open access

Vol 74, No 2 (2015)
ORIGINAL ARTICLES
Published online: 2015-05-28
Submitted: 2014-09-30
Accepted: 2014-11-10
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Immunohistochemical evidence of the co-localisation of cocaine and amphetamine regulatory peptide with neuronal isoform of nitric oxide synthase, vasoactive intestinal peptide and galanin within the circular muscle layer of the human caecum

M. Bulc, S. Gonkowski, P. Landowski, B. Kamińska, J. Całka
DOI: 10.5603/FM.2015.0028
·
Pubmed: 26050803
·
Folia Morphol 2015;74(2):176-182.

open access

Vol 74, No 2 (2015)
ORIGINAL ARTICLES
Published online: 2015-05-28
Submitted: 2014-09-30
Accepted: 2014-11-10

Abstract

The enteric nervous system consists of about one hundred million of neurons. In big mammals (including humans) intestinal enteric neuronal cells are grouped into three types of intramural ganglia located within myenteric, as well as outer and inner submucosal plexuses, which are connected by numerous nerve fibres. Both nerve fibres and cell bodies located in the gastrointestinal tract utilise a broad spectrum of active substances. One of them is cocaine- and amphetamine-regulated transcript peptide (CART). The goal of the current study was to determinate the distribution and degree of co-localisation of CART with substances taking part in intestinal motor activity by double labelling immunofluorescence technique. During the study CART-, neuronal isoform of nitric oxide synthase (nNOS)-, vasoactive intestinal peptide (VIP)- and/or galanin (GAL) — like immunoreactive (LI) nerve fibres in the circular muscle layer of the human caecum were observed in all patients studied. The degree of co-localisation of particular substances with CART depended on their type. The majority of CART-LI fibres contained simultaneously nNOS, slightly lower degree of co-localisation was observed in the case of the VIP, while simultaneously CART- and GAL-positive nerve fibres were observed less often.

Abstract

The enteric nervous system consists of about one hundred million of neurons. In big mammals (including humans) intestinal enteric neuronal cells are grouped into three types of intramural ganglia located within myenteric, as well as outer and inner submucosal plexuses, which are connected by numerous nerve fibres. Both nerve fibres and cell bodies located in the gastrointestinal tract utilise a broad spectrum of active substances. One of them is cocaine- and amphetamine-regulated transcript peptide (CART). The goal of the current study was to determinate the distribution and degree of co-localisation of CART with substances taking part in intestinal motor activity by double labelling immunofluorescence technique. During the study CART-, neuronal isoform of nitric oxide synthase (nNOS)-, vasoactive intestinal peptide (VIP)- and/or galanin (GAL) — like immunoreactive (LI) nerve fibres in the circular muscle layer of the human caecum were observed in all patients studied. The degree of co-localisation of particular substances with CART depended on their type. The majority of CART-LI fibres contained simultaneously nNOS, slightly lower degree of co-localisation was observed in the case of the VIP, while simultaneously CART- and GAL-positive nerve fibres were observed less often.

Get Citation

Keywords

cocaine- and amphetamine-regulated peptide, neuronal isoform of nitric oxide synthase vasoactive intestinal peptide, galanin, caecum, human

About this article
Title

Immunohistochemical evidence of the co-localisation of cocaine and amphetamine regulatory peptide with neuronal isoform of nitric oxide synthase, vasoactive intestinal peptide and galanin within the circular muscle layer of the human caecum

Journal

Folia Morphologica

Issue

Vol 74, No 2 (2015)

Pages

176-182

Published online

2015-05-28

DOI

10.5603/FM.2015.0028

Pubmed

26050803

Bibliographic record

Folia Morphol 2015;74(2):176-182.

Keywords

cocaine- and amphetamine-regulated peptide
neuronal isoform of nitric oxide synthase vasoactive intestinal peptide
galanin
caecum
human

Authors

M. Bulc
S. Gonkowski
P. Landowski
B. Kamińska
J. Całka

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