open access

Vol 76, No 2 (2017)
ORIGINAL ARTICLES
Published online: 2016-10-10
Submitted: 2016-02-12
Accepted: 2016-08-23
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Quercetin attenuates, indomethacin-induced acute gastric ulcer in rats

A. G. R. Alkushi, N. A. M. Elsawy
DOI: 10.5603/FM.a2016.0067
·
Pubmed: 27813628
·
Folia Morphol 2017;76(2):252-261.

open access

Vol 76, No 2 (2017)
ORIGINAL ARTICLES
Published online: 2016-10-10
Submitted: 2016-02-12
Accepted: 2016-08-23

Abstract

Background: Peptic ulcer diseases are common and are induced by many factors, including stress, smoking, and ingestion of non-steroidal anti-inflammatory drugs. Quercetin is considered to be an anti-oxidant with healing effects on many experimental toxic injuries. The present study aimed to explore the possible effect of quercetin on acute gastric ulcer induced by indomethacin in rats.

Materials and methods: Three groups received indomethacin (30 mg/kg body weight) orally by orogastric gavage on two consecutive days. The rats received famotidine (50 mg/kg body weight), quercetin (50 mg/kg body weight), or vehicle alone for 15 consecutive days by oral gavage. The control group received no indomethacin but received vehicle for 15 days by oral gavage. The ulcer index, volume, and pH of gastric juice were measured, and the stomachs were examined by routine light microscopy.

Results: Compared with the control group, the indomethacin-treated rats showed a marked damage of the gastric mucosal surface and a high ulcer index. In the famotidine- and quercetin-treated groups, significantly increased antioxidant enzyme activities were observed. The congestion, erosions, and necrosis were reduced with mild inflammatory cell infiltration while no major damage of endothelial cells was observed in the treated rats.

Conclusions: The findings of the study show that quercetin had antioxidant effect and can protect gastric mucosa against indomethacin-induced gastric ulceration than famotidine.  

Abstract

Background: Peptic ulcer diseases are common and are induced by many factors, including stress, smoking, and ingestion of non-steroidal anti-inflammatory drugs. Quercetin is considered to be an anti-oxidant with healing effects on many experimental toxic injuries. The present study aimed to explore the possible effect of quercetin on acute gastric ulcer induced by indomethacin in rats.

Materials and methods: Three groups received indomethacin (30 mg/kg body weight) orally by orogastric gavage on two consecutive days. The rats received famotidine (50 mg/kg body weight), quercetin (50 mg/kg body weight), or vehicle alone for 15 consecutive days by oral gavage. The control group received no indomethacin but received vehicle for 15 days by oral gavage. The ulcer index, volume, and pH of gastric juice were measured, and the stomachs were examined by routine light microscopy.

Results: Compared with the control group, the indomethacin-treated rats showed a marked damage of the gastric mucosal surface and a high ulcer index. In the famotidine- and quercetin-treated groups, significantly increased antioxidant enzyme activities were observed. The congestion, erosions, and necrosis were reduced with mild inflammatory cell infiltration while no major damage of endothelial cells was observed in the treated rats.

Conclusions: The findings of the study show that quercetin had antioxidant effect and can protect gastric mucosa against indomethacin-induced gastric ulceration than famotidine.  

Get Citation

Keywords

flavonoids, ulceration, nonsteroidal anti-inflammatory drugs, pH value, gastric juice

About this article
Title

Quercetin attenuates, indomethacin-induced acute gastric ulcer in rats

Journal

Folia Morphologica

Issue

Vol 76, No 2 (2017)

Pages

252-261

Published online

2016-10-10

DOI

10.5603/FM.a2016.0067

Pubmed

27813628

Bibliographic record

Folia Morphol 2017;76(2):252-261.

Keywords

flavonoids
ulceration
nonsteroidal anti-inflammatory drugs
pH value
gastric juice

