open access

Vol 73, No 4 (2014)
ORIGINAL ARTICLES
Published online: 2014-11-28
Submitted: 2014-05-05
Accepted: 2014-05-24
Get Citation

Effect of prenatal exposure to bisphenol a on the vagina of albino rats: immunohistochemical and ultrastructural study

R. A.M. Ahmed, T. A. ElGhamrawy, E.E. A. Salama
DOI: 10.5603/FM.2014.0061
·
Folia Morphol 2014;73(4):399-408.

open access

Vol 73, No 4 (2014)
ORIGINAL ARTICLES
Published online: 2014-11-28
Submitted: 2014-05-05
Accepted: 2014-05-24

Abstract

Background: Bisphenol-A (BPA) is an industrial chemical, used to manufacture polycarbonate and numerous plastic articles. It has been found to cause biological effects, mimic that of oestrogen. It belongs to a group of chemicals termed “endocrine disruptors” able to disrupt the chemical messenger system in the body. Aim of the study was to demonstrate the biological effects of BPA on the vagina of female rats, with the prediction of the neoplastic changes in relation to its potential impact.

Materials and methods: Sprague-Dawley gravid dams were divided into three groups (10 per group): G1 — control group had an equivalent volume of sesame oil to that taken in the treated groups, G2 — group was administered by gavage 0.1 mg BPA/kg body weight (low-dose group) per day, and G3 — group was administered 50 mg BPA/kg body weight (high-dose group) per day, dissolved in sesame oil. Treatment was carried out on gestation days 10 through 20. The female offsprings of each group were weaned at day 21 and the vagina was dissected when became 3 months old for histological, immunohistochemical analysis (for detection of oestrogen receptors a [ERa], and the proliferation marker Ki-67), and ultrastructural study.

Results: The low dose group showed degeneration of the epithelial lining with focal patches of decreased epithelial layers. The high dose group revealed cytoplasmic hydropic degeneration, and the pyknotic nuclei of epithelial cells. Oestrogen receptors demonstrated a significant decrease of positive cells in low dose treated group and this decrease markedly accentuated in the high dose one. Positive nuclei for Ki-67 were markedly increased with increasing doses of BPA. Electron microscopic study revealed cytoplasmic degeneration, vacuolation and mitochondrial degeneration in both treated groups.

Conclusions: BPA showed an obvious mix of degenerative and proliferative histological changes and clear damage of the cellular organelles. This stressful condition may predispose to neoplastic changes of the vagina.

Abstract

Background: Bisphenol-A (BPA) is an industrial chemical, used to manufacture polycarbonate and numerous plastic articles. It has been found to cause biological effects, mimic that of oestrogen. It belongs to a group of chemicals termed “endocrine disruptors” able to disrupt the chemical messenger system in the body. Aim of the study was to demonstrate the biological effects of BPA on the vagina of female rats, with the prediction of the neoplastic changes in relation to its potential impact.

Materials and methods: Sprague-Dawley gravid dams were divided into three groups (10 per group): G1 — control group had an equivalent volume of sesame oil to that taken in the treated groups, G2 — group was administered by gavage 0.1 mg BPA/kg body weight (low-dose group) per day, and G3 — group was administered 50 mg BPA/kg body weight (high-dose group) per day, dissolved in sesame oil. Treatment was carried out on gestation days 10 through 20. The female offsprings of each group were weaned at day 21 and the vagina was dissected when became 3 months old for histological, immunohistochemical analysis (for detection of oestrogen receptors a [ERa], and the proliferation marker Ki-67), and ultrastructural study.

Results: The low dose group showed degeneration of the epithelial lining with focal patches of decreased epithelial layers. The high dose group revealed cytoplasmic hydropic degeneration, and the pyknotic nuclei of epithelial cells. Oestrogen receptors demonstrated a significant decrease of positive cells in low dose treated group and this decrease markedly accentuated in the high dose one. Positive nuclei for Ki-67 were markedly increased with increasing doses of BPA. Electron microscopic study revealed cytoplasmic degeneration, vacuolation and mitochondrial degeneration in both treated groups.

Conclusions: BPA showed an obvious mix of degenerative and proliferative histological changes and clear damage of the cellular organelles. This stressful condition may predispose to neoplastic changes of the vagina.

Get Citation

Keywords

bisphenol A, rat vagina, prenatal exposure, oestrogen receptors, Ki-67, ultrastructure, endocrine disrupter

About this article
Title

Effect of prenatal exposure to bisphenol a on the vagina of albino rats: immunohistochemical and ultrastructural study

Journal

Folia Morphologica

Issue

Vol 73, No 4 (2014)

Pages

399-408

Published online

2014-11-28

DOI

10.5603/FM.2014.0061

Bibliographic record

Folia Morphol 2014;73(4):399-408.

Keywords

bisphenol A
rat vagina
prenatal exposure
oestrogen receptors
Ki-67
ultrastructure
endocrine disrupter

Authors

R. A.M. Ahmed
T. A. ElGhamrawy
E.E. A. Salama

Important: This website uses cookies.tanya dokter More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl