open access

Vol 74, No 4 (2015)
ORIGINAL ARTICLES
Published online: 2015-11-27
Submitted: 2015-05-12
Accepted: 2015-07-15
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Long term effects of maternal protein restriction on postnatal lung alveoli development of rat offspring

S. A. Farid, O. M. Mahmoud, N. A. Salem, G. Abdel-Alrahman, G. A. Hafez
DOI: 10.5603/FM.2015.0111
·
Pubmed: 26620509
·
Folia Morphol 2015;74(4):479-485.

open access

Vol 74, No 4 (2015)
ORIGINAL ARTICLES
Published online: 2015-11-27
Submitted: 2015-05-12
Accepted: 2015-07-15

Abstract

Poor nutrition of women during pregnancy causes reduction in foetal growth and can adversely affect the development of the foetal lungs. The purpose of the present study was to assess the effects of maternal protein restriction on the postnatal lung development in neonatal period, and on lung structure in adult rat offspring. Female virgin Sprague-Dawley albino rats (more than 200 g) were used. One male rat was introduced into a cage with one female for matting. Once the pregnancy was confirmed, pregnant rats were divided into two main groups; each consists of 6 female as follow: 1 — normally nourished group; 2 — protein deficient group. After delivery, offspring were subdivided into three groups: 1 day after delivery, 2 weeks and 2 months postnatal. Rat body and lung weight were recorded and ratio of lung weight to body weight was assessed. Total plasma protein and serum albumin were assessed for all groups. Lung tissue stained with H&E for histological and morphometric analysis. Immunohistochemistry was performed to evaluate the number of cells positive for pulmonary surfactant protein A. Our results showed that protein restriction interfere with neonatal and postnatal lung development resulting in morphological and morphometric changes of normal lung development. We concluded that protein deficiency lead to developmental retardation of lung.

Abstract

Poor nutrition of women during pregnancy causes reduction in foetal growth and can adversely affect the development of the foetal lungs. The purpose of the present study was to assess the effects of maternal protein restriction on the postnatal lung development in neonatal period, and on lung structure in adult rat offspring. Female virgin Sprague-Dawley albino rats (more than 200 g) were used. One male rat was introduced into a cage with one female for matting. Once the pregnancy was confirmed, pregnant rats were divided into two main groups; each consists of 6 female as follow: 1 — normally nourished group; 2 — protein deficient group. After delivery, offspring were subdivided into three groups: 1 day after delivery, 2 weeks and 2 months postnatal. Rat body and lung weight were recorded and ratio of lung weight to body weight was assessed. Total plasma protein and serum albumin were assessed for all groups. Lung tissue stained with H&E for histological and morphometric analysis. Immunohistochemistry was performed to evaluate the number of cells positive for pulmonary surfactant protein A. Our results showed that protein restriction interfere with neonatal and postnatal lung development resulting in morphological and morphometric changes of normal lung development. We concluded that protein deficiency lead to developmental retardation of lung.

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Keywords

lung development, protein restriction, rat

About this article
Title

Long term effects of maternal protein restriction on postnatal lung alveoli development of rat offspring

Journal

Folia Morphologica

Issue

Vol 74, No 4 (2015)

Pages

479-485

Published online

2015-11-27

DOI

10.5603/FM.2015.0111

Pubmed

26620509

Bibliographic record

Folia Morphol 2015;74(4):479-485.

Keywords

lung development
protein restriction
rat

Authors

S. A. Farid
O. M. Mahmoud
N. A. Salem
G. Abdel-Alrahman
G. A. Hafez

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