open access

Vol 78, No 1 (2019)
ORIGINAL ARTICLES
Published online: 2018-07-17
Submitted: 2018-05-09
Accepted: 2018-06-27
Get Citation

Vitamin C attenuates the toxic effect of nutmeg on primary visual occipital cortex in rats

N. A. A. F. A. Salman, F. E.-N. A.-H. El-Safty, M. M. El-Habeby, W. B. El-Kholy, G. F. A. El-Akabawy
DOI: 10.5603/FM.a2018.0066
·
Pubmed: 30106466
·
Folia Morphol 2019;78(1):33-38.

open access

Vol 78, No 1 (2019)
ORIGINAL ARTICLES
Published online: 2018-07-17
Submitted: 2018-05-09
Accepted: 2018-06-27

Abstract

Background: Nutmeg is neurotoxic in rats and possibly neurotoxic also in hu- mans. The aim of this study is to investigate the effect of nutmeg on the primary visual occipital cortex of adult male rat and to evaluate the possible protective role of vitamin C. 

Materials and methods: Fifty Sprague-Dawley adults male rats were randomly divided into three main groups; control, nutmeg-treated (500 and 1000 mg/kg/ /day) and protected groups (nutmeg + vitamin C [500 mg/kg/day]). All rats were treated orally by gavage for 5 days per week for 6 weeks. At the end of the experiment, primary visual occipital cerebral cortex was subjected to histological, immunohistochemical and genetic analyses. 

Results: Our results revealed toxic effects of nutmeg on the primary visual occipital cerebral cortex in adult male albino rat. This was indicated by histopathological alterations, including pyknotic nuclei surrounded with vacuolations by light micro- scopic studies and degenerations of organelles by electron microscopic studies. In addition, we detected an increase in immunoreactivity for GFAP and caspase-3 by immunohistochemical assessments. Apoptotic bands appeared in genetic studies. Co-administration of vitamin C ameliorated nutmeg-induced toxic alterations on the primary visual occipital cerebral cortex. 

Conclusions: Nutmeg administration caused histopathological and genetic changes in the primary visual occipital cerebral cortex in adult male albino rats. These changes were improved by co-administration of vitamin C. 

Abstract

Background: Nutmeg is neurotoxic in rats and possibly neurotoxic also in hu- mans. The aim of this study is to investigate the effect of nutmeg on the primary visual occipital cortex of adult male rat and to evaluate the possible protective role of vitamin C. 

Materials and methods: Fifty Sprague-Dawley adults male rats were randomly divided into three main groups; control, nutmeg-treated (500 and 1000 mg/kg/ /day) and protected groups (nutmeg + vitamin C [500 mg/kg/day]). All rats were treated orally by gavage for 5 days per week for 6 weeks. At the end of the experiment, primary visual occipital cerebral cortex was subjected to histological, immunohistochemical and genetic analyses. 

Results: Our results revealed toxic effects of nutmeg on the primary visual occipital cerebral cortex in adult male albino rat. This was indicated by histopathological alterations, including pyknotic nuclei surrounded with vacuolations by light micro- scopic studies and degenerations of organelles by electron microscopic studies. In addition, we detected an increase in immunoreactivity for GFAP and caspase-3 by immunohistochemical assessments. Apoptotic bands appeared in genetic studies. Co-administration of vitamin C ameliorated nutmeg-induced toxic alterations on the primary visual occipital cerebral cortex. 

Conclusions: Nutmeg administration caused histopathological and genetic changes in the primary visual occipital cerebral cortex in adult male albino rats. These changes were improved by co-administration of vitamin C. 

Get Citation

Keywords

antioxidant; apoptosis; cerebral cortex; neurotoxin; nutmeg; vitamin C

About this article
Title

Vitamin C attenuates the toxic effect of nutmeg on primary visual occipital cortex in rats

Journal

Folia Morphologica

Issue

Vol 78, No 1 (2019)

Pages

33-38

Published online

2018-07-17

DOI

10.5603/FM.a2018.0066

Pubmed

30106466

Bibliographic record

Folia Morphol 2019;78(1):33-38.

Keywords

antioxidant
apoptosis
cerebral cortex
neurotoxin
nutmeg
vitamin C

Authors

N. A. A. F. A. Salman
F. E.-N. A.-H. El-Safty
M. M. El-Habeby
W. B. El-Kholy
G. F. A. El-Akabawy

References (17)
  1. Adjen JO. Histological effects of chronic consumption of Nutmeg on the superior colliculus of adult Wistar rats. Int J Biomed Health Sciences. 2010; 1: 6.
  2. Boada J, Cutillas B, Roig T, et al. MPP(+)-induced mitochondrial dysfunction is potentiated by dopamine. Biochem Biophys Res Commun. 2000; 268(3): 916–920.
  3. De ML, Frey RJ, Longe JL. Nutmeg In project ed. Gale Encyclopedia of Alternative Medicine. Detroit, MI: Thomson Gale. 2nd Ed. 2005; 3: 1461–1463.
  4. Dumri K, Letsiri S. Pro-oxidative activity in some thai spices. Acta Horticulturae. 2005(680): 25–29.
  5. Freireich EJ, Gehan EA, Rall DP, et al. Quantitative comparison of toxicity of anticancer agents in mouse, rat, hamster, dog, monkey, and man. Cancer Chemother Rep. 1966; 50(4): 219–244.
  6. Halliwell B. How to characterize an antioxidant: an update. Biochem Soc Symp. 1995; 61: 73–101.
  7. Jyoti A, Sethi P, Sharma D. Bacopa monniera prevents from aluminium neurotoxicity in the cerebral cortex of rat brain. J Ethnopharmacol. 2007; 111(1): 56–62.
  8. Kumar V, Abbas AK, Fausto N, et al. Pathological basis of disease.7th Ed. Cellular adaptation, cell injury and cell death. Elsevier Saunders. 2008; 1: 3.
  9. Leuba G, Kraftsik R. Changes in volume, surface estimate, three-dimensional shape and total number of neurons of the human primary visual cortex from midgestation until old age. Anat Embryol (Berl). 1994; 190(4): 4351–4366.
  10. Levine M, Rumsey SC, Daruwala R, et al. Criteria and recommendations for vitamin C intake. JAMA. 1999; 281(15): 1415–1423.
  11. Pavlovic V, Pavlovic D, Kocic G, et al. Ascorbic acid modulates monosodium glutamate induced cytotoxicity in rat thymus. 2009; 110(4): 205–209.
  12. Peng Y, Kwok KHH, Yang PH, et al. Ascorbic acid inhibits ROS production, NF-kappa B activation and prevents ethanol-induced growth retardation and microencephaly. Neuropharmacology. 2005; 48(3): 426–434.
  13. Puglia CD, Powell SR. Inhibition of cellular antioxidants: a possible mechanism of toxic cell injury. Environ Health Perspect. 1984; 57: 307–311.
  14. Rahman NAA, Fazilah A, Effarizah ME. Toxicity of Nutmeg (Myristicin) Food Technology Division, School of Industrial Technology, Universiti Sains Malaysia (USM). 2015: 5.
  15. Robert C, Repetto, Sanjay S, et al. Snippet view. https://books.google.com.gh/books (1996).
  16. Rudgley R. The Encyclopedia of Psychoactive Substances. Thomas Dunne Books/St. Martin's Griffin Rationale of hypophsis. 1998.
  17. Zhang D, Hu X, Qian Li, et al. Astrogliosis in CNS pathologies: is there a role for microglia? Mol Neurobiol. 2010; 41(2-3): 232–241.

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl