open access

Vol 77, No 3 (2018)
ORIGINAL ARTICLES
Published online: 2018-01-11
Submitted: 2017-12-18
Accepted: 2017-12-27
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Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

B. Deveci, B. Ayna, İ. H. Tacir, E. Deveci, M. C. Tuncer, A. Pala
DOI: 10.5603/FM.a2018.0004
·
Pubmed: 29345720
·
Folia Morphol 2018;77(3):471-477.

open access

Vol 77, No 3 (2018)
ORIGINAL ARTICLES
Published online: 2018-01-11
Submitted: 2017-12-18
Accepted: 2017-12-27

Abstract

Background: Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of matrix metalloproteinases 2 (MMPs2) and vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane.
Materials and methods: In this experimental study, rats were divided into nicotine and control groups. While the rats in the nicotine group (n = 6) were administered 2 mg/kg nicotine sulphate for 28 days, the animals in the control group (n = 6) were only administered 1.5 mL physiologic saline solution subcutaneously for 28 days.
Results: Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining.
Conclusions: Nicotine reduces MMP production, disrupts collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevents tooth to anchor in dental alveoli by disrupting epithelial structure.

Abstract

Background: Nicotine is associated with increased incidence of periodontal disease and poor response to therapy. This article aimed at identifying the expression of matrix metalloproteinases 2 (MMPs2) and vascular endothelial growth factor (VEGF) proteins on extracellular matrix, fibrous distribution and angiogenetic development in periodontitis caused by nicotine effects on periodontal membrane.
Materials and methods: In this experimental study, rats were divided into nicotine and control groups. While the rats in the nicotine group (n = 6) were administered 2 mg/kg nicotine sulphate for 28 days, the animals in the control group (n = 6) were only administered 1.5 mL physiologic saline solution subcutaneously for 28 days.
Results: Histological sections were prepared and immunohistochemically stained for MMP2 and VEGF. The sections stained with Trichrome-Masson were observed under light microscope. VEGF and MMP2 immunoreactivity of periodontal gingiva and dentin was assessed by immunohistochemical staining.
Conclusions: Nicotine reduces MMP production, disrupts collagen synthesis and causes periodontitis. We observed that nicotine increases periodontitis by disrupting periodontal membrane and prevents tooth to anchor in dental alveoli by disrupting epithelial structure.

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Keywords

MMP2; VEGF; periodontal membrane; rat; immunohistochemistry; nicotine

About this article
Title

Effects of nicotine administration in rats on MMP2 and VEGF levels in periodontal membrane

Journal

Folia Morphologica

Issue

Vol 77, No 3 (2018)

Pages

471-477

Published online

2018-01-11

DOI

10.5603/FM.a2018.0004

Pubmed

29345720

Bibliographic record

Folia Morphol 2018;77(3):471-477.

Keywords

MMP2
VEGF
periodontal membrane
rat
immunohistochemistry
nicotine

Authors

B. Deveci
B. Ayna
İ. H. Tacir
E. Deveci
M. C. Tuncer
A. Pala

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