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Minocycline but not valproic acid influence the density of NogoA-immunoreactive neurons in the hilus of the dentate gyrus of the rats subjected to intracerebral haematoma
open access
Abstract
Intracerebral haemorrhage is a devastating neurological disease with high mortality rate and poor prognosis. The most prominent manifestation of the disease arethe movement disorders, but many patients also suffer from cognitive impairment. Taking into account vulnerability of the neurons located within the hilus of the dentate gyrus (HDG) to many brain insults we decided to study the effectof experimentally induced intracerebral haematoma on density of neurons expressing NogoA protein in HDG. In addition, we studied how administration of valproic acid and minocycline, the two drugs generally believed to be neuroprotective agents, influences the density of these neurons. Our study revealed that 4 weeks after intracerebral haematoma induction, minocycline and valproic acid treatment increased the densities of NogoA-ir neurons in the hilus of contralateral dentate gyrus once the data were compared to ipsilateral hemispheres within the same group. The analysis of contralateral hemisphere data, however, revealed increased densities of NogoA-positive neurons in haematoma and valproic acidtreated animals when compared to contralateral hemispheres of control animals.The administration of minocycline was, however, able to alleviate this increase.These changes may influence the haematoma-induced reorganisation of neuronal circuitries in the dentate gyrus.
Abstract
Intracerebral haemorrhage is a devastating neurological disease with high mortality rate and poor prognosis. The most prominent manifestation of the disease arethe movement disorders, but many patients also suffer from cognitive impairment. Taking into account vulnerability of the neurons located within the hilus of the dentate gyrus (HDG) to many brain insults we decided to study the effectof experimentally induced intracerebral haematoma on density of neurons expressing NogoA protein in HDG. In addition, we studied how administration of valproic acid and minocycline, the two drugs generally believed to be neuroprotective agents, influences the density of these neurons. Our study revealed that 4 weeks after intracerebral haematoma induction, minocycline and valproic acid treatment increased the densities of NogoA-ir neurons in the hilus of contralateral dentate gyrus once the data were compared to ipsilateral hemispheres within the same group. The analysis of contralateral hemisphere data, however, revealed increased densities of NogoA-positive neurons in haematoma and valproic acidtreated animals when compared to contralateral hemispheres of control animals.The administration of minocycline was, however, able to alleviate this increase.These changes may influence the haematoma-induced reorganisation of neuronal circuitries in the dentate gyrus.
Keywords
haemorrhage, hippocampal formation, stereology


Title
Minocycline but not valproic acid influence the density of NogoA-immunoreactive neurons in the hilus of the dentate gyrus of the rats subjected to intracerebral haematoma
Journal
Issue
Pages
279-285
Published online
2014-09-05
DOI
10.5603/FM.2014.0044
Bibliographic record
Folia Morphol 2014;73(3):279-285.
Keywords
haemorrhage
hippocampal formation
stereology
Authors
K. Majak
B. Domaradzka-Pytel
P. Kowiański
J. Moryś