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ORIGINAL ARTICLES
Published online: 2018-08-31
Submitted: 2018-06-04
Accepted: 2018-07-09
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The effect of diclofenac sodium on neural tube development in the early stage of chick embryos

Tolga Ertekin, Abdülkadir Bilir, Esra Aslan, Bugra Koca, Ozan Turamanlar, Ayse Ertekin, Soner Albay
DOI: 10.5603/FM.a2018.0080
·
Pubmed: 30178461

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2018-08-31
Submitted: 2018-06-04
Accepted: 2018-07-09

Abstract

Background: Neural tube defects are congenital malformations of the central nervous system. Genetic predisposition and some environmental factors play an important role in the development of neural tube defects. This study aimed to investigate the effects of Diclofenac sodium on the neural tube development in a chick embryo model that corresponds to the first month of vertebral development in mammals.

Methods: Seventy-five fertile, specific pathogen-free eggs were incubated for 28 hours and were divided into five groups of 15 eggs each. Diclofenac sodium was administered via the sub-blastodermic route at this stage. Incubation was continued till the end of the 48th hour. All eggs were then opened and embryos were dissected from embryonic membranes and evaluated morphologically and histopathologically.

Results: It was determined that the use of increasing doses of Diclofenac sodium led to defects of midline closure in early chicken embryos. There were statisticaly significant differences in neural tube positions (open or close) among the groups. In addition; crown- -rump length, somite number were significantly decreased in high dose experimental groups compared with control group.

Conclusions: This study showed that development of neurons are affected in chicken embryos after administration of diclofenac sodium. the exact teratogenic mechanism of diclofenac sodium is not clear; therefore it should be investigated.

Abstract

Background: Neural tube defects are congenital malformations of the central nervous system. Genetic predisposition and some environmental factors play an important role in the development of neural tube defects. This study aimed to investigate the effects of Diclofenac sodium on the neural tube development in a chick embryo model that corresponds to the first month of vertebral development in mammals.

Methods: Seventy-five fertile, specific pathogen-free eggs were incubated for 28 hours and were divided into five groups of 15 eggs each. Diclofenac sodium was administered via the sub-blastodermic route at this stage. Incubation was continued till the end of the 48th hour. All eggs were then opened and embryos were dissected from embryonic membranes and evaluated morphologically and histopathologically.

Results: It was determined that the use of increasing doses of Diclofenac sodium led to defects of midline closure in early chicken embryos. There were statisticaly significant differences in neural tube positions (open or close) among the groups. In addition; crown- -rump length, somite number were significantly decreased in high dose experimental groups compared with control group.

Conclusions: This study showed that development of neurons are affected in chicken embryos after administration of diclofenac sodium. the exact teratogenic mechanism of diclofenac sodium is not clear; therefore it should be investigated.

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Keywords

diclofenac sodium, chick embryo, neural tube defect, development

About this article
Title

The effect of diclofenac sodium on neural tube development in the early stage of chick embryos

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2018-08-31

DOI

10.5603/FM.a2018.0080

Pubmed

30178461

Keywords

diclofenac sodium
chick embryo
neural tube defect
development

Authors

Tolga Ertekin
Abdülkadir Bilir
Esra Aslan
Bugra Koca
Ozan Turamanlar
Ayse Ertekin
Soner Albay

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