Authors

A. G. R. Alkushi
N. A. M. Elsawy

References (74)
  1. Abrahamse S, Kloots W, Amelsvoort Jv. Absorption, distribution, and secretion of epicatechin and quercetin in the rat. Nutr Res. 2005; 25(3): 305–317.
  2. Aguirre L, Arias N, Macarulla MT, et al. Beneficial Effects of Quercetin on Obesity and Diabetes. The Open Nutraceuticals J. 2011; 4: 189–198.
  3. Alqasoumi S, Al-Sohaibani M, Al-Howiriny T, et al. Rocket. World J Gastroenterol. 2009; 15(16): 1958–1965.
  4. Amić D, Davidović-Amić D, Beslo D, et al. SAR and QSAR of the antioxidant activity of flavonoids. Curr Med Chem. 2007; 14(7): 827–845.
  5. Asar M, Kayişli UA, Izgüt-Uysal VN, et al. Cadmium-induced changes in epithelial cells of the rat stomach. Biol Trace Elem Res. 2000; 77(1): 65–81.
  6. Asefa Z, G/eyesus A. Perforated peptic ulcer disease in Zewditu Hospital. Ethiop Med J. 2012; 50(2): 145–151.
  7. Bancroft JD, Gamble M. Theory and practice of histological techniques. Churchill Livingstone, London 2007.
  8. Boots AW, Haenen GR, Bast A. Health effects of quercetin: from antioxidant to nutraceutical. Eur J Pharmacol. 2008; 585(2-3): 325–337.
  9. Brett A, Ghica ME. Electrochemical Oxidation of Quercetin. Electroanalysis. 2003; 15(22): 1745–1750.
  10. Chapman DG, Castillo R, Campbell JA. Evaluation of protein in foods: 1. A method for the determination of protein efficiency ratios. Can J Biochem Phys. 1959; 37(5): 679–686.
  11. Chason RD, Reisch JS, Rockey DC. More favorable outcomes with peptic ulcer bleeding due to Helicobacter pylori. Am J Med. 2013; 126(9): 811–818.e1.
  12. Chatterjee A, Chatterjee S, Biswas A, et al. Gallic Acid Enriched Fraction of Phyllanthus emblica Potentiates Indomethacin-Induced Gastric Ulcer Healing via e-NOS-Dependent Pathway. Evid Based Complement Alternat Med. 2012; 2012: 487380.
  13. Coşkun O, Kanter M, Armutçu F, et al. Protective effects of quercetin, a flavonoid antioxidant in absolute ethanol-induced acute gastric ulcer. Eur J Gen Med. 2004; 1: 37–42.
  14. Cotelle N. Role of flavonoids in oxidative stress. Curr Top Med Chem. 2001; 1(6): 569–590.
  15. Cushnie T, Lamb A. Antimicrobial activity of flavonoids. Int J Antimicrob Agents. 2005; 26(5): 343–356.
  16. Sánchez de Medina F, Gálvez J, González M, et al. Effects of quercetin on epithelial chloride secretion. Life Sci. 1997; 61(20): 2049–2055.
  17. Drăghia AC. Histochemical and histopathological study of the gastric mucosa in the portal hypertensive gastropathy. Rom J Morphol Embryol. 2006; 47(3): 259–262.
  18. Dursun H, Bilici M, Albayrak F, et al. Antiulcer activity of fluvoxamine in rats and its effect on oxidant and antioxidant parameters in stomach tissue. BMC Gastroenterol. 2009; 9: 36.
  19. Erlund I. Review of the flavonoids quercetin, hesperetin, and naringenin. Dietary sources, bioactivities, bioavailability, and epidemiology. Nutr Res. 2004; 24(10): 851–874.
  20. Ertem D. Clinical practice: Helicobacter pylori infection in childhood. Eur J Pediatr. 2013; 172(11): 1427–1434.
  21. Farombi EO. Genotoxicity of chloroquine in rat liver cells: protective role of free radical scavengers. Cell Biol Toxicol. 2006; 22(3): 159–167.
  22. Fawcett DW. The Cell. W.B. Saunders Co, Philadelphia 1981.
  23. Fiorucci S, Antonelli E, Morelli A. Mechanism of non-steroidal anti-inflammatory drug-gastropathy. Dig Liver Dis. 2001; 33 Suppl 2: S35–S43.
  24. Fornai M, Natale G, Colucci R, et al. Mechanisms of protection by pantoprazole against NSAID-induced gastric mucosal damage. Naunyn Schmiedebergs Arch Pharmacol. 2005; 372(1): 79–87.
  25. Freitas FF, Fernandes HB, Piauilino CA, et al. Gastroprotective activity of Zanthoxylum rhoifolium Lam. in animal models. J Ethnopharmacol. 2011; 137(1): 700–708.
  26. Genta RM. Differential diagnosis of reactive gastropathy. Semin Diagn Pathol. 2005; 22(4): 273–283.
