open access

Vol 72, No 1 (2013)
ORIGINAL ARTICLES
Published online: 2013-03-05
Submitted: 2012-11-20
Accepted: 2012-12-17
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Immunopresence and functional activity of prostaglandin-endoperoxide synthases and nitric oxide synthases in bovine corpora lutea during diestrus

M. Zerani, G. Catone, G. Betti, F. Parillo
DOI: 10.5603/FM.2013.0006
·
Folia Morphol 2013;72(1):36-40.

open access

Vol 72, No 1 (2013)
ORIGINAL ARTICLES
Published online: 2013-03-05
Submitted: 2012-11-20
Accepted: 2012-12-17

Abstract

The aim of this study was to evaluate the occurrence and the activity of prostaglandin-endoperoxide synthase 1 (PTGS1), PTGS2, and endothelial, neuronal, and inducible nitric oxide synthase (e-, n-, and iNOS) in early, mid, late, and regressive corpora lutea (CL) of bovines during diestrus. PTGS1 immunoreactivity
was localised mainly in the cytoplasm of small luteal cells, whereas PTGS2 was detected in the cytoplasm of large luteal cells during early, mid, and late stages. The immunoexpression of all NOS isoforms was observed in the nuclei of luteal cells in the CL stages examined. PTGS1 enzyme activity was higher in late CL and lower in regressive ones; PTGS2 increased from early to late CL and
lowered in regressive ones. Constitutive NOS enzymatic activity (eNOS plus nNOS) was higher in late CL and lower in regressive ones; iNOS was lower in regressive CL. These results support the idea that PTGSs and NOSs regulate the bovine CL life span mainly during the transition from the luteotrophic to the luteolytic phase.

Abstract

The aim of this study was to evaluate the occurrence and the activity of prostaglandin-endoperoxide synthase 1 (PTGS1), PTGS2, and endothelial, neuronal, and inducible nitric oxide synthase (e-, n-, and iNOS) in early, mid, late, and regressive corpora lutea (CL) of bovines during diestrus. PTGS1 immunoreactivity
was localised mainly in the cytoplasm of small luteal cells, whereas PTGS2 was detected in the cytoplasm of large luteal cells during early, mid, and late stages. The immunoexpression of all NOS isoforms was observed in the nuclei of luteal cells in the CL stages examined. PTGS1 enzyme activity was higher in late CL and lower in regressive ones; PTGS2 increased from early to late CL and
lowered in regressive ones. Constitutive NOS enzymatic activity (eNOS plus nNOS) was higher in late CL and lower in regressive ones; iNOS was lower in regressive CL. These results support the idea that PTGSs and NOSs regulate the bovine CL life span mainly during the transition from the luteotrophic to the luteolytic phase.
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Keywords

cattle; estrous cycle; ovary; reproduction

About this article
Title

Immunopresence and functional activity of prostaglandin-endoperoxide synthases and nitric oxide synthases in bovine corpora lutea during diestrus

Journal

Folia Morphologica

Issue

Vol 72, No 1 (2013)

Pages

36-40

Published online

2013-03-05

DOI

10.5603/FM.2013.0006

Bibliographic record

Folia Morphol 2013;72(1):36-40.

Keywords

cattle
estrous cycle
ovary
reproduction

Authors

M. Zerani
G. Catone
G. Betti
F. Parillo

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