Multimedialna platforma wiedzy medycznej Internetowa Księgarnia Medyczna Kalendarium Konferencji
Folia Morphologica
MENU
Shortcuts Submit an article Author Guidelines (new) Ahead Of Print Reviewers 2023

open access

Vol 77, No 3 (2018)
Original article
Submitted: 2017-09-20
Accepted: 2017-10-28
Published online: 2017-11-06
View PDF Download PDF file
Get Citation

Fractal and stereological analyses of insulin-induced rat exocrine pancreas remodelling

M. Pajevic1, M. Aleksic2, I. Golic2, M. Markelic2, V. Otasevic3, A. Jankovic3, A. Stancic3, B. Korac3, A. Korac2
DOI: 10.5603/FM.a2017.0106
·
Pubmed: 29131278
·
Folia Morphol 2018;77(3):478-484.
Affiliations
  1. Department of Histopathology, Clinical Centre of Serbia, Belgrade, Serbia
  2. Center for Electron Microscopy, University of Belgrade, Faculty of Biology, Belgrade, Serbia
  3. Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, Belgrade, Serbia

open access

Vol 77, No 3 (2018)
ORIGINAL ARTICLES
Submitted: 2017-09-20
Accepted: 2017-10-28
Published online: 2017-11-06

Abstract

Background: The effect of insulin on the endocrine pancreas has been the subject of extensive study, but quantitative morphometric investigations of the exocrine pancreas are scarce. This study was therefore undertaken to investigate the effect of acute and chronic insulin administration (two doses, 0.4 IU and 4 IU) on the morphology of rat pancreas acini. Materials and methods: Semi-fine sections stained with methylene blue and basic fuchsine or haematoxylin and eosin-stained 5-micrometer thick paraffin sections were used for fractal and stereological analysis of exocrine acini. Acute insulin treatment, independent of applied doses increased fractal dimension in line with decreased lacunarity of pancreas acini. Chronic low dose insulin decreased fractal dimension and increased lacunarity of pancreas acini, but a high dose had the opposite effect. The volume densities (Vv) of cytoplasm, granules and nucleus are affected differently: acute low dose and high chronic dose significantly decreased granules Vv, and in line increased cytoplasmic Vv, whereas other examined structures showed slight changes without statistical significance. Results: The results obtained from this investigation indicate that insulin treatment induced structural remodelling of the exocrine pancreas suggesting a substantial role of insulin in its functioning. Conclusions: Additionally, we showed that fine architectural changes in acini could be detected by fractal analysis, suggesting this method as an alternative or addition to routine stereology.

Abstract

Background: The effect of insulin on the endocrine pancreas has been the subject of extensive study, but quantitative morphometric investigations of the exocrine pancreas are scarce. This study was therefore undertaken to investigate the effect of acute and chronic insulin administration (two doses, 0.4 IU and 4 IU) on the morphology of rat pancreas acini. Materials and methods: Semi-fine sections stained with methylene blue and basic fuchsine or haematoxylin and eosin-stained 5-micrometer thick paraffin sections were used for fractal and stereological analysis of exocrine acini. Acute insulin treatment, independent of applied doses increased fractal dimension in line with decreased lacunarity of pancreas acini. Chronic low dose insulin decreased fractal dimension and increased lacunarity of pancreas acini, but a high dose had the opposite effect. The volume densities (Vv) of cytoplasm, granules and nucleus are affected differently: acute low dose and high chronic dose significantly decreased granules Vv, and in line increased cytoplasmic Vv, whereas other examined structures showed slight changes without statistical significance. Results: The results obtained from this investigation indicate that insulin treatment induced structural remodelling of the exocrine pancreas suggesting a substantial role of insulin in its functioning. Conclusions: Additionally, we showed that fine architectural changes in acini could be detected by fractal analysis, suggesting this method as an alternative or addition to routine stereology.

Full Text:

View PDF Download PDF file
Get Citation

Keywords

insulin; pancreas acini; fractal analysis; stereology

About this article
Title

Fractal and stereological analyses of insulin-induced rat exocrine pancreas remodelling

Journal

Folia Morphologica

Issue

Vol 77, No 3 (2018)

Article type

Original article

Pages

478-484

Published online

2017-11-06

Page views

2169

Article views/downloads

1139

DOI

10.5603/FM.a2017.0106

Pubmed

29131278

Bibliographic record

Folia Morphol 2018;77(3):478-484.

