open access

Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Published online: 2003-09-05
Submitted: 2012-02-06
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A quantitative analysis of the expression of alpha-smooth muscle actin in mesangioproliferative (GnMes) glomerulonephritis

Edyta Nieruchalska, Rafał Strzelczyk, Aldona Woźniak, Jakub Żurawski, Elżbieta Kaczmarek, Wiesława Salwa-Żurawska
Folia Morphol 2003;62(4):451-453.

open access

Vol 62, No 4 (2003)
SHORT COMMUNICATIONS
Published online: 2003-09-05
Submitted: 2012-02-06

Abstract

The aim of the study was to quantitatively assess the expression of alpha-SMA in the renal interstitium in GnMes cases. Two image analysis approaches (in grey scale and in spatial colour images) were used to determine correctly the presence of the reaction. In spatial evaluation, the mean area fraction of a-SMA in the renal interstitium was 3.63% ± 1.29%, while the results obtained from traditional quantitative method based on grey scale images were biased and too low.

Abstract

The aim of the study was to quantitatively assess the expression of alpha-SMA in the renal interstitium in GnMes cases. Two image analysis approaches (in grey scale and in spatial colour images) were used to determine correctly the presence of the reaction. In spatial evaluation, the mean area fraction of a-SMA in the renal interstitium was 3.63% ± 1.29%, while the results obtained from traditional quantitative method based on grey scale images were biased and too low.
Get Citation

Keywords

alpha-SMA; mesangioproliferative glomerulonephritis; immunohistochemistry; interstitial fibrosis

About this article
Title

A quantitative analysis of the expression of alpha-smooth muscle actin in mesangioproliferative (GnMes) glomerulonephritis

Journal

Folia Morphologica

Issue

Vol 62, No 4 (2003)

Pages

451-453

Published online

2003-09-05

Bibliographic record

Folia Morphol 2003;62(4):451-453.

Keywords

alpha-SMA
mesangioproliferative glomerulonephritis
immunohistochemistry
interstitial fibrosis

Authors

Edyta Nieruchalska
Rafał Strzelczyk
Aldona Woźniak
Jakub Żurawski
Elżbieta Kaczmarek
Wiesława Salwa-Żurawska

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