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ORIGINAL ARTICLES
Published online: 2018-08-09
Submitted: 2018-04-16
Accepted: 2018-05-23
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Combination of vitamin E and L-carnitine is superior in protection against Isoproterenol-induced cardiac affection: a histopathological evidence

Etedal Abbas Huwait
DOI: 10.5603/FM.a2018.0070
·
Pubmed: 30106462

open access

Ahead of Print
ORIGINAL ARTICLES
Published online: 2018-08-09
Submitted: 2018-04-16
Accepted: 2018-05-23

Abstract

Background: L-carnitine and Vitamin E have antioxidant properties. This study aimed to assess the effectiveness of L-carnitine, Vitamin E and the combination of them in protection against isoproterenol (ISO)-induced biochemical and histopathological changes in rat heart.

Material and methods: Fifty male Wistar rats assigned to 5 groups; control, ISO-treated group (100 mg/kg), ISO+vitamin E-treated group (100 IU/kg), ISO+L-carnitine (100 mg/kg) and ISO+vitamin E+L-carnitine treated group. At the end of the experiment, serum cardiac enzyme as well as the cardiac level Malondialdehyde (MDA), antioxidant enzymes and inflammatory cytokines IL-6, TNF-alpha were assessed. Histopathological changes in the left ventricle wall was assessed using the light and electron microscopy.

Results: Treating rats with vitamin E and L-carnitine could alleviate ISO-induced changes as it significantly reduced the serum level cardiac enzymes, MDA  and IL-6, TNF-alpha and improved the antioxidants enzymes (SOD, GSPxase and GSRase). Histopathological, they improved cardiac fibers atrophy, hemorrhages between cardiac fibers, lost striations, and disturbed sarcomere structure. The combined effect of vitamin E and L-carnitine was more superior compared to the other groups.

Conclusion: Combined administration of vitamin E, L-carnitine ameliorated the biochemical and histopathological cardiac affection induced by ISO. The effect seemed to be mediated through the antioxidant and anti-inflammatory effect of vitamin E, L-carnitine. Administration of these two element is recommended for patient at risk for myocardial infarction.

Abstract

Background: L-carnitine and Vitamin E have antioxidant properties. This study aimed to assess the effectiveness of L-carnitine, Vitamin E and the combination of them in protection against isoproterenol (ISO)-induced biochemical and histopathological changes in rat heart.

Material and methods: Fifty male Wistar rats assigned to 5 groups; control, ISO-treated group (100 mg/kg), ISO+vitamin E-treated group (100 IU/kg), ISO+L-carnitine (100 mg/kg) and ISO+vitamin E+L-carnitine treated group. At the end of the experiment, serum cardiac enzyme as well as the cardiac level Malondialdehyde (MDA), antioxidant enzymes and inflammatory cytokines IL-6, TNF-alpha were assessed. Histopathological changes in the left ventricle wall was assessed using the light and electron microscopy.

Results: Treating rats with vitamin E and L-carnitine could alleviate ISO-induced changes as it significantly reduced the serum level cardiac enzymes, MDA  and IL-6, TNF-alpha and improved the antioxidants enzymes (SOD, GSPxase and GSRase). Histopathological, they improved cardiac fibers atrophy, hemorrhages between cardiac fibers, lost striations, and disturbed sarcomere structure. The combined effect of vitamin E and L-carnitine was more superior compared to the other groups.

Conclusion: Combined administration of vitamin E, L-carnitine ameliorated the biochemical and histopathological cardiac affection induced by ISO. The effect seemed to be mediated through the antioxidant and anti-inflammatory effect of vitamin E, L-carnitine. Administration of these two element is recommended for patient at risk for myocardial infarction.

