open access

Vol 75, No 4 (2016)
ORIGINAL ARTICLES
Published online: 2016-02-24
Submitted: 2015-10-31
Accepted: 2016-01-07
Get Citation

Histochemical study of the oesophagus in the chukar partridge (Alectoris chukar) embryo

B. Shojaei, S. Hashemnia, R. Eslami Rad
DOI: 10.5603/FM.a2016.0011
·
Pubmed: 26916205
·
Folia Morphol 2016;75(4):474-480.

open access

Vol 75, No 4 (2016)
ORIGINAL ARTICLES
Published online: 2016-02-24
Submitted: 2015-10-31
Accepted: 2016-01-07

Abstract

Study of the histogenesis of different organs is a useful laboratory method which helps us achieve accurate basic information about organ development during the embryonic period. It also reveals histological differences of each organ in different species. This research was carried out to study the histogenesis of the oesophagus of chukar partridge. For this purpose, the embryonated eggs were placed in the incubator and the embryos were collected between the 5th to 24th days of incubation period. The specimens were fixed in Bouin’s solution, and routine histotechnique processes were performed. The slides were finally stained with haematoxylin-eosin, Periodic Acid Schiff, Alcian Blue and Masson Trichrome, and the developmental changes of the oesophagus during the embryonic period were studied by light microscope. The four layers of oesophagus, including, the mucosa, submucosa, tunica muscularis and advantitia/serosa, both in cervical and thoracic oesophagus, were studied. During days 5 to 24 of incubation, developmental events in the oesophagus such as changes in the oesophageal epithelium, formation of muscularis mucosae and tunica muscularis, development of the mucous glands and the type of their secretion, were observed. Finally the results were compared with those of other studied avian species and the similarities and differences were discussed.

Abstract

Study of the histogenesis of different organs is a useful laboratory method which helps us achieve accurate basic information about organ development during the embryonic period. It also reveals histological differences of each organ in different species. This research was carried out to study the histogenesis of the oesophagus of chukar partridge. For this purpose, the embryonated eggs were placed in the incubator and the embryos were collected between the 5th to 24th days of incubation period. The specimens were fixed in Bouin’s solution, and routine histotechnique processes were performed. The slides were finally stained with haematoxylin-eosin, Periodic Acid Schiff, Alcian Blue and Masson Trichrome, and the developmental changes of the oesophagus during the embryonic period were studied by light microscope. The four layers of oesophagus, including, the mucosa, submucosa, tunica muscularis and advantitia/serosa, both in cervical and thoracic oesophagus, were studied. During days 5 to 24 of incubation, developmental events in the oesophagus such as changes in the oesophageal epithelium, formation of muscularis mucosae and tunica muscularis, development of the mucous glands and the type of their secretion, were observed. Finally the results were compared with those of other studied avian species and the similarities and differences were discussed.

Get Citation

Keywords

Aves, gastrointestinal, histology, development, embryology

About this article
Title

Histochemical study of the oesophagus in the chukar partridge (Alectoris chukar) embryo

Journal

Folia Morphologica

Issue

Vol 75, No 4 (2016)

Pages

474-480

Published online

2016-02-24

DOI

10.5603/FM.a2016.0011

Pubmed

26916205

Bibliographic record

Folia Morphol 2016;75(4):474-480.

Keywords

Aves
gastrointestinal
histology
development
embryology

Authors

B. Shojaei
S. Hashemnia
R. Eslami Rad

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl