open access

Vol 65, No 3 (2006)
ORIGINAL ARTICLES
Published online: 2006-06-08
Submitted: 2012-02-06
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Vanilloid receptor type 1-immunoreactive nerves in the rat urinary bladder and primary afferent neurones: the effects of age

H.A.M. Saleh
Folia Morphol 2006;65(3):213-220.

open access

Vol 65, No 3 (2006)
ORIGINAL ARTICLES
Published online: 2006-06-08
Submitted: 2012-02-06

Abstract

The vanilloid receptor (VR1) is a molecular integrator of various painful stimuli, including capsaicin, acid and high temperature. VR1 protein functions both as a receptor for capsaicin and a transducer of noxious thermal stimuli. In addition, VR1 is well characterised at the terminals of sensory nerves involved in the pain pathway. VR1 is also expressed in a capsaicin-sensitive and peptide-containing sub-population of primary sensory nerves.
Indirect immunohistochemistry was used to examine the distribution of nerves immunoreactive (ir) for VR1 in the base of the urinary bladder and in the neurones of the lumbosacral dorsal root ganglia (L1-L2 and L6-S1) of young adult (3 months) and aged (24 months) male rats. Semi-quantitative estimations of nerve densities were assessed and quantitative studies were also used to examine the effects of age on the percentage of VR1-ir dorsal root ganglion neurones. The bladder base in young adults showed dense VR1-ir fibres within the urothelium and in the subepithelium and fibres ranging from sparse to moderate in number in the muscle coat. In comparison to the young animals, the aged rats showed sparse to moderate densities of VR1-ir nerves in the subepithelium and sparse fibres in the muscle layers. In the lumbosacral dorsal root ganglia the percentage of VR1-ir neuronal profiles showed a significant reduction from (mean ± SEM) 17.8 ± 2% in the young adult to 12 ± 1.6 in the aged rats. The present findings suggest that the effects of VR1 on bladder function (nociception and reflex micturition) are influenced by age and the reduction with age of VR1-ir neurones in the dorsal root ganglia could also have important implications for the micturition reflex.

Abstract

The vanilloid receptor (VR1) is a molecular integrator of various painful stimuli, including capsaicin, acid and high temperature. VR1 protein functions both as a receptor for capsaicin and a transducer of noxious thermal stimuli. In addition, VR1 is well characterised at the terminals of sensory nerves involved in the pain pathway. VR1 is also expressed in a capsaicin-sensitive and peptide-containing sub-population of primary sensory nerves.
Indirect immunohistochemistry was used to examine the distribution of nerves immunoreactive (ir) for VR1 in the base of the urinary bladder and in the neurones of the lumbosacral dorsal root ganglia (L1-L2 and L6-S1) of young adult (3 months) and aged (24 months) male rats. Semi-quantitative estimations of nerve densities were assessed and quantitative studies were also used to examine the effects of age on the percentage of VR1-ir dorsal root ganglion neurones. The bladder base in young adults showed dense VR1-ir fibres within the urothelium and in the subepithelium and fibres ranging from sparse to moderate in number in the muscle coat. In comparison to the young animals, the aged rats showed sparse to moderate densities of VR1-ir nerves in the subepithelium and sparse fibres in the muscle layers. In the lumbosacral dorsal root ganglia the percentage of VR1-ir neuronal profiles showed a significant reduction from (mean ± SEM) 17.8 ± 2% in the young adult to 12 ± 1.6 in the aged rats. The present findings suggest that the effects of VR1 on bladder function (nociception and reflex micturition) are influenced by age and the reduction with age of VR1-ir neurones in the dorsal root ganglia could also have important implications for the micturition reflex.
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Keywords

VR1; immunohistochemistry; sensory neurones; dorsal root ganglia

About this article
Title

Vanilloid receptor type 1-immunoreactive nerves in the rat urinary bladder and primary afferent neurones: the effects of age

Journal

Folia Morphologica

Issue

Vol 65, No 3 (2006)

Pages

213-220

Published online

2006-06-08

Bibliographic record

Folia Morphol 2006;65(3):213-220.

Keywords

VR1
immunohistochemistry
sensory neurones
dorsal root ganglia

Authors

H.A.M. Saleh

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