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Vol 58, No 3 (2020)
Original paper
Submitted: 2020-03-19
Accepted: 2020-09-13
Published online: 2020-09-22
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Wilms’ tumor 1 antigen immunoreactivity in epithelial ovarian cancer — diagnostic and prognostic value

Elzbieta Zarychta1, Katarzyna Lepinay1, Sebastian Szubert1, Jakub Jozwicki2, Jan Misiak3, Anna A. Brozyna4, Katarzyna Kosinska-Kaczynska1, Agnieszka Lewandowska1, Ewa Malicka1, Adrianna Makarewicz5, Piotr Rhone6, Wojciech Jozwicki3
DOI: 10.5603/FHC.a2020.0022
·
Pubmed: 32960974
·
Folia Histochem Cytobiol 2020;58(3):198-207.
Affiliations
  1. Second Department of Obstetrics and Gynecology, Center of Postgraduate Medical Education, Warsaw, Poland
  2. Department of Clinical Pathomorphology, Faculty of Medicine, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland
  3. Department of Tumor Pathology and Pathomorphology, Department of Oncology, Faculty of Health Sciences, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Torun, Poland, Torun, Poland
  4. Department of Human Biology, Institute of Biology, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University, Torun, Poland
  5. Department of Oncology, The Franciszek Lukaszczyk Oncological Center, Bydgoszcz, Poland
  6. Department of Clinical Breast Cancer and Reconstructive Surgery, The Franciszek Lukaszczyk Oncological Center,, Bydgoszcz, Poland

open access

Vol 58, No 3 (2020)
ORIGINAL PAPERS
Submitted: 2020-03-19
Accepted: 2020-09-13
Published online: 2020-09-22

Abstract

Objectives. Ovarian cancer is a heterogeneous disease, with a number of different histological subtypes with various responses to treatment. Wilms’ tumor 1 (WT1) immunoreactivity is used to distinguish between OC’s various subtypes. However, little is known about the protein’s role as a prognostic factor. Thus, the main aim of our study was to evaluate the relationship between WT1 expression and patient overall survival (OS) and lymph node metastases. Materials and methods. Study group consisted of 164 women aged 22–84, diagnosed with epithelial ovarian cancer (EOC). WT1 expression in histological slides was assessed by immunohistochemistry. Results. Serous tumors were the most common subtype among EOC (n = 126; 76.8%), followed by endometrioid (n = 20; 12.2%), clear-cell (n = 14; 8.5%) and mucinous cancer (n = 4; 2.4%). Of all serous EOC, WT1-positive tumors accounted for 75.6% of cases and this number was significantly higher than in other histological subtypes (p < 0.0001). Patients with lymph node metastases were more likely to have WT1-positive than WT1-negative tumors (p = 0.006). There was no significant correlation between WT1 immunoreactivity and OS across the whole study group of EOC patients (p = 0.6); however, in the group of non-serous (mucinous, endometrioid and clear-cell) EOC subjects, WT1 immunoreactivity was associated with shorter OS (p = 0.046). Conclusions. WT1 immunoreactivity may be helpful in differentiating primary epithelial serous carcinomas from non-serous ovarian cancers; however, its prognostic role in EOC is rather uncertain.

Abstract

Objectives. Ovarian cancer is a heterogeneous disease, with a number of different histological subtypes with various responses to treatment. Wilms’ tumor 1 (WT1) immunoreactivity is used to distinguish between OC’s various subtypes. However, little is known about the protein’s role as a prognostic factor. Thus, the main aim of our study was to evaluate the relationship between WT1 expression and patient overall survival (OS) and lymph node metastases. Materials and methods. Study group consisted of 164 women aged 22–84, diagnosed with epithelial ovarian cancer (EOC). WT1 expression in histological slides was assessed by immunohistochemistry. Results. Serous tumors were the most common subtype among EOC (n = 126; 76.8%), followed by endometrioid (n = 20; 12.2%), clear-cell (n = 14; 8.5%) and mucinous cancer (n = 4; 2.4%). Of all serous EOC, WT1-positive tumors accounted for 75.6% of cases and this number was significantly higher than in other histological subtypes (p < 0.0001). Patients with lymph node metastases were more likely to have WT1-positive than WT1-negative tumors (p = 0.006). There was no significant correlation between WT1 immunoreactivity and OS across the whole study group of EOC patients (p = 0.6); however, in the group of non-serous (mucinous, endometrioid and clear-cell) EOC subjects, WT1 immunoreactivity was associated with shorter OS (p = 0.046). Conclusions. WT1 immunoreactivity may be helpful in differentiating primary epithelial serous carcinomas from non-serous ovarian cancers; however, its prognostic role in EOC is rather uncertain.

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Keywords

epithelial ovarian cancer; WT1; Wilms’ tumor antigen 1; lymph node metastases

About this article
Title

Wilms’ tumor 1 antigen immunoreactivity in epithelial ovarian cancer — diagnostic and prognostic value

Journal

Folia Histochemica et Cytobiologica

Issue

Vol 58, No 3 (2020)

Article type

Original paper

Pages

198-207

Published online

2020-09-22

Page views

1113

Article views/downloads

881

DOI

10.5603/FHC.a2020.0022

Pubmed

32960974

Bibliographic record

Folia Histochem Cytobiol 2020;58(3):198-207.

Keywords

epithelial ovarian cancer
WT1
Wilms’ tumor antigen 1
lymph node metastases

Authors

Elzbieta Zarychta
Katarzyna Lepinay
Sebastian Szubert
Jakub Jozwicki
Jan Misiak
Anna A. Brozyna
Katarzyna Kosinska-Kaczynska
Agnieszka Lewandowska
Ewa Malicka
Adrianna Makarewicz
Piotr Rhone
Wojciech Jozwicki

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