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Vol 78, No 2 (2019)
Original article
Submitted: 2018-06-04
Accepted: 2018-07-09
Published online: 2018-08-31
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The effect of diclofenac sodium on neural tube development in the early stage of chick embryos

T. Ertekin1, A. Bilir1, E. Aslan2, B. Koca3, O. Turamanlar1, A. Ertekin4, S. Albay5
DOI: 10.5603/FM.a2018.0080
·
Pubmed: 30178461
·
Folia Morphol 2019;78(2):307-313.
Affiliations
  1. Department of Anatomy, University of Afyon Kocatepe, School of Medicine, Afyonkarahisar, Turkey
  2. Department of Histology and Embryology, University of Afyon Kocatepe, School of Medicine, Afyonkarahisar, Turkey
  3. Department of Biochemistry, University of Afyon Kocatepe, School of Medicine, Afyonkarahisar, Turkey
  4. Emergency Medicine, Afyonkarahisar State Hospital, Afyonkarahisar, Turkey
  5. Department of Anatomy, Faculty of Medicine, Süleyman Demirel University, Isparta, Turkey

open access

Vol 78, No 2 (2019)
ORIGINAL ARTICLES
Submitted: 2018-06-04
Accepted: 2018-07-09
Published online: 2018-08-31

Abstract

Background: Neural tube defects are congenital malformations of the central nervous system. Genetic predisposition and some environmental factors play an important role in the development of neural tube defects. This study aimed to investigate the effects of diclofenac sodium on the neural tube development in a chick embryo model that corresponds to the first month of vertebral deve- lopment in mammals. 

Materials and methods: Seventy-five fertile, specific pathogen-free eggs were incubated for 28 h and were divided into five groups of 15 eggs each. Diclofenac sodium was administered via the sub-blastodermic route at this stage. Incubation was continued till the end of the 48th h. All eggs were then opened and embryos were dissected from embryonic membranes and evaluated morphologically and histopathologically. 

Results: It was determined that the use of increasing doses of diclofenac sodium led to defects of midline closure in early chicken embryos. There were statistically significant differences in neural tube positions (open or close) among the groups. In addition; crown–rump length, somite number were significantly decreased in high dose experimental groups compared with control group. 

Conclusions: This study showed that development of neurons is affected in chi- cken embryos after administration of diclofenac sodium. The exact teratogenic mechanism of diclofenac sodium is not clear; therefore it should be investigated. 

Abstract

Background: Neural tube defects are congenital malformations of the central nervous system. Genetic predisposition and some environmental factors play an important role in the development of neural tube defects. This study aimed to investigate the effects of diclofenac sodium on the neural tube development in a chick embryo model that corresponds to the first month of vertebral deve- lopment in mammals. 

Materials and methods: Seventy-five fertile, specific pathogen-free eggs were incubated for 28 h and were divided into five groups of 15 eggs each. Diclofenac sodium was administered via the sub-blastodermic route at this stage. Incubation was continued till the end of the 48th h. All eggs were then opened and embryos were dissected from embryonic membranes and evaluated morphologically and histopathologically. 

Results: It was determined that the use of increasing doses of diclofenac sodium led to defects of midline closure in early chicken embryos. There were statistically significant differences in neural tube positions (open or close) among the groups. In addition; crown–rump length, somite number were significantly decreased in high dose experimental groups compared with control group. 

Conclusions: This study showed that development of neurons is affected in chi- cken embryos after administration of diclofenac sodium. The exact teratogenic mechanism of diclofenac sodium is not clear; therefore it should be investigated. 

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Keywords

diclofenac sodium; chick embryo; neural tube defect; development

About this article
Title

The effect of diclofenac sodium on neural tube development in the early stage of chick embryos

Journal

Folia Morphologica

Issue

Vol 78, No 2 (2019)

Article type

Original article

Pages

307-313

Published online

2018-08-31

Page views

4151

Article views/downloads

2498

DOI

10.5603/FM.a2018.0080

Pubmed

30178461

Bibliographic record

Folia Morphol 2019;78(2):307-313.

Keywords

diclofenac sodium
chick embryo
neural tube defect
development

Authors

T. Ertekin
A. Bilir
E. Aslan
B. Koca
O. Turamanlar
A. Ertekin
S. Albay

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