Keywords

insulin
pancreas acini
fractal analysis
stereology

Authors

M. Pajevic
M. Aleksic
I. Golic
M. Markelic
V. Otasevic
A. Jankovic
A. Stancic
B. Korac
A. Korac

References (39)
  1. Al-Mrabeh A, Hollingsworth KG, Steven S, et al. Morphology of the pancreas in type 2 diabetes: effect of weight loss with or without normalisation of insulin secretory capacity. Diabetologia. 2016; 59(8): 1753–1759.
    CrossRefPubMed
  2. Ashcroft FM, Rorsman P. Diabetes mellitus and the β cell: the last ten years. Cell. 2012; 148(6): 1160–1171.
    CrossRefPubMed
  3. Aughsteen AA, Mohammed FI. Insulin enhances amylase and lipase activity in the pancreas of streptozotocin-diabetic rats. An in vivo study. Saudi Med J. 2002; 23(7): 838–844.
    PubMed
  4. Bani D, Magnani L, Sacchi TB, et al. The exocrine pancreas in patients with hyperinsulinemic hypoglycemia. A morphometrical and ultrastructural study. Int J Pancreatol. 1989; 5(3): 239–248.
    PubMed
  5. Barreto SG, Carati CJ, Toouli J, et al. The islet-acinar axis of the pancreas: more than just insulin. Am J Physiol Gastrointest Liver Physiol. 2010; 299(1): G10–G22.
    CrossRefPubMed
  6. Bizzarri M, Giuliani A, Cucina A, et al. Fractal analysis in a systems biology approach to cancer. Semin Cancer Biol. 2011; 21(3): 175–182.
    CrossRefPubMed
  7. Blanco-Molina A, González-Reyes JA, Torre-Cisneros J, et al. Effects of hypothyroidism on the ultrastructure of rat pancreatic acinar cells: a stereological analysis. Histol Histopathol. 1991; 6(1): 37–42.
    PubMed
  8. Campbell-Thompson M, Rodriguez-Calvo T, Battaglia M. Abnormalities of the exocrine pancreas in type 1 diabetes. Curr Diab Rep. 2015; 15(10): 79.
    CrossRefPubMed
  9. Cano DA, Hebrok M, Zenker M. Pancreatic development and disease. Gastroenterology. 2007; 132(2): 745–762.
    CrossRefPubMed
  10. Cordain L, Eades MR, Eades MD. Hyperinsulinemic diseases of civilization: more than just Syndrome X. Comp Biochem Physiol A Mol Integr Physiol. 2003; 136(1): 95–112.
    PubMed
  11. Corkey BE. Banting lecture 2011: hyperinsulinemia: cause or consequence? Diabetes. 2012; 61(1): 4–13.
    CrossRefPubMed
  12. Ferro DP, Falconi MA, Adam RL, et al. Fractal characteristics of May-Grünwald-Giemsa stained chromatin are independent prognostic factors for survival in multiple myeloma. PLoS One. 2011; 6(6): e20706.
    CrossRefPubMed
  13. Gheonea DI, Streba CT, Vere CC, et al. Diagnosis system for hepatocellular carcinoma based on fractal dimension of morphometric elements integrated in an artificial neural network. Biomed Res Int. 2014; 2014: 239706.
    CrossRefPubMed
  14. Gonzalez E, Flier E, Molle D, et al. Hyperinsulinemia leads to uncoupled insulin regulation of the GLUT4 glucose transporter and the FoxO1 transcription factor. Proc Natl Acad Sci U S A. 2011; 108(25): 10162–10167.
    CrossRefPubMed
  15. Gudea AI, Stefan AC. Histomorphometric, fractal and lacunarity comparative analysis of sheep (Ovis aries), goat (Capra hircus) and roe deer (Capreolus capreolus) compact bone samples. Folia Morphol. 2013; 72(3): 239–248.
    PubMed
  16. Hamamoto N, Ashizawa N, Niigaki M, et al. Morphological changes in the rat exocrine pancreas after pancreatic duct ligation. Histol Histopathol. 2002; 17(4): 1033–1041.
    CrossRefPubMed
  17. Hayden MR, Patel K, Habibi J, et al. Attenuation of endocrine-exocrine pancreatic communication in type 2 diabetes: pancreatic extracellular matrix ultrastructural abnormalities. J Cardiometab Syndr. 2008; 3(4): 234–243.
    CrossRefPubMed
  18. Hotar V, Salac P. Surface evaluation by estimation of fractal dimension and statistical tools. Sci World J. 2014; 2014: 435935.
    CrossRefPubMed