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Keywords

vitamin E, L-carnitine, Isoproterenol, heart, histology, antioxidant, anti-inflammatory

About this article
Title

Combination of vitamin E and L-carnitine is superior in protection against Isoproterenol-induced cardiac affection: a histopathological evidence

Journal

Folia Morphologica

Issue

Ahead of Print

Published online

2018-08-09

DOI

10.5603/FM.a2018.0070

Pubmed

30106462

Keywords

vitamin E
L-carnitine
Isoproterenol
heart
histology
antioxidant
anti-inflammatory

Authors

Etedal Abbas Huwait

References (35)
  1. Ahmed MM, Ibrahim ZS, Alkafafy M, et al. L-carnitine protects against testicular dysfunction caused by gamma irradiation in mice. Acta Histochem. 2014; 116(6): 1046–1055.
  2. Alshiek JA, Dayan L, Asleh R, et al. Anti-oxidative treatment with vitamin E improves peripheral vascular function in patients with diabetes mellitus and Haptoglobin 2-2 genotype: A double-blinded cross-over study. Diabetes Res Clin Pract. 2017; 131: 200–207.
  3. Bancroft JD, Gamble M. Theory and practice of histological techniques. 6th ed. Churchill Livingstone, Elsevier, China. 2008.
  4. Boaz M, Smetana S, Weinstein T, et al. Secondary prevention with antioxidants of cardiovascular disease in endstage renal disease (SPACE): randomised placebo-controlled trial. Lancet. 2000; 356(9237): 1213–1218.
  5. De Bono DP, Boon NA. Diseases of the cardiovascular system. In: Edwards CRW, Bouchier IAS, eds. Davidson’s Principles of Practice and Medicine. Hong Kong: Churchill Livingstone. 1992: 249–340.
  6. Deore AB, Vinayak D. Sapakal and Naikwade NS: Antioxidant and hepatoprotective activity of Garcinia indica Linn fruit rind. J Comprehensive Pharmacy. 6.08.
  7. Dhivya V, Priya LB, Chirayil HT, et al. Piperine modulates isoproterenol induced myocardial ischemia through antioxidant and anti-dyslipidemic effect in male Wistar rats. Biomed Pharmacother. 2017; 87: 705–713.
  8. Gayathri K, Jayachandran KS, Vasanthi HR, et al. Cardioprotective effect of lemon grass as evidenced by biochemical and histopathological changes in experimentally induced cardiotoxicity. Hum Exp Toxicol. 2011; 30(8): 1073–1082.
  9. Goyal S, Siddiqui MK, Siddiqui KM, et al. Cardioprotective effect of 'Khamira Abresham Hakim Arshad Wala' a unani formulation in isoproterenol-induced myocardial necrosis in rats. Exp Toxicol Pathol. 2010; 62(1): 61–74.
  10. Goyal SN, Arora S, Sharma AK, et al. Preventive effect of crocin of Crocus sativus on hemodynamic, biochemical, histopathological and ultrastuctural alterations in isoproterenol-induced cardiotoxicity in rats. Phytomedicine. 2010; 17(3-4): 227–232.
  11. Goyal SN, Sharma C, Mahajan UB, et al. Protective effects of cardamom in isoproterenol-induced myocardial infarction in rats. Int J Mol Sci. 2015; 16(11): 27457–27469.
  12. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem. 1974; 249(22): 7130–7139.
  13. Hu XX, Fu Li, Li Y, et al. The cardioprotective effect of vitamin e (alpha-tocopherol) is strongly related to age and gender in mice. PLoS One. 2015; 10(9): e0137405.
  14. Lango R, Smolenski RT, Narkiewicz M, et al. Influence of L-carnitine and its derivatives on myocardial metabolism and function in ischemic heart disease and during cardiopulmonary bypass. Cardiovasc Res. 2001; 51(1): 21–29.
  15. Lee BJ, Lin JS, Lin YC, et al. Effects of L-carnitine supplementation on lipid profiles in patients with coronary artery disease. Lipids Health Dis. 2016; 15: 107.
  16. Lee IM, Cook N, Gaziano J, et al. Vitamin E in the primary prevention of cardiovascular disease and cancer. The Women's Health Study: a randomized controlled trial. JAMA. 2005; 294(1): 56–65.
