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Biocompatibility of bone graft substitutes: effects on survival and proliferation of porcine multilineage stem cells < i > in vitro
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Abstract
Bone-marrow derived pMSC were cultivated for 24 hours with the eluates of six different BGS. The eluates were generated by incubating the BGS three times in succession for 24 hours with a culture medium and collecting the supernatants. pMSC vitality and proliferation in the presence of eluates from the first, second, and third incubation were assessed by WST-test quantification of metabolically active cells.
Culture of pMSC with eluates in all cases resulted in decreased cell numbers in an eluate concentration-dependent manner. At least a 65% loss of cells compared to controls (culture medium without eluates) could be observed in the presence of undiluted eluates. The negative influence of eluates varied significantly among BGS. In all cases, second and third eluates were less potent in their negative effects on cellular vitality/proliferation.
In conclusion, the BGS examined here should be submitted to thorough preincubation before in vitro use for cell-based constructs to maximize cell viability for the tissue engineering of bone. (Folia Morphol 2011; 70, 3: 154–160)
Abstract
Bone-marrow derived pMSC were cultivated for 24 hours with the eluates of six different BGS. The eluates were generated by incubating the BGS three times in succession for 24 hours with a culture medium and collecting the supernatants. pMSC vitality and proliferation in the presence of eluates from the first, second, and third incubation were assessed by WST-test quantification of metabolically active cells.
Culture of pMSC with eluates in all cases resulted in decreased cell numbers in an eluate concentration-dependent manner. At least a 65% loss of cells compared to controls (culture medium without eluates) could be observed in the presence of undiluted eluates. The negative influence of eluates varied significantly among BGS. In all cases, second and third eluates were less potent in their negative effects on cellular vitality/proliferation.
In conclusion, the BGS examined here should be submitted to thorough preincubation before in vitro use for cell-based constructs to maximize cell viability for the tissue engineering of bone. (Folia Morphol 2011; 70, 3: 154–160)
Keywords
bone tissue engineering; cell viability; cytotoxicity; mesenchymal stem cells
Title
Biocompatibility of bone graft substitutes: effects on survival and proliferation of porcine multilineage stem cells in vitro
Journal
Issue
Article type
Original article
Pages
154-160
Published online
2011-08-24
Page views
571
Article views/downloads
1854
Bibliographic record
Folia Morphol 2011;70(3):154-160.
Keywords
bone tissue engineering
cell viability
cytotoxicity
mesenchymal stem cells
Authors
C.E. Zimmermann
M. Gierloff
J. Hedderich
Y. Açil
J. Wiltfang
H. Terheyden