open access

Vol 76, No 3 (2017)
ORIGINAL ARTICLES
Published online: 2017-02-14
Submitted: 2016-09-05
Accepted: 2016-11-17
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The expression of inhibitor of nuclear factor kappa-B kinase epsilon (IKKe) in human aortic aneurysm

L. Zhang, L. Wang, W. Chen, Y. Xu, L. Wang, R. Iskandar, Y. Wang, X. Chen
DOI: 10.5603/FM.a2017.0016
·
Pubmed: 28198528
·
Folia Morphol 2017;76(3):372-378.

open access

Vol 76, No 3 (2017)
ORIGINAL ARTICLES
Published online: 2017-02-14
Submitted: 2016-09-05
Accepted: 2016-11-17

Abstract

Background: Aortic aneurysm (AA) is one of the most common causes of sudden death among elderly people. Although AA can be detected by non-invasive imaging techniques, there are no pharmacological treatments currently available to prevent progression at any stage of the disease. In this study we will explore the expression of inhibitor of nuclear factor kappa-B kinase epsilon (IKKe) in AA and its potential underlying molecular mechanism in AA.

Materials and methods: Human aortic tissue was taken from 14 patients who underwent surgical repair of AA for the AA group and another 11 patients with normal aorta who underwent aortic valve replacement surgery for the control group. After excision, we used haematoxylin-eosin staining, Masson staining, immunohistochemistry analysis and Western blot analysis to observe the expres­sion, location and morphological changes of the IKKe, P50 and the extracellular matrix within the AA.

Results: In the AA group, haematoxylin-eosin staining revealed a loss of medial integrity and inflammatory cell infiltration. Masson staining confirmed the degradation of the extracellular matrix in the AA group. Immunohistochemistry analysis showed increased infiltration of inflammatory cells and up-regulation of proinflammatory cytokines in the AA group when compared to the control group. Based on immunohistochemistry and Western blot analysis, there was clearly over-expression of IKKe, P50 and MMP2 in AA group, mainly in the intrinsic aortic cells of the media.

Conclusions: The over-expression of IKKe may play an important role in the ori­gination and progression of AA and might be a vital target for their treatment.

Abstract

Background: Aortic aneurysm (AA) is one of the most common causes of sudden death among elderly people. Although AA can be detected by non-invasive imaging techniques, there are no pharmacological treatments currently available to prevent progression at any stage of the disease. In this study we will explore the expression of inhibitor of nuclear factor kappa-B kinase epsilon (IKKe) in AA and its potential underlying molecular mechanism in AA.

Materials and methods: Human aortic tissue was taken from 14 patients who underwent surgical repair of AA for the AA group and another 11 patients with normal aorta who underwent aortic valve replacement surgery for the control group. After excision, we used haematoxylin-eosin staining, Masson staining, immunohistochemistry analysis and Western blot analysis to observe the expres­sion, location and morphological changes of the IKKe, P50 and the extracellular matrix within the AA.

Results: In the AA group, haematoxylin-eosin staining revealed a loss of medial integrity and inflammatory cell infiltration. Masson staining confirmed the degradation of the extracellular matrix in the AA group. Immunohistochemistry analysis showed increased infiltration of inflammatory cells and up-regulation of proinflammatory cytokines in the AA group when compared to the control group. Based on immunohistochemistry and Western blot analysis, there was clearly over-expression of IKKe, P50 and MMP2 in AA group, mainly in the intrinsic aortic cells of the media.

Conclusions: The over-expression of IKKe may play an important role in the ori­gination and progression of AA and might be a vital target for their treatment.

Get Citation

Keywords

IKKe, P50, inflammation, aortic aneurysm, metalloproteinase

About this article
Title

The expression of inhibitor of nuclear factor kappa-B kinase epsilon (IKKe) in human aortic aneurysm

Journal

Folia Morphologica

Issue

Vol 76, No 3 (2017)

Pages

372-378

Published online

2017-02-14

DOI

10.5603/FM.a2017.0016

Pubmed

28198528

Bibliographic record

Folia Morphol 2017;76(3):372-378.

Keywords

IKKe
P50
inflammation
aortic aneurysm
metalloproteinase

Authors

L. Zhang
L. Wang
W. Chen
Y. Xu
L. Wang
R. Iskandar
Y. Wang
X. Chen

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