  17. Li Li, Zhu Di, Sun X. [Cellular uptake of TPS-L-carnitine synthesised as transporter-based renal targeting prodrug]. Sichuan Da Xue Xue Bao Yi Xue Ban. 2012; 43(6): 936–940.
  18. Liepinsh E, Makrecka-Kuka M, Kuka J, et al. Inhibition of L-carnitine biosynthesis and transport by methyl-γ-butyrobetaine decreases fatty acid oxidation and protects against myocardial infarction. Br J Pharmacol. 2015; 172(5): 1319–1332.
  19. Panteghini M. Role and importance of biochemical markers in clinical cardiology. Eur Heart J. 2004; 25(14): 1187–1196.
  20. Milman U, Blum S, Shapira C, et al. Vitamin E supplementation reduces cardiovascular events in a subgroup of middle-aged individuals with both type 2 diabetes mellitus and the haptoglobin 2-2 genotype: a prospective double-blinded clinical trial. Arterioscler Thromb Vasc Biol. 2008; 28(2): 341–347.
  21. Moran MS. Levels of glutathione, glutathione reductase and glutathione-S-transferase activity in rat lung and liver. Biochim et Biophys. 1979; 582: 67–78.
  22. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70(1): 158–169.
  23. Panda S, Kar A. Combined effects of vincristine and quercetin in reducing isoproterenol-induced cardiac necrosis in rats. Cardiovasc Toxicol. 2015; 15(4): 291–299.
  24. Panda V, Kamble S, Desai Y, et al. Antioxidant and cardioprotective effects of Garcinia indica (kokoberry), an Indian super fruit in isoproterenol induced myocardial necrosis in rats. J Berry Res. 2014; 4(3): 159–174.
  25. Salama A, Kasem S, Tousson E, et al. L-carnitine and vitamin E alleviate reproductive toxicity caused by triton WR 1339 in male albino rats. Toxicol Ind Health. 2015; 31(5): 467–474.
  26. Schönekess B, Lopaschuk G. The effects of carnitine on myocardial carbohydrate metabolism. The Carnitine System. A New Therapeutical Approach to Cardiovascular Diseases, edited by De Jong JW, Ferrari R, Dordrecht, Kluwer Academic Publishers. 1995: 39–52.
  27. Shang R, Sun Z, Li H. Effective dosing of L-carnitine in the secondary prevention of cardiovascular disease: a systematic review and meta-analysis. BMC Cardiovasc Disord. 2014; 14: 88.
  28. Shukla SK, Sharma SB, Singh UR, et al. Eugenia jambolana pretreatment prevents isoproterenol-induced myocardial damage in rats: evidence from biochemical, molecular, and histopathological studies. J Med Food. 2014; 17(2): 244–253.
  29. Tappia PS, Hata T, Hozaima L, et al. Role of oxidative stress in catecholamine-induced changes in cardiac sarcolemmal Ca2+ transport. Arch Biochem Biophys. 2001; 387(1): 85–92.
  30. Thygesen K, Mair J, Katus H, et al. Acute Cardiac Care. Recomendations for the use of cardiac troponin measurement in acute cardiac care. Eur Heart J. 2010; 31(18): 2197–2204.
  31. Tousson E, Hafez E, Zaki S, et al. The cardioprotective effects of L-carnitine on rat cardiac injury, apoptosis, and oxidative stress caused by amethopterin. Environ Sci Pollut Res Int. 2016; 23(20): 20600–20608.
  32. Whellan D. Heart failure disease management. Implementation and outcomes. Cardiol Rev. 2005; 13(5): 231–239.
  33. Wong GK, Pehora C, Crawford MW. L-carnitine reduces susceptibility to bupivacaine-induced cardiotoxicity: an experimental study in rats. Can J Anaesth. 2017; 64(3): 270–279.
  34. Yüncü M, Bükücü N, Bayat N, et al. The effect of vitamin E and L-carnitine against methotrexate-induced injury in rat testis. Turk J Med Sci. 2015; 45(3): 517–525.
  35. Zambrano S, Blanca AJ, Ruiz-Armenta MV, et al. L-carnitine protects against arterial hypertension-related cardiac fibrosis through modulation of PPAR-γ expression. Biochem Pharmacol. 2013; 85(7): 937–944.

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