open access

Vol 74, No 2 (2015)
REVIEW ARTICLES
Published online: 2015-05-28
Submitted: 2014-09-24
Accepted: 2014-10-02
Get Citation

Essential and current methods for a practical approach to comparative neuropathology

D. De Biase, O. Paciello
DOI: 10.5603/FM.2015.0024
·
Pubmed: 26050799
·
Folia Morphol 2015;74(2):137-149.

open access

Vol 74, No 2 (2015)
REVIEW ARTICLES
Published online: 2015-05-28
Submitted: 2014-09-24
Accepted: 2014-10-02

Abstract

The understanding of mechanisms that provoke neurological diseases in humans and in animals has progressed rapidly in recent years, mainly due to the advent of new research instruments and our increasing liability to assemble large, complex data sets acquired across several approaches into an integrated representation of neural function at the molecular, cellular, and systemic levels. Nevertheless, morphology always represents the essential approaches that are crucial for any kind of interpretation of the lesions or to explain new molecular pathways in the diseases. This mini-review has been designed to illustrate the newest and also well-established principal methods for the nervous tissue collection and processing as well as to describe the histochemical and immunohistochemical staining tools that are currently most suitable for a neuropathological assessment of the central nervous system. We also present the results of our neuropathological studies covering material from 170 cases belonging to 10 different species of mammals. Specific topics briefly addressed in this paper provide a technical and practical guide not only for researchers that daily focus their effort on neuropathology studies, but also to pathologists who occasionally have to approach to nervous tissue evaluation to answer questions about neuropathology issues.

Abstract

The understanding of mechanisms that provoke neurological diseases in humans and in animals has progressed rapidly in recent years, mainly due to the advent of new research instruments and our increasing liability to assemble large, complex data sets acquired across several approaches into an integrated representation of neural function at the molecular, cellular, and systemic levels. Nevertheless, morphology always represents the essential approaches that are crucial for any kind of interpretation of the lesions or to explain new molecular pathways in the diseases. This mini-review has been designed to illustrate the newest and also well-established principal methods for the nervous tissue collection and processing as well as to describe the histochemical and immunohistochemical staining tools that are currently most suitable for a neuropathological assessment of the central nervous system. We also present the results of our neuropathological studies covering material from 170 cases belonging to 10 different species of mammals. Specific topics briefly addressed in this paper provide a technical and practical guide not only for researchers that daily focus their effort on neuropathology studies, but also to pathologists who occasionally have to approach to nervous tissue evaluation to answer questions about neuropathology issues.

Get Citation

Keywords

histology, brain, histochemistry, immunohistochemistry, necropsy, nervous tissue fixation procedures, nervous tissue artefacts

About this article
Title

Essential and current methods for a practical approach to comparative neuropathology

Journal

Folia Morphologica

Issue

Vol 74, No 2 (2015)

Pages

137-149

Published online

2015-05-28

DOI

10.5603/FM.2015.0024

Pubmed

26050799

Bibliographic record

Folia Morphol 2015;74(2):137-149.

Keywords

histology
brain
histochemistry
immunohistochemistry
necropsy
nervous tissue fixation procedures
nervous tissue artefacts

Authors

D. De Biase
O. Paciello

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