  27. Geraets L, Moonen HJJ, Brauers K, et al. Dietary flavones and flavonoles are inhibitors of poly(ADP-ribose)polymerase-1 in pulmonary epithelial cells. J Nutr. 2007; 137(10): 2190–2195.
  28. Gross M. Flavonoids and Cardiovascular Disease. Arch Physiol Biochem. 2004; 42(s1): 21–35.
  29. Heijnen CG, Haenen GR, van Acker FA, et al. Flavonoids as peroxynitrite scavengers: the role of the hydroxyl groups. Toxicol In Vitro. 2001; 15(1): 3–6.
  30. Jackson JK, Higo T, Hunter WL, et al. The antioxidants curcumin and quercetin inhibit inflammatory processes associated with arthritis. Inflamm Res. 2006; 55(4): 168–175.
  31. Kahraman A, Erkasap N, Köken T, et al. The antioxidative and antihistaminic properties of quercetin in ethanol-induced gastric lesions. Toxicology. 2003; 183(1-3): 133–142.
  32. Kanter M, Aktas C, Erboga M. Protective effects of quercetin against apoptosis and oxidative stress in streptozotocin-induced diabetic rat testis. Food Chem Toxicol. 2012; 50(3-4): 719–725.
  33. Kelly GS. Quercetin. Monograph. Altern Med Rev. 2011; 16(2): 172–194.
  34. Kim HP, Mani I, Iversen L, et al. Effects of naturally-occurring flavonoids and biflavonoids on epidermal cyclooxygenase and lipoxygenase from guinea-pigs. Prostaglandins Leukot Essent Fatty Acids. 1998; 58(1): 17–24.
  35. Knekt P, Kumpulainen J, Järvinen R, et al. Flavonoid intake and risk of chronic diseases. Am J Clin Nutr. 2002; 76(3): 560–568.
  36. Kumar V, Bhat ZA, Kumar D, et al. Gastroprotective effect of leaf extracts of Basella alba var. alba against experimental gastric ulcers in rats. Rev Bras Farmacogn. 2012; 22(3): 657–662.
  37. Lakhanpal P, Rai DK. Quercetin: A Versatile Flavonoid. Int J Med Update. 2007; 2(2).
  38. Lima CF, Fernandes-Ferreira M, Pereira-Wilson C. Phenolic compounds protect HepG2 cells from oxidative damage: relevance of glutathione levels. Life Sci. 2006; 79(21): 2056–2068.
  39. Liu JL, Du J, Fan LL, et al. Effects of quercetin on hyper-proliferation of gastric mucosal cells in rats treated with chronic oral ethanol through the reactive oxygen species-nitric oxide pathway. World J Gastroenterol. 2008; 14(20): 3242–3248.
  40. Malfertheiner P, Chan F, McColl K. Peptic ulcer disease. The Lancet. 2009; 374(9699): 1449–1461.
  41. Mamani-Matsuda M, Kauss T, Al-Kharrat A, et al. Therapeutic and preventive properties of quercetin in experimental arthritis correlate with decreased macrophage inflammatory mediators. Biochem Pharmacol. 2006; 72(10): 1304–1310.
  42. Milosavljevic T, Kostić-Milosavljević M, Jovanović I, et al. Complications of peptic ulcer disease. Dig Dis. 2011; 29(5): 491–493.
  43. Mira L, Fernandez MT, Santos M, et al. Interactions of flavonoids with iron and copper ions: a mechanism for their antioxidant activity. Free Radic Res. 2002; 36(11): 1199–1208.
  44. Miyamoto M, Haruma K. [Gastric ulcer and duodenal ulcer]. Nihon Rinsho. 2013; 71(8): 1418–1423.
  45. Moghaddam G, Sharifzadeh M, Hassanzadeh G, et al. Anti-Ulcerative Potential of Punica granatum L (Lythraceae) Hydroalcohol Fruit Peel Extract. Trop J Pharm Res. 2014; 13(7): 1093.
  46. Myhrstad MCW, Carlsen H, Nordström O, et al. Flavonoids increase the intracellular glutathione level by transactivation of the gamma-glutamylcysteine synthetase catalytical subunit promoter. Free Radic Biol Med. 2002; 32(5): 386–393.
  47. Naesdal J, Brown K. NSAID-associated adverse effects and acid control aids to prevent them: a review of current treatment options. Drug Saf. 2006; 29(2): 119–132.
  48. Pawlikowska-Pawlega B, Gruszecki WI, Misiak LE, et al. The study of the quercetin action on human erythrocyte membranes. Biochem Pharmacol. 2003; 66(4): 605–612.
  49. Perez-Vizcaino F, Duarte J, Jimenez R, et al. Antihypertensive effects of the flavonoid quercetin. Pharmacol Rep. 2009; 61(1): 67–75.
  50. Polat B, Albayrak Y, Suleyman B, et al. Antiulcerative effect of dexmedetomidine on indomethacin-induced gastric ulcer in rats. Pharmacol Rep. 2011; 63(2): 518–526.
