open access

Vol 74, No 3 (2015)
ORIGINAL ARTICLES
Published online: 2015-09-02
Submitted: 2014-10-19
Accepted: 2014-12-10
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Efficiency of selenium in attenuating epididymal histopathological changes in hypercholesterolaemic adult rat

F. A. Abdel Gawad, E. A.A. El-Shaarawy, A. A. Rizk, W. M. Sayed, R. A. Emam
DOI: 10.5603/FM.2015.0045
·
Pubmed: 26339809
·
Folia Morphol 2015;74(3):295-302.

open access

Vol 74, No 3 (2015)
ORIGINAL ARTICLES
Published online: 2015-09-02
Submitted: 2014-10-19
Accepted: 2014-12-10

Abstract

Background: Studies on sperm maturation, epididymal histology, or epididymal tubule physiology are significant parts in reproductive researches. The present study was aimed to evaluate the effect of induced hypercholesterolaemia on the epididymis of adult albino rats and to clarify the possible protective role of selenium.

Materials and methods: Forty adult albino Wistar rats were divided into four groups; untreated control group (group I), sham control (group II), group with induced hypercholesterolaemia (group III), group with induced hypercholesterolaemia treated with selenium 0.25 mg/kg/day (group IV).

Results: Histological and ultrastructural examination of the epididymal epithelial cells of hypercholesterolaemic rats (group III) showed loss of cilia with many vacuolations, fatty degenerative changes and increased collagen fibres. Morphometrically significant increase (p < 0.0001) in the per cent area of collagen fibres with no significant change in the optical density of periodic acid Schiff reaction (p > 0.05). Selenium treated group (group IV) produced marked improvement in histological, ultrastructural and morphometric results as compared with group III.

Conclusions: It could be concluded that hypercholesterolaemia produced deleterious effects to the epididymis and selenium could attenuate these effects.

Abstract

Background: Studies on sperm maturation, epididymal histology, or epididymal tubule physiology are significant parts in reproductive researches. The present study was aimed to evaluate the effect of induced hypercholesterolaemia on the epididymis of adult albino rats and to clarify the possible protective role of selenium.

Materials and methods: Forty adult albino Wistar rats were divided into four groups; untreated control group (group I), sham control (group II), group with induced hypercholesterolaemia (group III), group with induced hypercholesterolaemia treated with selenium 0.25 mg/kg/day (group IV).

Results: Histological and ultrastructural examination of the epididymal epithelial cells of hypercholesterolaemic rats (group III) showed loss of cilia with many vacuolations, fatty degenerative changes and increased collagen fibres. Morphometrically significant increase (p < 0.0001) in the per cent area of collagen fibres with no significant change in the optical density of periodic acid Schiff reaction (p > 0.05). Selenium treated group (group IV) produced marked improvement in histological, ultrastructural and morphometric results as compared with group III.

Conclusions: It could be concluded that hypercholesterolaemia produced deleterious effects to the epididymis and selenium could attenuate these effects.

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Keywords

hypercholesterolaemia, epididymis, selenium, histopathology

About this article
Title

Efficiency of selenium in attenuating epididymal histopathological changes in hypercholesterolaemic adult rat

Journal

Folia Morphologica

Issue

Vol 74, No 3 (2015)

Pages

295-302

Published online

2015-09-02

DOI

10.5603/FM.2015.0045

Pubmed

26339809

Bibliographic record

Folia Morphol 2015;74(3):295-302.

Keywords

hypercholesterolaemia
epididymis
selenium
histopathology

Authors

F. A. Abdel Gawad
E. A.A. El-Shaarawy
A. A. Rizk
W. M. Sayed
R. A. Emam

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