open access

Vol 77, No 2 (2018)
ORIGINAL ARTICLES
Published online: 2017-10-17
Submitted: 2017-07-24
Accepted: 2017-10-05
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Comparison of type I collagens and MMP-2 proteins in temporomandibular joint of young and old mice

M. Demir, M.K. Tumer, M. Çiçek, M. Uysal, A. Yoldas, A. Doganer
DOI: 10.5603/FM.a2017.0096
·
Pubmed: 29064547
·
Folia Morphol 2018;77(2):329-334.

open access

Vol 77, No 2 (2018)
ORIGINAL ARTICLES
Published online: 2017-10-17
Submitted: 2017-07-24
Accepted: 2017-10-05

Abstract

Background: The effects of ageing on the histopathological changes of tem­poromandibular joint (TMJ) and the existence and age related alterations of immunochemical expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) proteins was aimed to be displayed.

Materials and methods: In this study, 14 Balb/C type white mice (50– –80 g) were included. Groups were organised as group 1 — 2-month-old young animals (n = 7) and group 2 — 18-month-old old animals (n = 7). Of the paraffin embedded tissues 4–5 μm thick sections were taken and immunohisto­chemical stainings of haematoxylin-eosin, type-1 collagen and MMP-2 were performed.

Results: Collagen bundles showed sagittal and oblique localisations in the young mice, which were comprised of compact collagen bundle layers positioned alterna­tely. While collagen bundle fragmentation was observed in the disks of old mice, some disk regions showed ruptures. In the old mice a decrease in blood vessels, structural impairments and dilatation in arterioles and venules were detected. In the TMJ tissues of the young mice type I collagen and MMP-2 expressions were increased, while they were decreased in old mice. In the MMP-2 H-score evaluation young mice showed significant increase compared to the old mice.

Conclusions: Occurrence of degenerations in the collagen structure of TMJ and decimation in the matrix metalloproteases were observed with age. (Folia Morphol 2018; 77, 2: 329–334)

Abstract

Background: The effects of ageing on the histopathological changes of tem­poromandibular joint (TMJ) and the existence and age related alterations of immunochemical expressions of type I collagen and matrix metalloproteinase-2 (MMP-2) proteins was aimed to be displayed.

Materials and methods: In this study, 14 Balb/C type white mice (50– –80 g) were included. Groups were organised as group 1 — 2-month-old young animals (n = 7) and group 2 — 18-month-old old animals (n = 7). Of the paraffin embedded tissues 4–5 μm thick sections were taken and immunohisto­chemical stainings of haematoxylin-eosin, type-1 collagen and MMP-2 were performed.

Results: Collagen bundles showed sagittal and oblique localisations in the young mice, which were comprised of compact collagen bundle layers positioned alterna­tely. While collagen bundle fragmentation was observed in the disks of old mice, some disk regions showed ruptures. In the old mice a decrease in blood vessels, structural impairments and dilatation in arterioles and venules were detected. In the TMJ tissues of the young mice type I collagen and MMP-2 expressions were increased, while they were decreased in old mice. In the MMP-2 H-score evaluation young mice showed significant increase compared to the old mice.

Conclusions: Occurrence of degenerations in the collagen structure of TMJ and decimation in the matrix metalloproteases were observed with age. (Folia Morphol 2018; 77, 2: 329–334)

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Keywords

temporomandibular joint, type I collagen, MMP-2, matrix metalloproteinase-2, immunreactivity, ageing

About this article
Title

Comparison of type I collagens and MMP-2 proteins in temporomandibular joint of young and old mice

Journal

Folia Morphologica

Issue

Vol 77, No 2 (2018)

Pages

329-334

Published online

2017-10-17

DOI

10.5603/FM.a2017.0096

Pubmed

29064547

Bibliographic record

Folia Morphol 2018;77(2):329-334.

Keywords

temporomandibular joint
type I collagen
MMP-2
matrix metalloproteinase-2
immunreactivity
ageing

Authors

M. Demir
M.K. Tumer
M. Çiçek
M. Uysal
A. Yoldas
A. Doganer

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