open access

Vol 70, No 3 (2011)
ORIGINAL ARTICLES
Published online: 2011-08-24
Submitted: 2012-06-27
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Biocompatibility of bone graft substitutes: effects on survival and proliferation of porcine multilineage stem cells < i > in vitro

C.E. Zimmermann, M. Gierloff, J. Hedderich, Y. Açil, J. Wiltfang, H. Terheyden
Folia Morphol 2011;70(3):154-160.

open access

Vol 70, No 3 (2011)
ORIGINAL ARTICLES
Published online: 2011-08-24
Submitted: 2012-06-27

Abstract

Bone graft substitutes (BGS) are widely used in clinical practice. For stem cellbased approaches to bone tissue engineering BGS need to show sufficient biocompatibility in the in vitro setting. This study was designed to demonstrate the influence of six different BGS on the proliferation and metabolic activity of porcine mesenchymal multilineage stem cells (pMSC) in vitro.
Bone-marrow derived pMSC were cultivated for 24 hours with the eluates of six different BGS. The eluates were generated by incubating the BGS three times in succession for 24 hours with a culture medium and collecting the supernatants. pMSC vitality and proliferation in the presence of eluates from the first, second, and third incubation were assessed by WST-test quantification of metabolically active cells.
Culture of pMSC with eluates in all cases resulted in decreased cell numbers in an eluate concentration-dependent manner. At least a 65% loss of cells compared to controls (culture medium without eluates) could be observed in the presence of undiluted eluates. The negative influence of eluates varied significantly among BGS. In all cases, second and third eluates were less potent in their negative effects on cellular vitality/proliferation.
In conclusion, the BGS examined here should be submitted to thorough preincubation before in vitro use for cell-based constructs to maximize cell viability for the tissue engineering of bone. (Folia Morphol 2011; 70, 3: 154–160)

Abstract

Bone graft substitutes (BGS) are widely used in clinical practice. For stem cellbased approaches to bone tissue engineering BGS need to show sufficient biocompatibility in the in vitro setting. This study was designed to demonstrate the influence of six different BGS on the proliferation and metabolic activity of porcine mesenchymal multilineage stem cells (pMSC) in vitro.
Bone-marrow derived pMSC were cultivated for 24 hours with the eluates of six different BGS. The eluates were generated by incubating the BGS three times in succession for 24 hours with a culture medium and collecting the supernatants. pMSC vitality and proliferation in the presence of eluates from the first, second, and third incubation were assessed by WST-test quantification of metabolically active cells.
Culture of pMSC with eluates in all cases resulted in decreased cell numbers in an eluate concentration-dependent manner. At least a 65% loss of cells compared to controls (culture medium without eluates) could be observed in the presence of undiluted eluates. The negative influence of eluates varied significantly among BGS. In all cases, second and third eluates were less potent in their negative effects on cellular vitality/proliferation.
In conclusion, the BGS examined here should be submitted to thorough preincubation before in vitro use for cell-based constructs to maximize cell viability for the tissue engineering of bone. (Folia Morphol 2011; 70, 3: 154–160)
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Keywords

bone tissue engineering; cell viability; cytotoxicity; mesenchymal stem cells

About this article
Title

Biocompatibility of bone graft substitutes: effects on survival and proliferation of porcine multilineage stem cells in vitro

Journal

Folia Morphologica

Issue

Vol 70, No 3 (2011)

Pages

154-160

Published online

2011-08-24

Bibliographic record

Folia Morphol 2011;70(3):154-160.

Keywords

bone tissue engineering
cell viability
cytotoxicity
mesenchymal stem cells

Authors

C.E. Zimmermann
M. Gierloff
J. Hedderich
Y. Açil
J. Wiltfang
H. Terheyden

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