errors. Effect of aging was not only qualitative, but also quantitative.

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Keywords

retina, ageing, ultrastructure, histology, immunohistochemical

About this article
Title

Aging changes in the retina of male albino rat: histological, ultrastructural and immunohistochemical study

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2018-08-24

DOI

10.5603/FM.a2018.0075

Pubmed

30155876

Keywords

retina
ageing
ultrastructure
histology
immunohistochemical

Authors

Mohamed Mohamed
Ehab Abdel Aziz El-Shaarawy
Magdy Youakim
Doaa Shuaib
Mai Ahmed

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