open access

Vol 74, No 2 (2015)
ORIGINAL ARTICLES
Published online: 2015-05-28
Submitted: 2014-07-23
Accepted: 2014-10-13
Get Citation

Comparison of formaldehyde and methanol fixatives used in the detection of ion channel proteins in isolated rat ventricular myocytes by immunofluorescence labelling and confocal microscopy

T. T. Yamanushi, M. R. Boyett, Y. Yamamoto, H. Ohsaki, E. Hirakawa, H. Dobrzynski
DOI: 10.5603/FM.2015.0041
·
Pubmed: 26050816
·
Folia Morphol 2015;74(2):258-261.

open access

Vol 74, No 2 (2015)
ORIGINAL ARTICLES
Published online: 2015-05-28
Submitted: 2014-07-23
Accepted: 2014-10-13

Abstract

In this study, a fixation protocol using a 10% neutral buffered formalin (FA) solution and another protocol using a methanol (MeOH) solution were compared for detection of ion channels, Kv1.5, Kv4.2, Cav1.2, Kir6.2, Nav1.5 and Nav1.1 in rat myocytes by immunolabelling. Kv1.5 and Kv4.2 at intercalated discs and Cav1.2 at transverse tubules were not detected by FA but were detected by MeOH. Kir6.2 at transverse tubules and Nav1.5 at sarcolemma were detected by FA but not by MeOH. It is suggested that both FA and MeOH fixation protocols should be used for the detection of cardiac ion channels by immunolabelling

Abstract

In this study, a fixation protocol using a 10% neutral buffered formalin (FA) solution and another protocol using a methanol (MeOH) solution were compared for detection of ion channels, Kv1.5, Kv4.2, Cav1.2, Kir6.2, Nav1.5 and Nav1.1 in rat myocytes by immunolabelling. Kv1.5 and Kv4.2 at intercalated discs and Cav1.2 at transverse tubules were not detected by FA but were detected by MeOH. Kir6.2 at transverse tubules and Nav1.5 at sarcolemma were detected by FA but not by MeOH. It is suggested that both FA and MeOH fixation protocols should be used for the detection of cardiac ion channels by immunolabelling

Get Citation

Keywords

heart, immunocytochemistry, fixation, cross-linker, coagulant, formalin

About this article
Title

Comparison of formaldehyde and methanol fixatives used in the detection of ion channel proteins in isolated rat ventricular myocytes by immunofluorescence labelling and confocal microscopy

Journal

Folia Morphologica

Issue

Vol 74, No 2 (2015)

Pages

258-261

Published online

2015-05-28

DOI

10.5603/FM.2015.0041

Pubmed

26050816

Bibliographic record

Folia Morphol 2015;74(2):258-261.

Keywords

heart
immunocytochemistry
fixation
cross-linker
coagulant
formalin

Authors

T. T. Yamanushi
M. R. Boyett
Y. Yamamoto
H. Ohsaki
E. Hirakawa
H. Dobrzynski

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl