open access

Vol 74, No 3 (2015)
ORIGINAL ARTICLES
Published online: 2015-09-02
Submitted: 2014-10-09
Accepted: 2014-11-11
Get Citation

Volume calculation of the cattle (Bos taurus L.) and the water buffalo (Bos bubalis L.) metapodia with stereologic method

N. G. Ince, G. Pazvant, V. Onar, A. Chrószcz, E. Pasicka
DOI: 10.5603/FM.2015.0050
·
Pubmed: 26339814
·
Folia Morphol 2015;74(3):335-339.

open access

Vol 74, No 3 (2015)
ORIGINAL ARTICLES
Published online: 2015-09-02
Submitted: 2014-10-09
Accepted: 2014-11-11

Abstract

In this study, stereological volume estimations using 26 cattle metapodia (26 metacarpal and 26 metatarsal bones) and 8 water buffalo metapodia (8 metacarpal and 8 metatarsal bones) were made. For this purpose metapodia were parallel sectioned at 1 cm intervals according to Cavalieri principle. Grids with 0.4 cm probe intervals were superimposed on top of these sections and the matching points were counted. All of the bone structures and medullar cavity volumes were calculated with the data obtained from a formulation (V = t × a(p) × ΣP) as a spreadsheet using Microsoft Excel® Windows XP. In addition, percent ratio of this volume to whole bone volume was calculated. The mean ratio of bone marrow space to whole bone structure volume equals 15% in all of the cattle and buffalos. The difference between whole bone volumes of cattle and water buffalo was significant (p < 0.05) while the difference in volume of medullary cavity (cavum medullare) was not significantly different between the two investigated species. The aim of current study is to present a new method that can be used for the volumes calculation of whole bones and medullary cavity in metapodial bones and their percentages.  

Abstract

In this study, stereological volume estimations using 26 cattle metapodia (26 metacarpal and 26 metatarsal bones) and 8 water buffalo metapodia (8 metacarpal and 8 metatarsal bones) were made. For this purpose metapodia were parallel sectioned at 1 cm intervals according to Cavalieri principle. Grids with 0.4 cm probe intervals were superimposed on top of these sections and the matching points were counted. All of the bone structures and medullar cavity volumes were calculated with the data obtained from a formulation (V = t × a(p) × ΣP) as a spreadsheet using Microsoft Excel® Windows XP. In addition, percent ratio of this volume to whole bone volume was calculated. The mean ratio of bone marrow space to whole bone structure volume equals 15% in all of the cattle and buffalos. The difference between whole bone volumes of cattle and water buffalo was significant (p < 0.05) while the difference in volume of medullary cavity (cavum medullare) was not significantly different between the two investigated species. The aim of current study is to present a new method that can be used for the volumes calculation of whole bones and medullary cavity in metapodial bones and their percentages.  

Get Citation

Keywords

metapodium, stereology, bone marrow

About this article
Title

Volume calculation of the cattle (Bos taurus L.) and the water buffalo (Bos bubalis L.) metapodia with stereologic method

Journal

Folia Morphologica

Issue

Vol 74, No 3 (2015)

Pages

335-339

Published online

2015-09-02

DOI

10.5603/FM.2015.0050

Pubmed

26339814

Bibliographic record

Folia Morphol 2015;74(3):335-339.

Keywords

metapodium
stereology
bone marrow

Authors

N. G. Ince
G. Pazvant
V. Onar
A. Chrószcz
E. Pasicka

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