  19. Jo J, Hörnblad A, Kilimnik G, et al. The fractal spatial distribution of pancreatic islets in three dimensions: a self-avoiding growth model. Phys Biol. 2013; 10(3): 036009.
    CrossRefPubMed
  20. Johnson JD, Alejandro EU. Control of pancreatic beta-cell fate by insulin signaling: The sweet spot hypothesis. Cell Cycle. 2008; 7(10): 1343–1347.
    CrossRefPubMed
  21. Mehran AE, Templeman NM, Brigidi GS, et al. Hyperinsulinemia drives diet-induced obesity independently of brain insulin production. Cell Metab. 2012; 16(6): 723–737.
    CrossRefPubMed
  22. Metze K. Fractal dimension of chromatin: potential molecular diagnostic applications for cancer prognosis. Expert Rev Mol Diagn. 2013; 13(7): 719–735.
    CrossRefPubMed
  23. Motta PM, Macchiarelli G, Nottola SA, et al. Histology of the exocrine pancreas. Microsc Res Tech. 1997; 37(5-6): 384–398.
    CrossRefPubMed
  24. Pantic I, Nesic D, Stevanovic D, et al. Effects of ghrelin on the structural complexity of exocrine pancreas tissue architecture. Microsc Microanal. 2013; 19(3): 553–558.
    CrossRefPubMed
  25. Pap A. Effects of insulin and glucose metabolism on pancreatic exocrine function. Int J Diabetes Metab. 2004; 12: 30–34.
  26. Piciucchi M, Capurso G, Archibugi L, et al. Exocrine pancreatic insufficiency in diabetic patients: prevalence, mechanisms, and treatment. Int J Endocrinol. 2015; 2015: 595649.
    CrossRefPubMed
  27. Rhodes CJ, White MF. Molecular insights into insulin action and secretion. Eur J Clin Invest. 2002; 32 Suppl 3: 3–13.
    PubMed
  28. Saito A, Williams JA, Kanno T. Potentiation of cholecystokinin-induced exocrine secretion by both exogenous and endogenous insulin in isolated and perfused rat pancreata. J Clin Invest. 1980; 65(4): 777–782.
    CrossRefPubMed
  29. Samuel VT, Shulman GI. Mechanisms for insulin resistance: common threads and missing links. Cell. 2012; 148(5): 852–871.
    CrossRefPubMed
  30. Shanik MH, Xu Y, Skrha J, et al. Insulin resistance and hyperinsulinemia: is hyperinsulinemia the cart or the horse? Diabetes Care. 2008; 31 Suppl 2: S262–S268.
    CrossRefPubMed
  31. Sirri R, Bianco C, De Vico G, et al. Proliferation, apoptosis, and fractal dimension analysis for the quantification of intestinal trophism in sole (Solea solea) fed mussel meal diets. BMC Vet Res. 2014; 10: 148.
    CrossRefPubMed
  32. Slavin BG, Zarow C, Warden CH, et al. Histological, immunocytochemical, and morphometrical analyses of pancreatic islets in the BSB mouse model of obesity. Anat Rec (Hoboken). 2010; 293(1): 108–116.
    CrossRefPubMed
  33. Taga R, Faverão MR, Cestari TM, et al. Morphometric dimensions of Syrian golden hamster pancreas of both sexes. Okajimas Folia Anat Jpn. 1999; 76(1): 41–46.
    PubMed
  34. Weibel ER, Kistler GS, Scherle WF. Practical stereological methods for morphometric cytology. J Cell Biol. 1966; 30(1): 23–38.
    PubMed
  35. West BJ. Fractal physiology and the fractional calculus: a perspective. Front Physiol. 2010; 1: 12.
    CrossRefPubMed
  36. Wilcox G. Insulin and insulin resistance. Clin Biochem Rev. 2005; 26(2): 19–39.
  37. Williams JA, Goldfine ID. The insulin-pancreatic acinar axis. Diabetes. 1985; 34(10): 980–986.
    PubMed
  38. Williams JA. Regulation of acinar cell function in the pancreas. Curr Opin Gastroenterol. 2010; 26(5): 478–483.
    CrossRefPubMed
  39. Yang X, Mei S, Gu H, et al. Exposure to excess insulin (glargine) induces type 2 diabetes mellitus in mice fed on a chow diet. J Endocrinol. 2014; 221(3): 469–480.
    CrossRefPubMed

Regulations

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By VM Media Group sp. z o.o., Grupa Via Medica, Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.: +48 58 320 94 94, faks: +48 58 320 94 60, e-mail: viamedica@viamedica.pl