open access

Vol 63, No 1 (2004)
SHORT COMMUNICATIONS
Published online: 2003-12-03
Submitted: 2012-02-06
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AgNOR, Ki-67 and PCNA expression in fibroepithelial tumours of the breast in correlation with morphological features

Małgorzata Barwijuk-Machała, Bogusław Musiatowicz, Jacek Cylwik, Joanna Reszeć, Albert Augustynowicz
Folia Morphol 2004;63(1):133-135.

open access

Vol 63, No 1 (2004)
SHORT COMMUNICATIONS
Published online: 2003-12-03
Submitted: 2012-02-06

Abstract

The authors retrospectively reviewed the cytological slides of 44 histopathologically confirmed fibroepithelial lesions of the breast, of which 11 were fibroadenoma (FA), 19 benign phyllodes tumours (PTLGM), 8 borderline (PTBM) and 6 malignant (PTHGM). The 2 FA misdiagnosed as PTLGM in cytological smears were both of cellular type. NORS were quantified in a series of the above cases using the silver-colloid method. Expression of Ki-67 and PCNA were evaluated by immunohistochemistry on sections from the corresponding paraffin blocks. The results were compared with morphological parameters.
In phyllodes tumours (PT), the AgNOR scores showed a tendency to increase with degrees of malignancy. There was significant correlation between Ag- NOR counts and proliferation rates as determined by Ki-67 and PCNA immunostaining. Ki-67 and PCNA expression correlated with mitotic count, stromal overgrowth, cellularity and atypia in PT. Determination of the AgNOR number per cell revealed an overlap between FA and PTLGM. The proliferating activity determined by immunohistochemistry with Ki-67 and PCNA antibodies did not reveal any significant difference between FA and PTLGM.
In summary, Ki-67 and PCNA expression is suggested as a marker of stromal element proliferation. The results obtained confirm the diagnostic difficulties in distinguishing PTLGM from FA of the cellular type using fine needle aspiration cytology.

Abstract

The authors retrospectively reviewed the cytological slides of 44 histopathologically confirmed fibroepithelial lesions of the breast, of which 11 were fibroadenoma (FA), 19 benign phyllodes tumours (PTLGM), 8 borderline (PTBM) and 6 malignant (PTHGM). The 2 FA misdiagnosed as PTLGM in cytological smears were both of cellular type. NORS were quantified in a series of the above cases using the silver-colloid method. Expression of Ki-67 and PCNA were evaluated by immunohistochemistry on sections from the corresponding paraffin blocks. The results were compared with morphological parameters.
In phyllodes tumours (PT), the AgNOR scores showed a tendency to increase with degrees of malignancy. There was significant correlation between Ag- NOR counts and proliferation rates as determined by Ki-67 and PCNA immunostaining. Ki-67 and PCNA expression correlated with mitotic count, stromal overgrowth, cellularity and atypia in PT. Determination of the AgNOR number per cell revealed an overlap between FA and PTLGM. The proliferating activity determined by immunohistochemistry with Ki-67 and PCNA antibodies did not reveal any significant difference between FA and PTLGM.
In summary, Ki-67 and PCNA expression is suggested as a marker of stromal element proliferation. The results obtained confirm the diagnostic difficulties in distinguishing PTLGM from FA of the cellular type using fine needle aspiration cytology.
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Keywords

AgNORs ; Ki-67; PCNA; fibroepithelial tumours of the breast

About this article
Title

AgNOR, Ki-67 and PCNA expression in fibroepithelial tumours of the breast in correlation with morphological features

Journal

Folia Morphologica

Issue

Vol 63, No 1 (2004)

Pages

133-135

Published online

2003-12-03

Bibliographic record

Folia Morphol 2004;63(1):133-135.

Keywords

AgNORs
Ki-67
PCNA
fibroepithelial tumours of the breast

Authors

Małgorzata Barwijuk-Machała
Bogusław Musiatowicz
Jacek Cylwik
Joanna Reszeć
Albert Augustynowicz

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