open access

Vol 74, No 2 (2015)
ORIGINAL ARTICLES
Published online: 2015-05-28
Submitted: 2014-06-12
Accepted: 2014-09-17
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Collagen types I and II distribution: a relevant indicator for the functional properties of articular cartilage in immobilised and remobilised rabbit knee joints

A. M. Aldahmash, A. F. El Fouhil, R. A. Mohamed, A. M. Ahmed, M. Atteya, S. A. Al Sharawy, R. A. Qureshi
DOI: 10.5603/FM.2015.0027
·
Pubmed: 26050802
·
Folia Morphol 2015;74(2):169-175.

open access

Vol 74, No 2 (2015)
ORIGINAL ARTICLES
Published online: 2015-05-28
Submitted: 2014-06-12
Accepted: 2014-09-17

Abstract

The objective of the present work was to study changes in collagen type I and type II distribution in the articular cartilage of immobilised and remobilised rabbit knee joints. Twenty-four adult male rabbits were divided into three groups. One of the groups was a control group with free movement. The right knee joints of animals of the other two groups were immobilised for 4 weeks, followed by a period of 10 weeks of remobilisation for animals of group 3. Collagen type I and type II in the articular cartilage of tibial medial condyle of the right knee joint were estimated immunohistochemically in all groups. A degenerative process was evident after 4 weeks of immobilisation of rabbit knee joint leading to a partial shift in the density of collagen composition from type II to type I. After a period of 10 weeks of remobilisation, regenerative processes, evidenced by a restoration of collagen type II to normal, proceeded on top of degenerative processes, evidenced by the significantly higher content of collagen type I compared with normal. The present study pointed to the importance of assessment of collagen distribution as a relevant indicator for the functional properties of articular cartilage in immobilised and remobilised joints.

Abstract

The objective of the present work was to study changes in collagen type I and type II distribution in the articular cartilage of immobilised and remobilised rabbit knee joints. Twenty-four adult male rabbits were divided into three groups. One of the groups was a control group with free movement. The right knee joints of animals of the other two groups were immobilised for 4 weeks, followed by a period of 10 weeks of remobilisation for animals of group 3. Collagen type I and type II in the articular cartilage of tibial medial condyle of the right knee joint were estimated immunohistochemically in all groups. A degenerative process was evident after 4 weeks of immobilisation of rabbit knee joint leading to a partial shift in the density of collagen composition from type II to type I. After a period of 10 weeks of remobilisation, regenerative processes, evidenced by a restoration of collagen type II to normal, proceeded on top of degenerative processes, evidenced by the significantly higher content of collagen type I compared with normal. The present study pointed to the importance of assessment of collagen distribution as a relevant indicator for the functional properties of articular cartilage in immobilised and remobilised joints.

Get Citation

Keywords

collagen type I, collagen type II, immobilised knee, immunohistochemistry

About this article
Title

Collagen types I and II distribution: a relevant indicator for the functional properties of articular cartilage in immobilised and remobilised rabbit knee joints

Journal

Folia Morphologica

Issue

Vol 74, No 2 (2015)

Pages

169-175

Published online

2015-05-28

DOI

10.5603/FM.2015.0027

Pubmed

26050802

Bibliographic record

Folia Morphol 2015;74(2):169-175.

Keywords

collagen type I
collagen type II
immobilised knee
immunohistochemistry

Authors

A. M. Aldahmash
A. F. El Fouhil
R. A. Mohamed
A. M. Ahmed
M. Atteya
S. A. Al Sharawy
R. A. Qureshi

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