open access

Vol 72, No 4 (2013)
ORIGINAL ARTICLES
Published online: 2013-12-09
Submitted: 2013-03-20
Accepted: 2013-04-14
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Cholinergic innervation of human mesenteric lymphatic vessels

V. D’Andrea, E. Bianchi, S. Taurone, F. Mignini, C. Cavallotti, M. Artico
DOI: 10.5603/FM.2013.0054
·
Folia Morphol 2013;72(4):322-327.

open access

Vol 72, No 4 (2013)
ORIGINAL ARTICLES
Published online: 2013-12-09
Submitted: 2013-03-20
Accepted: 2013-04-14

Abstract

Background: The cholinergic neurotransmission within the human mesenteric lymphatic vessels has been poorly studied. Therefore, our aim is to analyse the cholinergic nerve fibres of lymphatic vessels using the traditional enzymatic techniques of staining, plus the biochemical modifications of acetylcholinesterase (AChE) activity.

Materials and methods: Specimens obtained from human mesenteric lymphatic vessels were subjected to the following experimental procedures: 1) drawing, cutting and staining of tissues; 2) staining of total nerve fibres; 3) enzymatic staining of cholinergic nerve fibres; 4) homogenisation of tissues; 5) biochemical amount of proteins; 6) biochemical amount of AChE activity; 6) quantitative analysis of images; 7) statistical analysis of data.

Results: The mesenteric lymphatic vessels show many AChE positive nerve fibres around their wall with an almost plexiform distribution. The incubation time was performed at 1 h (partial activity) and 6 h (total activity). Moreover, biochemical dosage of the same enzymatic activity confirms the results obtained with morphological methods.

Conclusions: The homogenates of the studied tissues contain strong AChE activity. In our study, the lymphatic vessels appeared to contain few cholinergic nerve fibres. Therefore, it is expected that perivascular nerve stimulation stimulates cholinergic nerves innervating the mesenteric arteries to release the neurotransmitter AChE, which activates muscarinic or nicotinic receptors to modulate adrenergic neurotransmission. These results strongly suggest, that perivascular cholinergic nerves have little or no effect on the adrenergic nerve function in mesenteric arteries. The cholinergic nerves innervating mesenteric arteries do not mediate direct vascular responses.

Abstract

Background: The cholinergic neurotransmission within the human mesenteric lymphatic vessels has been poorly studied. Therefore, our aim is to analyse the cholinergic nerve fibres of lymphatic vessels using the traditional enzymatic techniques of staining, plus the biochemical modifications of acetylcholinesterase (AChE) activity.

Materials and methods: Specimens obtained from human mesenteric lymphatic vessels were subjected to the following experimental procedures: 1) drawing, cutting and staining of tissues; 2) staining of total nerve fibres; 3) enzymatic staining of cholinergic nerve fibres; 4) homogenisation of tissues; 5) biochemical amount of proteins; 6) biochemical amount of AChE activity; 6) quantitative analysis of images; 7) statistical analysis of data.

Results: The mesenteric lymphatic vessels show many AChE positive nerve fibres around their wall with an almost plexiform distribution. The incubation time was performed at 1 h (partial activity) and 6 h (total activity). Moreover, biochemical dosage of the same enzymatic activity confirms the results obtained with morphological methods.

Conclusions: The homogenates of the studied tissues contain strong AChE activity. In our study, the lymphatic vessels appeared to contain few cholinergic nerve fibres. Therefore, it is expected that perivascular nerve stimulation stimulates cholinergic nerves innervating the mesenteric arteries to release the neurotransmitter AChE, which activates muscarinic or nicotinic receptors to modulate adrenergic neurotransmission. These results strongly suggest, that perivascular cholinergic nerves have little or no effect on the adrenergic nerve function in mesenteric arteries. The cholinergic nerves innervating mesenteric arteries do not mediate direct vascular responses.

Get Citation

Keywords

cholinergic nerve fibres, neurotransmitter, acetylcholinesterase, mesenteric vessels

About this article
Title

Cholinergic innervation of human mesenteric lymphatic vessels

Journal

Folia Morphologica

Issue

Vol 72, No 4 (2013)

Pages

322-327

Published online

2013-12-09

DOI

10.5603/FM.2013.0054

Bibliographic record

Folia Morphol 2013;72(4):322-327.

Keywords

cholinergic nerve fibres
neurotransmitter
acetylcholinesterase
mesenteric vessels

Authors

V. D’Andrea
E. Bianchi
S. Taurone
F. Mignini
C. Cavallotti
M. Artico

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