open access

Vol 73, No 4 (2014)
ORIGINAL ARTICLES
Published online: 2014-11-28
Submitted: 2014-02-24
Accepted: 2014-04-29
Get Citation

Three dimensional visualisation and morphometry of bone samples studied in microcomputed tomography (micro-CT)

B. Leszczyński, J. Skrzat, M. Kozerska, A. Wróbel, J. Walocha
DOI: 10.5603/FM.2014.0064
·
Folia Morphol 2014;73(4):422-428.

open access

Vol 73, No 4 (2014)
ORIGINAL ARTICLES
Published online: 2014-11-28
Submitted: 2014-02-24
Accepted: 2014-04-29

Abstract

This article highlights the utility of micro-computed tomography (micro-CT) for characterisingmicroscale bone morphology. For this purpose we tested selected samplesof the human bones (Wormian bone, rib, lumbar vertebra) to reconstruct externaland internal morphological features. Selected bony samples were investigatedusing a micro-CT scanner (Skyscan 1172, N.V., Aartselaar, Belgium). The imageresolution of scans varied from 5 to 27 μm/pixel depending on the bone sample.We used CTvox software (by Skyscan) to perform volume rendering of the samples.Further, 3-dimensional geometrical models were reconstructed using theCTvol application. Such models enabled graphical distinction between osseouscomponents of various morphology and were used to visualise the Haversian canalsystem inside the compact bone of the rib. Applying a modified transfer functionfor volume rendering we presented the overall morphology of the Wormian boneand small vascular channels penetrating its interior. As an example of quantitativeanalysis based on micro-CT scans we compared the trabecular structure of thelumbar vertebrae with CTAn software. Significant differences in percent bonevolume (BV/TV) were determined. Micro-CT was found to be a very accurate andhelpful method to study small anatomical structures of the bones in micro scale.

Abstract

This article highlights the utility of micro-computed tomography (micro-CT) for characterisingmicroscale bone morphology. For this purpose we tested selected samplesof the human bones (Wormian bone, rib, lumbar vertebra) to reconstruct externaland internal morphological features. Selected bony samples were investigatedusing a micro-CT scanner (Skyscan 1172, N.V., Aartselaar, Belgium). The imageresolution of scans varied from 5 to 27 μm/pixel depending on the bone sample.We used CTvox software (by Skyscan) to perform volume rendering of the samples.Further, 3-dimensional geometrical models were reconstructed using theCTvol application. Such models enabled graphical distinction between osseouscomponents of various morphology and were used to visualise the Haversian canalsystem inside the compact bone of the rib. Applying a modified transfer functionfor volume rendering we presented the overall morphology of the Wormian boneand small vascular channels penetrating its interior. As an example of quantitativeanalysis based on micro-CT scans we compared the trabecular structure of thelumbar vertebrae with CTAn software. Significant differences in percent bonevolume (BV/TV) were determined. Micro-CT was found to be a very accurate andhelpful method to study small anatomical structures of the bones in micro scale.

Get Citation

Keywords

bone microarchitecture, micro-computed tomography, micro-imaging

About this article
Title

Three dimensional visualisation and morphometry of bone samples studied in microcomputed tomography (micro-CT)

Journal

Folia Morphologica

Issue

Vol 73, No 4 (2014)

Pages

422-428

Published online

2014-11-28

DOI

10.5603/FM.2014.0064

Bibliographic record

Folia Morphol 2014;73(4):422-428.

Keywords

bone microarchitecture
micro-computed tomography
micro-imaging

Authors

B. Leszczyński
J. Skrzat
M. Kozerska
A. Wróbel
J. Walocha

Important: This website uses cookies. More >>

The cookies allow us to identify your computer and find out details about your last visit. They remembering whether you've visited the site before, so that you remain logged in - or to help us work out how many new website visitors we get each month. Most internet browsers accept cookies automatically, but you can change the settings of your browser to erase cookies or prevent automatic acceptance if you prefer.

By  "Via Medica sp. z o.o." sp.k., Świętokrzyska 73, 80–180 Gdańsk, Poland

tel.:+48 58 320 94 94, faks:+48 58 320 94 60, e-mail:  viamedica@viamedica.pl