  51. Ramakrishnan K, Salinas RC. Peptic ulcer disease. Am Fam Physician. 2007; 76(7): 1005–1012.
  52. Repetto MG, Llesuy SF. Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz J Med Biol Res. 2002; 35(5): 523–534.
  53. Robaszkiewicz A, Balcerczyk A, Bartosz G. Antioxidative and prooxidative effects of quercetin on A549 cells. Cell Biol Int. 2007; 31(10): 1245–1250.
  54. Russo M, Spagnuolo C, Tedesco I, et al. The flavonoid quercetin in disease prevention and therapy: facts and fancies. Biochem Pharmacol. 2012; 83(1): 6–15.
  55. Sairam K, Rao CV, Goel RK. Effect of Convolvulus pluricaulis Chois on gastric ulceration and secretion in rats. Indian J Exp Biol. 2001; 39(4): 350–354.
  56. Sandhar HK, Kumar B, Prasher S, et al. Review of Phytochemistry and Pharmacology of Flavonoids. Int Pharmaceutica Sciencia. 2011; 1: 25–41.
  57. Seckin Y, Harputluoglu MMM, Batcioglu K, et al. Gastric tissue oxidative changes in portal hypertension and cirrhosis. Dig Dis Sci. 2007; 52(5): 1154–1158.
  58. Sener G, Paskaloglu K, Ayanoglu-dülger G. Protective effect of increasing doses of famotidine, omeprazole, lansoprazole, and melatonin against ethanol-induced gastric damage in rats. Indian J Pharmacol. 2004; 36: 171–174.
  59. Sumbul S, Ahmad MA, Mohd A, et al. Role of phenolic compounds in peptic ulcer: An overview. J Pharm Bioallied Sci. 2011; 3(3): 361–367.
  60. Surapaneni SR, Reddy AB. The Perforation-Operation time Interval; An Important Mortality Indicator in Peptic Ulcer Perforation. J Clin Diagn Res. 2013; 7(5): 880–882.
  61. Tang RS, Chan FKL. Therapeutic management of recurrent peptic ulcer disease. Drugs. 2012; 72(12): 1605–1616.
  62. Terao J. Dietary Flavonoids as Antioxidants. In: Yoshikawa T (ed.). Food Factors for Health Promotion. Forum Nutr. Karger, Basel 2009: pp. 87–94.
  63. Thorsen K, Søreide JA, Søreide K. Epidemiology of perforated peptic ulcer: Age- and gender-adjusted analysis of incidence and mortality. World J Gastroenterol. 2013; 19(3): 347.
  64. Toki M, Aoki K, Katsumi N, et al. [NSAID and its effect on prostaglandin]. Nihon Rinsho. 2007; 65(10): 1807–1811.
  65. Villegas I, La Casa C, de la Lastra CA, et al. Mucosal damage induced by preferential COX-1 and COX-2 inhibitors: role of prostaglandins and inflammatory response. Life Sci. 2004; 74(7): 873–884.
  66. Villegas I, Martín MJ, La Casa C, et al. Effects of oxicam inhibitors of cyclooxygenase on oxidative stress generation in rat gastric mucosa. A comparative study. Free Radic Res. 2002; 36(7): 769–777.
  67. Webster-Gandy J, Madden A, Holdsworth M. Oxford handbook of nutrition and dietetics. 2nd ed. Oxford University Press, Oxford 2012.
  68. Wiczkowski W, Nèmeth K, Buciński A, et al. Bioavailability of Quercetin from Flesh Scales and Dry Skin of Onion in Rats. Pol J Food Nutr Sci. 2003; 12: 95–99.
  69. Wu LX, Guo CX, Chen WQ, et al. Inhibition of the organic anion-transporting polypeptide 1B1 by quercetin: an in vitro and in vivo assessment. Br J Clin Pharmacol. 2012; 73(5): 750–757.
  70. Yamazaki Y, Ueda T, Kohli Y, et al. Importance of acidic mucin secretions by foveolar and mucous neck cells of rat fundic mucosa as the defence mechanisms against HCl as revealed by fasting. Eur J Histochem. 1992; 36(2): 161–176.
  71. Yang JH, Hsia TC, Kuo HM, et al. Inhibition of lung cancer cell growth by quercetin glucuronides via G2/M arrest and induction of apoptosis. Drug Metab Dispos. 2006; 34(2): 296–304.
  72. Yao LH, Jiang YM, Shi J, et al. Flavonoids in food and their health benefits. Plant Foods Hum Nutr. 2004; 59(3): 113–122.
  73. Ye B, Zhou PY, Jia M, et al. Absence of NF-κB subunit p50 ameliorates cold immobilization stress-induced gastric ulcers. Biochem Biophys Res Commun. 2013; 434(3): 547–551.
  74. Zahorodnyĭ MI. [Effect of quercetin on sodium diclofenac-induced ulceration]. Lik Sprava. 2003(1): 96–99.

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