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Morphometric alterations of the rat spleen following formaldehyde exposure
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Abstract
A total of 28 albino Wistar rats aged 6-7 postnatal weeks were divided into the following three case groups according to their exposure to formaldehyde: E1 (2 h/day, 2 days/week), E2 (2 h/day, 4 days/week), E3 (4 h/day, 4 days/week) and one control group. When the exposure period had expired the animals were anaesthetised with chloroform. After cervical dislocation, the abdomen was dissected and spleen specimens were taken. These were sectioned and stained with the haematoxylin and eosin technique for morphometric study. Data was obtained from an Olympus light microscope and then analysed with SPSS (version 11.5) and one-way ANOVA test.
The white pulp area and diameter and the marginal zone diameter were greater in group E3 than those in the other groups. The germinal centre area and diameter and the diameter of the periarterial lymphoid sheaths (PALS) were greater in group E2 than in other groups, although there was no significant difference between groups in the area of white pulp and the PALS diameter (p < 0.05).
This study showed that formaldehyde vapour can cause morphometric changes in the white pulp of the spleen in rats
(Folia Morphol 2008; 67: 19-23).
Abstract
A total of 28 albino Wistar rats aged 6-7 postnatal weeks were divided into the following three case groups according to their exposure to formaldehyde: E1 (2 h/day, 2 days/week), E2 (2 h/day, 4 days/week), E3 (4 h/day, 4 days/week) and one control group. When the exposure period had expired the animals were anaesthetised with chloroform. After cervical dislocation, the abdomen was dissected and spleen specimens were taken. These were sectioned and stained with the haematoxylin and eosin technique for morphometric study. Data was obtained from an Olympus light microscope and then analysed with SPSS (version 11.5) and one-way ANOVA test.
The white pulp area and diameter and the marginal zone diameter were greater in group E3 than those in the other groups. The germinal centre area and diameter and the diameter of the periarterial lymphoid sheaths (PALS) were greater in group E2 than in other groups, although there was no significant difference between groups in the area of white pulp and the PALS diameter (p < 0.05).
This study showed that formaldehyde vapour can cause morphometric changes in the white pulp of the spleen in rats
(Folia Morphol 2008; 67: 19-23).
Keywords
white pulp; periarterial lymphoid sheaths; follicle; germinal centre area; marginal zone diameter; mantle zone
About this articleTitle
Morphometric alterations of the rat spleen following formaldehyde exposure
Journal
Issue
Article type
Original article
Pages
19-23
Published online
2007-12-03
Page views
559
Article views/downloads
1204
Bibliographic record
Folia Morphol 2008;67(1):19-23.
Keywords
white pulp
periarterial lymphoid sheaths
follicle
germinal centre area
marginal zone diameter
mantle zone
Authors
M.J. Golalipour
H. Kord
S. Ghafari
A.M. Gharravi
A. Davarian
S.A. Fazeli
R. Azarhoush
