Vol 76, No 4 (2017)
Original article
Published online: 2017-05-16
Immunolocalisation of nitric oxide synthase isoforms in the ductuli efferentes and epididym
Abstract
The present research used immunohistochemistry to analyse the detection and localisation of nitric oxide synthase (NOS) isoforms in the ductuli efferentes and epididymis of prepubertal and adult alpaca. In the ductuli efferentes and epididymis of prepubertal and adult animals, nNOS and eNOS were similarly expressed in epithelial lining cells, conversely differences were observed in the immunopresence of iNOS. Our data provide evidence that NOS isoforms may have roles in reproductive functions and in the developmental processes of the excurrent duct system in the alpaca.
Keywords: immunohistochemistryNOS isoformsgenital tractsalpaca
References
- Boiti C, Zerani M, Zampini D, et al. Nitric oxide synthase activity and progesterone release by isolated corpora lutea of rabbits in the early and mid-luteal phases of pseudopregnancy are modulated differently by prostaglandin E-2 and prostaglandin F-2alpha via adenylate cyclase and phospholipase C. J Endocrinol. 2000; 164(2): 179–186.
- Burnett AL, Ricker DD, Chamness SL, et al. Localization of nitric oxide synthase in the reproductive organs of the male rat. Biol Reprod. 1995; 52(1): 1–7.
- Catone G, Zerani M, Quassinti L, et al. GnRH receptor immunolocalization and in vitro GnRH effects in Leydig cells of adult alpaca (Lama pacos) testis. Reprod Dom Anim. 2010; 45(Suppl. 3): 81.
- Colitti M, Parillo F. Immunolocalization of estrogen and progesterone receptors in ewe mammary glands. Microsc Res Tech. 2013; 76(9): 955–962.
- Fowler ME. Medicine and Surgery of South American Camelids: Llama, Alpaca, Vicuňa, Guanaco. Ames I.A. ed. Iowa State University Press 1998.
- Ha Ty, Kim Hs, Shin T. Expression of constitutive endothelial, neuronal and inducible nitric oxide synthase in the testis and epididymis of horse. J Vet Med Sci. 2004; 66(4): 351–356.
- Kim HC, Byun JS, Lee TK, et al. Expression of nitric oxide synthase isoforms in the testes of pigs. Anat Histol Embryol. 2007; 36(2): 135–138.
- McCann SM, Mastronardi C, de Laurentiis A, et al. The nitric oxide theory of aging revisited. Ann N Y Acad Sci. 2005; 1057: 64–84.
- Meiser H, Schulz R. Detection and localization of two constitutive NOS isoforms in bull spermatozoa. Anat Histol Embryol. 2003; 32(6): 321–325.
- Parillo F, Verini Supplizi A, Macrì D, et al. The ductus epididymis of the alpaca: immunohistochemical and lectin histochemical study. Reprod Domest Anim. 2009a; 44(2): 284–294.
- Parillo F, Magi GE, Diverio S, et al. Immunohistochemical and lectin histochemical analysis of the alpaca efferent ducts. Histol Histopathol. 2009; 24(1): 1–12.
- Parillo F, Verini Su, Scrollavezza P, et al. Immunolocalization of ciclooxygenases in the ductuli efferentes and epididymis of pre-pubertal and adult alpaca (Lama pacos). Reprod Dom Anim. 2009c; 44(Suppl. 3): 119.
- Parillo F, Catone G, Boiti C, et al. Immunopresence and enzymatic activity of nitric oxide synthases, cyclooxygenases and PGE2-9-ketoreductase and in vitro production of PGF2α, PGE2 and testosterone in the testis of adult and prepubertal alpaca (Lama pacos). Gen Comp Endocrinol. 2011; 171(3): 381–388.
- Parillo F, Verini Supplizi A, Mancuso R, et al. Glycomolecule modifications in the seminiferous epithelial cells and in the acrosome of post-testicular spermatozoa in the alpaca. Reprod Domest Anim. 2012; 47(4): 675–686.
- Parillo F, Catone G, Maranesi M, et al. Immunolocalization, gene expression, and enzymatic activity of cyclooxygenases, prostaglandin e2-9-ketoreductase, and nitric oxide synthases in Mediterranean buffalo (Bubalus bubalis) corpora lutea during diestrus. Microsc Res Tech. 2012; 75(12): 1682–1690.
- Parillo F, Catone G, Gobbetti A, et al. Cell localization of ACTH, dopamine, and GnRH receptors and PPARγ in bovine corpora lutea during diestrus. Acta Sci Vet. 2013; 41: e1129.
- Parillo F, Dall'Aglio C, Brecchia G, et al. Aglepristone (RU534) effects on luteal function of pseudopregnant rabbits: steroid receptors, enzymatic activities, and hormone productions in corpus luteum and uterus. Anim Reprod Sci. 2013; 138(1-2): 118–132.
- Parillo F, Maranesi M, Brecchia G, et al. Immunohistochemial study on effectors of ovary medulla blood vessel in pseudopregnant rabbit. Acta Sci Vet. 2013; (in press).
- Parillo F, Catone G, Capezzone C, et al. Presence of immunoreactive cyclooxygenases in the ductuli efferentes and epididymis of prepubertal and adult alpaca (Lama pacos). Vet Arhiv. 2013; (in press).
- Parillo F, Zerani M, Maranesi M, et al. Ovarian hormones and fasting differentially regulate pituitary receptors for estrogen and gonadotropin-releasing hormone in rabbit female. Microsc Res Tech. 2014; 77(3): 201–210.
- Tibary A, Vaughan J. Reproductive physiology and infertility in male South American camelids: A review and clinical observations. Small Ruminant Research. 2006; 61(2-3): 283–298.
- Yang JZ, Ajonuma LC, Rowlands DK, et al. The role of inducible nitric oxide synthase in gamete interaction and fertilization: a comparative study on knockout mice of three NOS isoforms. Cell Biol Int. 2005; 29(9): 785–791.
- Zini A, O'Bryan MK, Magid MS, et al. Immunohistochemical localization of endothelial nitric oxide synthase in human testis, epididymis, and vas deferens suggests a possible role for nitric oxide in spermatogenesis, sperm maturation, and programmed cell death. Biol Reprod. 1996; 55(5): 935–941.
- Zerani M, Catone G, Quassinti L, et al. In vitro effects of gonadotropin-releasing hormone (GnRH) on Leydig cells of adult alpaca (Lama pacos) testis: GnRH receptor immunolocalization, testosterone and prostaglandin synthesis, and cyclooxygenase activities. Domest Anim Endocrinol. 2011; 40(1): 51–59.
- Zerani M, Catone G, Maranesi M, et al. Gonadotropin-releasing hormone 1 directly affects corpora lutea lifespan in Mediterranean buffalo (Bubalus bubalis) during diestrus: presence and in vitro effects on enzymatic and hormonal activities. Biol Reprod. 2012; 87(2): 45.
- Zerani M, Catone G, Betti G, et al. Immunopresence and functional activity of prostaglandin-endoperoxide synthases and nitric oxide synthases in bovine corpora lutea during diestrus. Folia Morphol. 2013; 72(1): 36–40.
- Zerani M, Maranesi M, Brecchia G, et al. Evidence for a luteotropic role of peroxisome proliferator-activated receptor gamma: expression and in vitro effects on enzymatic and hormonal activities in corpora lutea of pseudopregnant rabbits. Biol Reprod. 2013; 88(3): 62.
