open access

Vol 75, No 4 (2016)
ORIGINAL ARTICLES
Published online: 2016-03-17
Submitted: 2015-09-15
Accepted: 2015-11-10
Get Citation

Cloning and expression of cold-inducible RNA binding protein in domestic yak (Bos grunniens)

K. Yang, Q. Zhang, Z. Wen, Y. Pan, S. Yu, J. He, X. Yang, P. Liu, Y. Cui
DOI: 10.5603/FM.a2016.0015
·
Pubmed: 27830872
·
Folia Morphol 2016;75(4):460-466.

open access

Vol 75, No 4 (2016)
ORIGINAL ARTICLES
Published online: 2016-03-17
Submitted: 2015-09-15
Accepted: 2015-11-10

Abstract

Cold-inducible RNA binding protein (CIRP) is over-expressed during cold and many other stresses, and could regulate the adaptation to hypothermia. In the present investigation, the objective was to determine the expression of CIRP in adult yak heart, liver, spleen, lung, kidney, brain, ovary, testis and skin by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry from mRNA and protein levels. The CIRP open reading frame encoding was cloned from the domestic yak brain. Results of RT-PCR and Western blot showed the high expression level of CIRP in the heart, kidney, brain, testis and skin, and the lower expression level of CIRP in the lung. Immunohistochemical staining showed CIRP was expressed in the nucleus of neuronal cells, spermatogonia, primary spermatocytes and epidermal cells, and in the cytoplasm of the residual tissues. These observations may provide new data to understand and further study the important role of CIRP protein in the plateau adaptation of the domestic yak on long-term evolution. 

Abstract

Cold-inducible RNA binding protein (CIRP) is over-expressed during cold and many other stresses, and could regulate the adaptation to hypothermia. In the present investigation, the objective was to determine the expression of CIRP in adult yak heart, liver, spleen, lung, kidney, brain, ovary, testis and skin by relative quantitative real time polymerase chain reaction (RT-PCR), Western blot and immunohistochemistry from mRNA and protein levels. The CIRP open reading frame encoding was cloned from the domestic yak brain. Results of RT-PCR and Western blot showed the high expression level of CIRP in the heart, kidney, brain, testis and skin, and the lower expression level of CIRP in the lung. Immunohistochemical staining showed CIRP was expressed in the nucleus of neuronal cells, spermatogonia, primary spermatocytes and epidermal cells, and in the cytoplasm of the residual tissues. These observations may provide new data to understand and further study the important role of CIRP protein in the plateau adaptation of the domestic yak on long-term evolution. 

Get Citation

Keywords

yak, cold-inducible RNA binding protein, expression, adaptation, RT-PCR, Western blot, immunohistochemistry

About this article
Title

Cloning and expression of cold-inducible RNA binding protein in domestic yak (Bos grunniens)

Journal

Folia Morphologica

Issue

Vol 75, No 4 (2016)

Pages

460-466

Published online

2016-03-17

DOI

10.5603/FM.a2016.0015

Pubmed

27830872

Bibliographic record

Folia Morphol 2016;75(4):460-466.

Keywords

yak
cold-inducible RNA binding protein
expression
adaptation
RT-PCR
Western blot
immunohistochemistry

Authors

K. Yang
Q. Zhang
Z. Wen
Y. Pan
S. Yu
J. He
X. Yang
P. Liu
Y. Cui

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